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AB300566

Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free)

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Rabbit Recombinant Monoclonal EPCR/CD201 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse samples.

View Alternative Names

CD201, EPCR, PROCR, Endothelial protein C receptor, Activated protein C receptor, Endothelial cell protein C receptor, APC receptor

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling EPCR/CD201 with ab300565 at 1/2000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon cancer (PMID : 12110501). The section was incubated with ab300565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling EPCR/CD201 with ab300565 at 1/2000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on endothelial cells of human kidney (PMID : 26990852). The section was incubated with ab300565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EPCR/CD201 with ab300565 at 1/2000 (0.524 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human placenta (PMID : 32003123). The section was incubated with ab300565 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation. Negative control : Jurkat (PMID : 7929370) The band beneath the target band is supposed to be a soluble form. (PMID : 19806250) Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (<a href='/en-us/products/primary-antibodies/epcr-cd201-antibody-epr25647-49-ab300565'>ab300565</a>) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell), whole cell lysate

Lane 2:

Ea.Hy926 (human somatic cell hybrid endothelial), whole cell lysate

Lane 3:

RKO (human colon epithelial cell), whole cell lysate

Lane 4:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 15s

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation. The band beneath the target band is supposed to be a soluble form. (PMID : 19806250) Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (<a href='/en-us/products/primary-antibodies/epcr-cd201-antibody-epr25647-49-ab300565'>ab300565</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate

Lane 2:

Human lu tissue lysate 20

Lane 3:

Human skin tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation. PROCR is a glycoprotein of approximately 45 kDa and detected as a 23kDa band after treated with Protein Deglycosylation MIX II. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (<a href='/en-us/products/primary-antibodies/epcr-cd201-antibody-epr25647-49-ab300565'>ab300565</a>) at 1/1000 dilution

Lane 1:

Untreated HUVEC whole cell lysate

Lane 2:

HUVEC whole cell lysate treated with Protein Deglycosylation Mix II

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,45 kDa

false

Exposure time: 26s

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)
  • WB

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation. Negative control : B16-F10 (PMID : 19188668) Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (<a href='/en-us/products/primary-antibodies/epcr-cd201-antibody-epr25647-49-ab300565'>ab300565</a>) at 1/1000 dilution

Lane 1:

bEnd.3 (mouse brain endothelioma), whole cell lysate 20 µg

Lane 2:

B16-F10 (mouse skin melanoma mixture of spindle-shaped and epithelial-like), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 45 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25647-49

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPCR also known as CD201 stands for Endothelial Protein C Receptor. It is a glycoprotein with a molecular mass of approximately 46 kDa. EPCR is expressed mainly on endothelial cells which line the interior surface of blood vessels. This receptor plays a mechanical role in binding to protein C a zymogen that is converted to activated protein C (APC) by thrombin-thrombomodulin complex. By facilitating the activation of protein C EPCR contributes to the regulation of blood coagulation and inflammation processes.
Biological function summary

The EPCR is part of the protein C anticoagulant pathway where it acts as a cofactor. EPCR is vital in enhancing the activation of protein C on the endothelial surface reducing coagulation activity. It interacts closely with thrombomodulin and protein C to form this complex preventing excessive thrombus formation and contributing to vascular integrity. Apart from its anticoagulant role EPCR also modulates inflammatory responses supporting its function as a bridge between coagulation and inflammation.

Pathways

EPCR lies within the protein C anticoagulation pathway and the endothelial signaling pathways. Its primary partner in the anticoagulation pathway is APC which degrades activated factors Va and VIIIa providing a check against coagulation. Additionally EPCR’s interaction with APC and thrombomodulin links this protein to the endothelial signaling pathway where it affects cell survival and inflammation regulation. This interaction is essential to maintain hemostatic balance and control vascular responses to inflammatory stimuli.

EPCR associates with conditions like thrombosis and sepsis. In thrombosis inadequate expression or function of EPCR can lead to uncontrolled blood clotting due to insufficient activation of protein C. Sepsis a severe inflammatory response to infection shows abnormal modulation of the protein C pathway where EPCR's role becomes pivotal. Additionally APC which interacts directly with EPCR is critical in the pathological states of these diseases highlighting the therapeutic potential in targeting EPCR pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
See full target information PROCR
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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