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Mouse Monoclonal EPCAM antibody. Suitable for ICC, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EPCAM aa 50-250.

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Images

Immunocytochemistry - Anti-EpCAM antibody [EGP40/1372] (AB218448), expandable thumbnail
  • Western blot - Anti-EpCAM antibody [EGP40/1372] (AB218448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (AB218448), expandable thumbnail
  • Protein Array - Anti-EpCAM antibody [EGP40/1372] (AB218448), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (AB218448), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human EPCAM aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16422

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWBProtein ArrayIHC-P
Human
Tested
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

6 products for Alternative Product

Target data

Function

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

Alternative names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

Recommended products

Mouse Monoclonal EPCAM antibody. Suitable for ICC, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EPCAM aa 50-250.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human EPCAM aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16422
Clone number
EGP40/1372
Purification technique
Affinity purification Protein A/G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

Associated diseases and disorders

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

Product promise

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7 product images

  • Immunocytochemistry - Anti-EpCAM antibody [EGP40/1372] (ab218448), expandable thumbnail

    Immunocytochemistry - Anti-EpCAM antibody [EGP40/1372] (ab218448)

    Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling EpCAM with ab218448 at 2 µg/mL (green). The nuclear counterstain is Reddot (red).

  • Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448), expandable thumbnail

    Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448)

    Lanes 1 - 4: Merged signal (red and green). Green - ab218448 observed at 35 kDa. Red - loading control, Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

    ab218448 was shown to react with EpCAM in wild-type A431 cells in western blot. Loss of signal was observed when EPCAM knockout sample was used. Wild-type and EPCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab218448 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448) at 0.5 µg/mL

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: EPCAM knockout A431 cell lysate at 20 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (ab218448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (ab218448)

    Immumohistochemical analysis of formalin fixed, paraffin embedded Human colorectal carcinoma tissue labeling EpCAM with ab218448 at 1 μg/ml.

  • Protein Array - Anti-EpCAM antibody [EGP40/1372] (ab218448), expandable thumbnail

    Protein Array - Anti-EpCAM antibody [EGP40/1372] (ab218448)

    ab218448 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (ab218448), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (ab218448)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hepatocellular Carcinoma tissue labelling EpCAM with ab218448 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

  • Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448), expandable thumbnail

    Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448)

    All lanes: Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448) at 2 µg

    All lanes: Human lung tissue lysate

    Predicted band size: 35 kDa

  • Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448), expandable thumbnail

    Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448)

    All lanes: Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448) at 1 µg/mL

    Lane 1: 293 cell line lysate

    Lane 2: A431 cell line lysate

    Lane 3: HCT116 cell line lysate

    Predicted band size: 35 kDa

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Product protocols

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