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AB232437

Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EPCAM antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CD326, Tacstd1, Epcam, Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, Protein 289A, Tumor-associated calcium signal transducer 1, EGP314, mEGP314

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling EpCAM with ab213501 at 1/2000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse lung is observed (PMID : 15637741). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213501).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling EpCAM with ab213501 at 1/2000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse colon is observed (PMID : 15637741). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213501).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling EpCAM with ab213501 at 1/2000 dilution, followed by Goat anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on rat colon is observed (PMID : 15637741). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213501).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 4T1 (mouse mammary gland carcinoma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling EpCAM with ab213501 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on 4T1 cells.

Negative control : NIH/3T3 (PMID : 23264216).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213501).

Immunoprecipitation - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)
  • IP

Lab

Immunoprecipitation - Anti-EpCAM antibody [EPR20533-266] - BSA and Azide free (AB232437)

EpCAM was immunoprecipitated from 0.35 mg of 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate with ab213501 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab213501 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab13136), was used as secondary antibody at 1/10000 dilution.

Lane 1 : 4T1 whole cell lysate 10 µg (Input).

Lane 2 : ab213501 IP in 4T1 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213501 in 4T1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213501).

All lanes:

Immunoprecipitation - Anti-EpCAM antibody [EPR20533-266] (<a href='/en-us/products/primary-antibodies/epcam-antibody-epr20533-266-ab213501'>ab213501</a>)

Predicted band size: 35 kDa

Observed band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20533-266

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IHC-P, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab232437 is the carrier-free version of ab213501.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
See full target information Epcam
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of medicinal chemistry 67:19282-19303 PubMed39450890

2024

Discovery and Characterization of BAY-184: A New Potent and Selective Acylsulfonamide-Benzofuran -Active KAT6AB Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Antonius Ter Laak,Roman C Hillig,Steven J Ferrara,Daniel Korr,Naomi Barak,Philip Lienau,Simon Herbert,Amaury Ernesto Fernández-Montalván,Roland Neuhaus,Mátyás Gorjánácz,Vera Puetter,Volker Badock,Wilhelm Bone,Craig Strathdee,Franziska Siegel,Christoph Schatz,Katrin Nowak-Reppel,Olaf Doehr,Stefan Gradl,Ingo V Hartung,Matthew Meyerson,Léa Bouché
View all publications
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