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AB46714

Anti-EpCAM antibody [HEA125], prediluted

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(4 Publications)

Mouse Monoclonal EPCAM antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714)

ICC/IF image of ab46714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46714 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HEA125

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.3 Preservative: 0.09% Sodium azide Constituents: BSA, PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
See full target information EPCAM
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

ImmunoHorizons 8:500-510 PubMed39018546

2024

Comprehensive Flow Cytometric, Immunohistologic, and Molecular Assessment of Thymus Function in Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Laura P Hale,Andrew N Macintyre,Dawn E Bowles,Jean Kwun,Jie Li,Barbara Theriot,Joseph W Turek

Bio-protocol 8:e3124 PubMed34532562

2018

Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Vikash Kumar,Asok Mukhopadhyay

Nature communications 9:894 PubMed29497050

2018

Whole-exome sequencing reveals the origin and evolution of hepato-cholangiocarcinoma.

Applications

IHC-P

Species

Human

Anqiang Wang,Liangcai Wu,Jianzhen Lin,Longzhe Han,Jin Bian,Yan Wu,Simon C Robson,Lai Xue,Yunxia Ge,Xinting Sang,Wenze Wang,Haitao Zhao

World journal of hepatology 5:425-32 PubMed24023981

2013

Participation of peribiliary glands in biliary tract pathophysiologies.

Applications

Unspecified application

Species

Unspecified reactive species

Saya Igarashi,Yasunori Sato,Xiang Shan Ren,Kenichi Harada,Motoko Sasaki,Yasuni Nakanuma
View all publications
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Product promise

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