AB124825

Anti-EpCAM antibody [EPR677(2)]

Name: Anti-EpCAM antibody [EPR677(2)] (ab124825) | Abcam
Brand: Abcam Limited
SKU: AB124825
Price: 200 USD
Availability: InStock
Rating: 1 (1 reviews)

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(12 Publications)

Rabbit Recombinant Monoclonal EPCAM antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human endometrial adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Tissue Microarrays stained for "Anti-EpCAM antibody [EPR677(2)]" using "ab124825"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab124825 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Unknown

Western blot - Anti-EpCAM antibody [EPR677(2)] (AB124825)

All lanes:

Western blot - Anti-EpCAM antibody [EPR677(2)] (ab124825) at 1/1000 dilution

Lane 1:

HCT116 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

HT29 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 35 kDa,37 kDa,44 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825 showing negative staining in Glioma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825 showing positive staining in Normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825 showing positive staining in Urinary bladder transitional carcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

ab124825 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-EpCAM antibody [EPR677(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-EpCAM antibody [EPR677(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-EpCAM antibody [EPR677(2)]
Biotin

Biotin Anti-EpCAM antibody [EPR677(2)]
HRP

HRP Anti-EpCAM antibody [EPR677(2)]
Carrier free

Anti-EpCAM antibody [EPR677(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR677(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Western blots of mouse and rat samples are negative." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

See full target information EPCAM

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Asian Pacific journal of cancer prevention : APJCP 24:3247-3259 PubMed37774079

2023

Clinicopathologic Impact of NANOG, ZEB1, and EpCAM Biomarkers on Prognosis of Serous Ovarian Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hanaa M Ibrahim,Aziza E Abdelrahman,Eman Elsebai,Shimaa A Gharieb,Moamna M Fahmy,Mohamed S H Ramadan,Mohamed A Wasfy,Asmaa Abdullatif

World journal of surgical oncology 20:15 PubMed35016698

2022

Significant co-expression of putative cancer stem cell markers, EpCAM and CD166, correlates with tumor stage and invasive behavior in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Elham Kalantari,Tahereh Taheri,Saba Fata,Maryam Abolhasani,Mitra Mehrazma,Zahra Madjd,Mojgan Asgari

Developmental cell 52:413-428.e6 PubMed32097652

2020

Single-Chromosomal Gains Can Function as Metastasis Suppressors and Promoters in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anand Vasudevan,Prasamit S Baruah,Joan C Smith,Zihua Wang,Nicole M Sayles,Peter Andrews,Jude Kendall,Justin Leu,Narendra Kumar Chunduri,Dan Levy,Michael Wigler,Zuzana Storchová,Jason M Sheltzer

Journal of cellular biochemistry 121:3941-3951 PubMed31696964

2019

Ring1 promotes the transformation of hepatic progenitor cells into cancer stem cells through the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhu,Jiangwei Li,Jun Li,Jin Sun,Ying Guo,Hongwei Tian,Liang Li,Chen Zhang,Mengjiao Shi,Guangyao Kong,Zongfang Li

BMC cancer 19:831 PubMed31443698

2019

Prognostic value of biomarkers EpCAM and αB-crystallin associated with lymphatic metastasis in breast cancer by iTRAQ analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zeng,Xiyun Deng,Jingmin Zhong,Li Yuan,Xiaojun Tao,Sai Zhang,Yong Zeng,Guangchun He,Pingping Tan,Yongguang Tao

Cytopathology : official journal of the British So 29:247-253 PubMed29280203

2017

Improving the cytological diagnosis of high-grade serous carcinoma in ascites with a panel of complementary biomarkers in cell blocks.

Applications

IHC-P

Species

Human

X Zhang,L Chen,Y Liu,Y Xu,X Zhang,Y Shi,C Wang,P L Zhang,Y Liu

Breast cancer research : BCR 19:102 PubMed28865492

2017

Pathobiology of the 129:Stat1 mouse model of human age-related ER-positive breast cancer with an immune infiltrate-excluded phenotype.

Applications

IHC

Species

Unspecified reactive species

Hidetoshi Mori,Jane Q Chen,Robert D Cardiff,Zsófia Pénzváltó,Neil E Hubbard,Louis Schuetter,Russell C Hovey,Josephine F Trott,Alexander D Borowsky

Scientific reports 7:5575 PubMed28717241

2017

Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Bryony V Natale,Christina Schweitzer,Martha Hughes,Maria A Globisch,Ramie Kotadia,Emilie Tremblay,Priscilla Vu,James C Cross,David R C Natale

Journal of comparative pathology 155:299-304 PubMed27567927

2016

Epithelial Cell Adhesion Molecule Expression in Canine Tumours.

Applications

Species

Human

D H Thamm,D F Hayes,T Meuten,T Laver,D G Thomas

International journal of cancer 137:991-8 PubMed25622566

2015

Analysis of circulating tumor cells derived from advanced gastric cancer.

Applications

IHC-P

Species

Human

Kosei Toyoshima,Akira Hayashi,Masahide Kashiwagi,Naoko Hayashi,Masaaki Iwatsuki,Takatsugu Ishimoto,Yoshifumi Baba,Hideo Baba,Yoshikazu Ohta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EpCAM antibody [EPR677(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Western blot - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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