Mouse Monoclonal EPCAM antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-hea125-prediluted-ab46714--immunocytochemistry-immunofluorescence-img111875.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714)")
Publications
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- Bio-protocol 8:e31242018Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVikash Kumar,Asok MukhopadhyayPubMed 34532562
- Nature communications 9:8942018Whole-exome sequencing reveals the origin and evolution of hepato-cholangiocarcinoma.Applications:IHC-PReactive species:HumanAnqiang Wang,Liangcai Wu,Jianzhen Lin,Longzhe Han,Jin Bian,Yan Wu,Simon C Robson,Lai Xue,Yunxia Ge,Xinting Sang,Wenze Wang,Haitao ZhaoPubMed 29497050
- World journal of hepatology 5:425-322013Participation of peribiliary glands in biliary tract pathophysiologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaya Igarashi,Yasunori Sato,Xiang Shan Ren,Kenichi Harada,Motoko Sasaki,Yasuni NakanumaPubMed 24023981
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: BSA, PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16422
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Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Version
Target data
Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Alternative names
CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314
Recommended products
Mouse Monoclonal EPCAM antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: BSA, PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information. Database link P16422
- Clone number
- HEA125
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
- Biological function summary
EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.
- Pathways
EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.
- Associated diseases and disorders
EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (ab46714), expandable thumbnail](https://content.abcam.com/products/images/epcam-antibody-hea125-prediluted-ab46714--immunocytochemistry-immunofluorescence-img111875.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (ab46714)")
Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (ab46714)
ICC/IF image of ab46714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46714 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (Donkey Anti-Goat IgG H&L (DyLight® 488) ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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