Mouse Monoclonal EPCAM antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EPCAM aa 50-250.
Images
![Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--western-blot-img51756.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908)")
![Protein Array - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--protein-array-img146138.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35175.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908)")
![Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--western-blot-img82527.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (AB218908)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human EPCAM aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16422
Reactivity data
Select an application| IHC-P | WB | Protein Array | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes (Primary incubation for 30 min at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
6 products for Alternative Product
Target data
Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Alternative names
CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314
Recommended products
Mouse Monoclonal EPCAM antibody. Carrier free. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EPCAM aa 50-250.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human EPCAM aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16422
- Carrier free
- Yes
- Clone number
- EGP40/1372
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
ab218908 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
- Biological function summary
EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.
- Pathways
EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.
- Associated diseases and disorders
EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--western-blot-img51756.jpg "Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)")
Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)
This data was developed using the same antibody clone in a different buffer formulation (Anti-EpCAM antibody [EGP40/1372] ab218448).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-EpCAM antibody [EGP40/1372] ab218448 observed at 35 kDa. Red - loading control, Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
Anti-EpCAM antibody [EGP40/1372] ab218448 was shown to react with EpCAM in A431 wild-type cells in western blot. Loss of signal was observed when EPCAM knockout sample was used. A431 wild-type and EPCAM knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-EpCAM antibody [EGP40/1372] ab218448 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-EpCAM antibody [EGP40/1372] (Anti-EpCAM antibody [EGP40/1372] ab218448) at 0.5 µg/mL
Lane 1: Wild-type A431 cell lysate at 20 µg
Lane 2: EPCAM knockout A431 cell lysate at 20 µg
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 35 kDa
![Protein Array - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--protein-array-img146138.jpg "Protein Array - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)")
Protein Array - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)
This data was produced with Anti-EpCAM antibody [EGP40/1372] ab218448, the same antibody in a different formulation with BSA and Azide.
Anti-EpCAM antibody [EGP40/1372] ab218448 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img35175.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)
Immumohistochemical analysis of formalin-fixed, paraffin-embedded human colorectal carcinoma tissue labeling EpCAM with ab218908 at 1 μg/ml.
![Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-egp40-1372-bsa-and-azide-free-ab218908--western-blot-img82527.jpg "Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)")
Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908)
All lanes: Western blot - Anti-EpCAM antibody [EGP40/1372] - BSA and Azide free (ab218908) at 1 µg/mL
Lane 1: 293 cell lysate
Lane 2: A431 cell lysate
Lane 3: HCT116 cell lysate
Predicted band size: 35 kDa
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