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AB216136

Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120]

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(2 Publications)

Mouse Monoclonal EPCAM antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPCAM.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

4 Images
Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)

Overlay histogram showing MCF-7 (Human breast adenocarcinoma) cells stained with ab216136 (blue) or isotype control (red). The cells were fixed with paraformaldehyde and incubated with ab216136 at 1 ug/million cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)

Immunohistochemical analysis of paraffin embedded, formalin fixed human breast carcinoma tissue labeling EpCAM with ab216136 at 1 μg/mL.

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)
  • WB

Lab

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)

Lanes 1 - 4 : Merged signal (red and green). Green - ab216136 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

ab216136 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EPCAM knockout sample. Wild-type and EPCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab216136 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136) at 0.5 µg/mL

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

EPCAM knockout A431 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 35 kDa

Observed band size: 40 kDa

false

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)
  • WB

Supplier Data

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136)

All lanes:

Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

All lanes:

Human lung tissue lysate

Predicted band size: 35 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Cocktail EGP40/826/837/1110/1120

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EPCAM.

P16422

Specificity

This antibody recognizes extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (Ep-CAM).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
See full target information EPCAM
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed41009720

2025

p.N370S Mutation Influences the Morphology and Lipid Composition of Extracellular Vesicles in Blood Plasma from Patients with Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana S Usenko,Alena E Kopytova,Artem D Izyumchenko,Darya G Kulabukhova,Artemiy S Silantyev,Victoria D Kazakova,Katerina S Basharova,Anastasia I Bezrukova,Luiza A Garaeva,Evgeny B Pichkur,Alexandra V Artynyuk,Irina V Miliukhina,Alla A Timofeeva,Valentina V Miroshnikova,Stanislav N Naryzhny,Anton K Emelyanov,Natalya B Zakharzhevskaya,Andrey L Konevega,Tatiana A Shtam,Sofya N Pchelina

Nature 645:793-800 PubMed40739351

2025

Programmable protein ligation on cell surfaces.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Kofoed,Girum Erkalo,Nicholas E S Tay,Xuanjia Ye,Yutong Lin,Tom W Muir
View all publications
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