Anti-EpCAM antibody [EGP40/1372]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (AB218448)

Immumohistochemical analysis of formalin fixed, paraffin embedded Human colorectal carcinoma tissue labeling EpCAM with ab218448 at 1 μg/ml.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] (AB218448)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hepatocellular Carcinoma tissue labelling EpCAM with ab218448 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

• ICC

Supplier Data

Immunocytochemistry - Anti-EpCAM antibody [EGP40/1372] (AB218448)

Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling EpCAM with ab218448 at 2 µg/mL (green). The nuclear counterstain is Reddot (red).

• WB

Lab

Western blot - Anti-EpCAM antibody [EGP40/1372] (AB218448)

Lanes 1 - 4 : Merged signal (red and green). Green - ab218448 observed at 35 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

ab218448 was shown to react with EpCAM in wild-type A431 cells in western blot. Loss of signal was observed when EPCAM knockout sample was used. Wild-type and EPCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab218448 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448) at 0.5 µg/mL

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

EPCAM knockout A431 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

• Protein Array

Unknown

Protein Array - Anti-EpCAM antibody [EGP40/1372] (AB218448)

ab218448 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

• WB

Supplier Data

Western blot - Anti-EpCAM antibody [EGP40/1372] (AB218448)

All lanes:

Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448) at 2 µg

All lanes:

Human lung tissue lysate

Predicted band size: 35 kDa

false

• WB

Supplier Data

Western blot - Anti-EpCAM antibody [EGP40/1372] (AB218448)

All lanes:

Western blot - Anti-EpCAM antibody [EGP40/1372] (ab218448) at 1 µg/mL

Lane 1:

293 cell line lysate

Lane 2:

A431 cell line lysate

Lane 3:

HCT116 cell line lysate

Predicted band size: 35 kDa

false