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AB300565

Anti-EPCR/CD201 antibody [EPR25647-49]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(1 Publication)

Anti-EPCR/CD201 antibody [EPR25647-49] is a Rabbit Monoclonal antibody that is used in EPCR/CD201 IHC-P, Western Blot. Suitable for Human, Mouse samples.

Endothelial Protein C Receptor (EPCR), also known as CD201, plays a significant role in tumor progression by enhancing cell survival, proliferation, and migration. EPCR binds to APC, which then activates PAR-1. This activation leads to anti-inflammatory and cytoprotective effects that can create a favorable environment for tumor cells to thrive. EPCR is overexpressed in several cancers including epithelial cancer and is a ligand for gamma-delta T cells. EPCR promotes breast cancer invasion and 3D growth.

View Alternative Names

CD201, EPCR, PROCR, Endothelial protein C receptor, Activated protein C receptor, Endothelial cell protein C receptor, APC receptor

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling EPCR/CD201 with ab300565 at 1/2000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon cancer (PMID : 12110501). The section was incubated with ab300565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EPCR/CD201 with ab300565 at 1/2000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on endothelial cells of human kidney (PMID : 26990852). The section was incubated with ab300565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EPCR/CD201 with ab300565 at 1/2000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human placenta (PMID : 32003123). The section was incubated with ab300565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • WB

Lab

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Exposure time : ab300525 : 180 seconds; ab300565 : 5 seconds

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

The band beneath the target band is supposed to be a soluble form (PMID : 19806250).

ab300525 is less sensitive than ab300565. ab300525 needs a high sensitivity ECL substrate.

Lanes 1 - 15:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-5] (<a href='/en-us/products/primary-antibodies/epcr-cd201-antibody-epr25647-5-ab300525'>ab300525</a>) at 1/1000 dilution

Lanes 11 - 15:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lanes 1, 6 and 11:

Human lung tissue lysate at 20 µg

Lanes 2, 7 and 12:

HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lanes 3, 8 and 13:

Ea.Hy926 ( human somatic cell hybrid endothelial) whole cell lysate at 20 µg

Lanes 4, 9 and 14:

RKO (human colorectal carcinoma cell) whole cell lysate at 20 µg

Lanes 5, 10 and 15:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa

false

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The band beneath the target band is supposed to be a soluble form. (PMID : 19806250)

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate

Lane 2:

Human lu tissue lysate 20

Lane 3:

Human skin tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Blocking and diluting buffer and concentration : 5% NFDM/TBST PROCR is a glycoprotein of approximately 45 kDa and detected as a 23kDa band after treated with Protein Deglycosylation MIX II.

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1:

Untreated HUVEC whole cell lysate

Lane 2:

HUVEC whole cell lysate treated with Protein Deglycosylation Mix II

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,45 kDa

false

Exposure time: 26s

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Jurkat (PMID : 7929370) The band beneath the target band is supposed to be a soluble form. (PMID : 19806250)

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell), whole cell lysate

Lane 2:

Ea.Hy926 (human somatic cell hybrid endothelial), whole cell lysate

Lane 3:

RKO (human colon epithelial cell), whole cell lysate

Lane 4:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 15s

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : B16-F10 (PMID : 19188668)

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1:

bEnd.3 (mouse brain endothelioma), whole cell lysate 20 µg

Lane 2:

B16-F10 (mouse skin melanoma mixture of spindle-shaped and epithelial-like), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25647-49

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPCR also known as CD201 stands for Endothelial Protein C Receptor. It is a glycoprotein with a molecular mass of approximately 46 kDa. EPCR is expressed mainly on endothelial cells which line the interior surface of blood vessels. This receptor plays a mechanical role in binding to protein C a zymogen that is converted to activated protein C (APC) by thrombin-thrombomodulin complex. By facilitating the activation of protein C EPCR contributes to the regulation of blood coagulation and inflammation processes.
Biological function summary

The EPCR is part of the protein C anticoagulant pathway where it acts as a cofactor. EPCR is vital in enhancing the activation of protein C on the endothelial surface reducing coagulation activity. It interacts closely with thrombomodulin and protein C to form this complex preventing excessive thrombus formation and contributing to vascular integrity. Apart from its anticoagulant role EPCR also modulates inflammatory responses supporting its function as a bridge between coagulation and inflammation.

Pathways

EPCR lies within the protein C anticoagulation pathway and the endothelial signaling pathways. Its primary partner in the anticoagulation pathway is APC which degrades activated factors Va and VIIIa providing a check against coagulation. Additionally EPCR’s interaction with APC and thrombomodulin links this protein to the endothelial signaling pathway where it affects cell survival and inflammation regulation. This interaction is essential to maintain hemostatic balance and control vascular responses to inflammatory stimuli.

EPCR associates with conditions like thrombosis and sepsis. In thrombosis inadequate expression or function of EPCR can lead to uncontrolled blood clotting due to insufficient activation of protein C. Sepsis a severe inflammatory response to infection shows abnormal modulation of the protein C pathway where EPCR's role becomes pivotal. Additionally APC which interacts directly with EPCR is critical in the pathological states of these diseases highlighting the therapeutic potential in targeting EPCR pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
See full target information PROCR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1488552 PubMed39902050

2025

The generation and evaluation of TKO/hCD55/hTM/hEPCR gene-modified pigs for clinical organ xenotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Guoli Huai,Yong Wang,Jiaxiang Du,Zhenhui Cheng,Yuxuan Xie,Jia Zhou,Hongmei Tang,Yanyan Jiang,Xiangyang Xing,Shaoping Deng,Dengke Pan
View all publications
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Product promise

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