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Mouse Monoclonal EPCAM antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPCAM.

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Images

Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136), expandable thumbnail
  • Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136), expandable thumbnail
  • Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (AB216136), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human EPCAM. Database link P16422

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Tested
Tested
Rhesus monkey
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

-

Predicted
Predicted

Species
Rhesus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
0.50000-2.00000 µg/mL
Notes

-

Predicted
Predicted

Species
Rhesus monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Alternative names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

Recommended products

Mouse Monoclonal EPCAM antibody. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPCAM.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human EPCAM. Database link P16422
Clone number
Cocktail EGP40/826/837/1110/1120
Purification technique
Affinity purification Protein G
Specificity

This antibody recognizes extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (Ep-CAM).

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM).

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

Associated diseases and disorders

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

Product promise

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4 product images

  • Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136), expandable thumbnail

    Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

    Overlay histogram showing MCF-7 (Human breast adenocarcinoma) cells stained with ab216136 (blue) or isotype control (red). The cells were fixed with paraformaldehyde and incubated with ab216136 at 1 ug/million cells.

  • Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136), expandable thumbnail

    Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

    Lanes 1 - 4: Merged signal (red and green). Green - ab216136 observed at 40 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

    ab216136 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EPCAM knockout sample. Wild-type and EPCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab216136 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136) at 0.5 µg/mL

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: EPCAM knockout A431 cell lysate at 20 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

    Immunohistochemical analysis of paraffin embedded, formalin fixed human breast carcinoma tissue labeling EpCAM with ab216136 at 1 μg/mL.

  • Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136), expandable thumbnail

    Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

    All lanes: Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)

    All lanes: Human lung tissue lysate

    Predicted band size: 35 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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