Rabbit Recombinant Monoclonal EPCAM antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 68 publications.
Images
![Western blot - Anti-EpCAM antibody [E144] (AB32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img166519.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EpCAM antibody [E144] (AB32392)")
![Western blot - Anti-EpCAM antibody [E144] (AB32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img175947.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EpCAM antibody [E144] (AB32392)")
![Western blot - Anti-EpCAM antibody [E144] (AB32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img100796.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EpCAM antibody [E144] (AB32392)")
![Western blot - Anti-EpCAM antibody [E144] (AB32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img86100.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EpCAM antibody [E144] (AB32392)")
Publications
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- Journal of translational medicine 21:9272023Circulating small extracellular vesicles microRNAs plus CA-125 for treatment stratification in advanced ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaofang Zhou,Mu Liu,Lijuan Sun,Yumei Cao,Shanmei Tan,Guangxia Luo,Tingting Liu,Ying Yao,Wangli Xiao,Ziqing Wan,Jie TangPubMed 38129848
- iScience 26:1080222023N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanping Wei,Jingbo Fu,Hailing Zhang,Yan Ling,Xuewu Tang,Shuowu Liu,Miao Yu,Fuyan Liu,Guokun Zhuang,Haihua Qian,Kecheng Zhang,Pinhua Yang,Xinwei Yang,Qi Yang,Shennian Ge,Baohua Zhang,Yexiong Tan,Liang Li,Hongyang WangPubMed 37954137
- Cell death & disease 14:3242023Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShakti Sagar,Md Imam Faizan,Nisha Chaudhary,Vandana Singh,Praveen Singh,Atish Gheware,Khushboo Sharma,Iqbal Azmi,Vijay Pal Singh,Gaurav Kharya,Ulaganathan Mabalirajan,Anurag Agrawal,Tanveer Ahmad,Soumya Sinha RoyPubMed 37173333
- Journal of cancer research and clinical oncology 149:8913-89222023EpCAM tumor specificity and proteoform patterns in urothelial cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranz F Dressler,Sofie Hinrichs,Marie C Roesch,Sven PernerPubMed 37154925
- Nature communications 14:1832023CD26-negative and CD26-positive tissue-resident fibroblasts contribute to functionally distinct CAF subpopulations in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia M Houthuijzen,Roebi de Bruijn,Eline van der Burg,Anne Paulien Drenth,Ellen Wientjens,Tamara Filipovic,Esme Bullock,Chiara S Brambillasca,Emilia M Pulver,Marja Nieuwland,Iris de Rink,Frank van Diepen,Sjoerd Klarenbeek,Ron Kerkhoven,Valerie G Brunton,Colinda L G J Scheele,Mirjam C Boelens,Jos JonkersPubMed 36635273
- Nature communications 13:78602022SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAya Uchida,Kenya Imaimatsu,Honoka Suzuki,Xiao Han,Hiroki Ushioda,Mami Uemura,Kasane Imura-Kishi,Ryuji Hiramatsu,Hinako M Takase,Yoshikazu Hirate,Atsuo Ogura,Masami Kanai-Azuma,Akihiko Kudo,Yoshiakira KanaiPubMed 36543770
- Cellular and molecular gastroenterology and hepatology 15:741-7642022Dysfunctional Extracellular Matrix Remodeling Supports Perianal Fistulizing Crohn's Disease by a Mechanoregulated Activation of the Epithelial-to-Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Rizzo,Federica Rubbino,Sudharshan Elangovan,Giusy Sammarco,Sara Lovisa,Silvia Restelli,Samuel Elias Pineda Chavez,Luca Massimino,Luigi Lamparelli,Marianna Paulis,Annalisa Maroli,Giulia Roda,Mohammad Shalaby,Michele Carvello,Caterina Foppa,Sheona P Drummond,Paola Spaggiari,Federica Ungaro,Antonino Spinelli,Alberto Malesci,Alessandro Repici,Anthony J Day,Alessandro Armuzzi,Silvio Danese,Stefania VetranoPubMed 36521659
- Oncology letters 24:2202022Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStéphane Chabaud,Ève Pellerin,Christophe Caneparo,Cassandra Ringuette-Goulet,Frédéric Pouliot,Stéphane BolducPubMed 35720486
- Nature 604:120-1262022Human distal airways contain a multipotent secretory cell that can regenerate alveoli.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria C Basil,Fabian L Cardenas-Diaz,Jaymin J Kathiriya,Michael P Morley,Justine Carl,Alexis N Brumwell,Jeremy Katzen,Katherine J Slovik,Apoorva Babu,Su Zhou,Madison M Kremp,Katherine B McCauley,Shanru Li,Joseph D Planer,Shah S Hussain,Xiaoming Liu,Rebecca Windmueller,Yun Ying,Kathleen M Stewart,Michelle Oyster,Jason D Christie,Joshua M Diamond,John F Engelhardt,Edward Cantu,Steven M Rowe,Darrell N Kotton,Harold A Chapman,Edward E MorriseyPubMed 35355013
- Nature cell biology 23:1240-12542021Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Zhang,Dennis K Jeppesen,James N Higginbotham,Ramona Graves-Deal,Vincent Q Trinh,Marisol A Ramirez,Yoojin Sohn,Abigail C Neininger,Nilay Taneja,Eliot T McKinley,Hiroaki Niitsu,Zheng Cao,Rachel Evans,Sarah E Glass,Kevin C Ray,William H Fissell,Salisha Hill,Kristie Lindsey Rose,Won Jae Huh,Mary Kay Washington,Gregory Daniel Ayers,Dylan T Burnette,Shivani Sharma,Leonard H Rome,Jeffrey L Franklin,Youngmin A Lee,Qi Liu,Robert J CoffeyPubMed 34887515
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-Fr | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Alternative names
CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314
Recommended products
Rabbit Recombinant Monoclonal EPCAM antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 68 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- E144
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
- Biological function summary
EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.
- Pathways
EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.
- Associated diseases and disorders
EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-EpCAM antibody [E144] (ab32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img166519.jpg "Western blot - Anti-EpCAM antibody [E144] (ab32392)")
Western blot - Anti-EpCAM antibody [E144] (ab32392)
False colour image of Western blot: Anti-EpCAM antibody [E144] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32392 was shown to bind specifically to EpCAM. A band was observed at 37/45 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in EPCAM knockout cell line ab281596 (knockout cell lysate ab282948). To generate this image, wild-type and EPCAM knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-EpCAM antibody [E144] (ab32392) at 1/1000 dilution
Lane 1: Wild-type HCT 116 cell lysate at 20 µg
Lane 2: EPCAM knockout HCT 116 cell lysate at 20 µg
Lane 3: A431 cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
Lane 5: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37-45 kDa
![Western blot - Anti-EpCAM antibody [E144] (ab32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img175947.jpg "Western blot - Anti-EpCAM antibody [E144] (ab32392)")
Western blot - Anti-EpCAM antibody [E144] (ab32392)
All lanes: Western blot - Anti-EpCAM antibody [E144] (ab32392) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Rat small intestine tissue lysate at 20 µg
Lane 3: Rat colon tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
![Western blot - Anti-EpCAM antibody [E144] (ab32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img100796.jpg "Western blot - Anti-EpCAM antibody [E144] (ab32392)")
Western blot - Anti-EpCAM antibody [E144] (ab32392)
Lanes 1 - 4: Merged signal (red and green). Green - ab32392 observed at 40 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.ab32392 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EpCAM knockout sample. Wild-type and EpCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab32392 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-EpCAM antibody [E144] (ab32392) at 1/2000 dilution
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: EPCAM knockout A431 cell lysate at 20 µg
Lane 2: Western blot - Human EPCAM knockout A-431 cell line (Human EPCAM knockout A-431 cell line ab261902)
Lane 3: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 40 kDa
![Western blot - Anti-EpCAM antibody [E144] (ab32392), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-e144-ab32392--western-blot-img86100.jpg "Western blot - Anti-EpCAM antibody [E144] (ab32392)")
Western blot - Anti-EpCAM antibody [E144] (ab32392)
All lanes: Western blot - Anti-EpCAM antibody [E144] (ab32392) at 1/2500 dilution
All lanes: A431 cell lysate
Predicted band size: 35 kDa
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