AB236517

Anti-EPCR/CD201 antibody [OTI12H5]

Name: Anti-EPCR/CD201 antibody [OTI12H5] (ab236517) | Abcam
Brand: Abcam Limited
SKU: AB236517

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(1 Publication)

Mouse Monoclonal EPCR/CD201 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PROCR aa 1 to C-terminus.

View Alternative Names

CD201, EPCR, PROCR, Endothelial protein C receptor, Activated protein C receptor, Endothelial cell protein C receptor, APC receptor

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Paraffin-embedded human endometrium tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Paraffin-embedded human lung tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Paraffin-embedded human colon adenocarcinoma tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Paraffin-embedded human breast tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Paraffin-embedded human colon tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Paraffin-embedded human prostate tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Paraffin-embedded human bladder tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

All lanes:

Western blot - Anti-EPCR/CD201 antibody [OTI12H5] (ab236517) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY EPCR/CD201 cDNA transfected HEK-293T cell lysate at 5 µg

Predicted band size: 27 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI12H5

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PROCR aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UNN8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPCR also known as CD201 stands for Endothelial Protein C Receptor. It is a glycoprotein with a molecular mass of approximately 46 kDa. EPCR is expressed mainly on endothelial cells which line the interior surface of blood vessels. This receptor plays a mechanical role in binding to protein C a zymogen that is converted to activated protein C (APC) by thrombin-thrombomodulin complex. By facilitating the activation of protein C EPCR contributes to the regulation of blood coagulation and inflammation processes.

Biological function summary

The EPCR is part of the protein C anticoagulant pathway where it acts as a cofactor. EPCR is vital in enhancing the activation of protein C on the endothelial surface reducing coagulation activity. It interacts closely with thrombomodulin and protein C to form this complex preventing excessive thrombus formation and contributing to vascular integrity. Apart from its anticoagulant role EPCR also modulates inflammatory responses supporting its function as a bridge between coagulation and inflammation.

Pathways

EPCR lies within the protein C anticoagulation pathway and the endothelial signaling pathways. Its primary partner in the anticoagulation pathway is APC which degrades activated factors Va and VIIIa providing a check against coagulation. Additionally EPCR’s interaction with APC and thrombomodulin links this protein to the endothelial signaling pathway where it affects cell survival and inflammation regulation. This interaction is essential to maintain hemostatic balance and control vascular responses to inflammatory stimuli.

EPCR associates with conditions like thrombosis and sepsis. In thrombosis inadequate expression or function of EPCR can lead to uncontrolled blood clotting due to insufficient activation of protein C. Sepsis a severe inflammatory response to infection shows abnormal modulation of the protein C pathway where EPCR's role becomes pivotal. Additionally APC which interacts directly with EPCR is critical in the pathological states of these diseases highlighting the therapeutic potential in targeting EPCR pathway.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

See full target information PROCR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal oncology 14:1669-1693 PubMed37720449

2023

Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Ruijiang Lin,Minjie Ma,Biao Han,Ya Zheng,Yuping Wang,Yongning Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPCR/CD201 antibody [OTI12H5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Western blot - Anti-EPCR/CD201 antibody [OTI12H5] (AB236517)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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