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Rabbit Recombinant Monoclonal EPCAM antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-P and reacts with Mouse samples.


Images

Flow Cytometry - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (AB228876), expandable thumbnail
  • Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (AB228876), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (AB228876), expandable thumbnail
  • Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (AB228876), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (AB228876), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Mouse
Tested
Tested
Expected
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Version

6 products for Alternative Product

Target data

Function

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

Alternative names

Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, Protein 289A, Tumor-associated calcium signal transducer 1, EGP314, mEGP314, Epcam, Tacstd1

Recommended products

Rabbit Recombinant Monoclonal EPCAM antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-P and reacts with Mouse samples.

Alternative names

Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, Protein 289A, Tumor-associated calcium signal transducer 1, EGP314, mEGP314, Epcam, Tacstd1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR20533-63

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab228876 is the carrier-free version of Anti-EpCAM antibody [EPR20533-63] ab221552.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

Associated diseases and disorders

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876), expandable thumbnail

    Flow Cytometry - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876)

    Flow cytometric analysis of 4% paraformaldehyde-fixed 4T1 (mouse mammary gland carcinoma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling EpCAM with Anti-EpCAM antibody [EPR20533-63] ab221552 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    Flow cytometry was performed with fresh cells without fixation and permeabilization.

    Negative control: NIH/3T3 (PMID:23264216).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EpCAM antibody [EPR20533-63] ab221552).

  • Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876), expandable thumbnail

    Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876)

    EpCAM was immunoprecipitated from 0.35 mg of mouse kidney lysate with Anti-EpCAM antibody [EPR20533-63] ab221552 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-EpCAM antibody [EPR20533-63] ab221552 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse kidney lysate 10 μg (Input).

    Lane 2: Anti-EpCAM antibody [EPR20533-63] ab221552 IP in mouse kidney lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-EpCAM antibody [EPR20533-63] ab221552 in mouse kidney lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EpCAM antibody [EPR20533-63] ab221552).

    All lanes: Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] (Anti-EpCAM antibody [EPR20533-63] ab221552)

    Predicted band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 4T1 (mouse mammary gland carcinoma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling EpCAM with Anti-EpCAM antibody [EPR20533-63] ab221552  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining on 4T1 cells.

    Negative control: NIH/3T3 (PMID:23264216).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EpCAM antibody [EPR20533-63] ab221552).

  • Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876), expandable thumbnail

    Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876)

    EpCAM was immunoprecipitated from 0.35 mg of 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate with Anti-EpCAM antibody [EPR20533-63] ab221552 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-EpCAM antibody [EPR20533-63] ab221552 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: 4T1 (Mouse mammary gland carcinoma) whole cell lysate 10 μg (Input).

    Lane 2: Anti-EpCAM antibody [EPR20533-63] ab221552 IP in 4T1 whole cell lysate .

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-EpCAM antibody [EPR20533-63] ab221552 in 4T1 whole cell lysate .

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    The band between 12-15kDa has been documented in literature as the cleaved form of EpCAM (PMID: 23409978).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EpCAM antibody [EPR20533-63] ab221552).

    All lanes: Immunoprecipitation - Anti-EpCAM antibody [EPR20533-63] (Anti-EpCAM antibody [EPR20533-63] ab221552)

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling EpCAM with Anti-EpCAM antibody [EPR20533-63] ab221552 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse lung is observed (PMID: 15637741). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EpCAM antibody [EPR20533-63] ab221552).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] - BSA and Azide free (ab228876)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling EpCAM with Anti-EpCAM antibody [EPR20533-63] ab221552 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse colon is observed (PMID: 15637741). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EpCAM antibody [EPR20533-63] ab221552).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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