AB108600

Anti-EPF antibody [EPR4476]

Name: Anti-EPF antibody [EPR4476] (ab108600) | Abcam
Brand: Abcam Limited
SKU: AB108600
Price: 5470 USD
Availability: InStock
Rating: 4 (5 reviews)

RabMAb
Recombinant
What is this?

(5 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal EPF antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications.

View Alternative Names

Hsp10, 10 kDa chaperonin, Chaperonin 10, Early-pregnancy factor, Heat shock protein family E member 1, CPN10, EPF, HSPE1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemical staining of EPF in paraffin embedded Human colon tissue using ab108600 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemical staining of EPF in paraffin embedded Human kidney tissue using ab108600 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-EPF antibody [EPR4476] (AB108600)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPF antibody [EPR4476] (AB108600)

Immunofluorescent staining of EPF in HeLa cells using ab108600 at a dilution of 1/100.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

ab108600 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

ab108600 showing positive staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

ab108600 showing positive staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-EPF antibody [EPR4476] (AB108600)

All lanes:

Western blot - Anti-EPF antibody [EPR4476] (ab108600) at 1/10000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

Neuro-2a cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 10 kDa

Observed band size: 11 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

ab108600 showing positive staining in Normal stomach tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

ab108600 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-EPF antibody [EPR4476]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-EPF antibody [EPR4476]
Carrier free

Anti-EPF antibody [EPR4476] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4476

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPF also known as Embryo Paracrine Factor is a protein with a mass of approximately 18-20 kDa. This protein primarily expresses in embryonic tissues and certain adult tissues with regeneration and repair abilities. EPF functions as a signaling molecule and plays a role in cellular communication influencing different cellular behaviors like proliferation differentiation and migration.

Biological function summary

EPF impacts multiple cellular processes during embryogenesis and tissue regeneration. It does not function as a part of a larger protein complex but interacts directly with surface receptors to initiate signal transduction pathways. EPF activities help in regulating cell cycle transitions balancing cell growth with apoptosis for proper tissue formation and healing. By mediating these activities EPF is critical for normal development and efficient repair processes.

Pathways

Several molecular networks engage with EPF to exert its influence. One of the primary pathways involving EPF is the Wnt signaling pathway which contributes to the regulation of gene expression cellular growth and differentiation. Through this pathway EPF interacts with proteins like β-catenin. Additionally EPF also associates with the PI3K/Akt pathway which further highlights its involvement in controlling cellular survival and growth dynamics.

Abnormal EPF function is linked with cancer progression and regenerative disorders. Dysregulation of EPF can lead to uncontrolled cell proliferation and has been observed in certain cancers such as colorectal cancer. Furthermore impaired EPF signaling might affect wound healing processes leading to chronic wounds. During these disease processes EPF interacts with other important signaling molecules such as p53 in cancer to influence disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed : 11422376, PubMed : 1346131, PubMed : 7912672). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).

See full target information HSPE1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

American journal of men's health 18:15579883241306904 PubMed39688244

2024

HSPE1 Inhibits Bladder Cancer Ferroptosis via a Glutathione-Dependent Mechanism by Suppressing GPX4.

Applications

Unspecified application

Species

Unspecified reactive species

Jiqiang Cheng,Lina Wang,Wenlong Wang,Hao Liang,Shuaishuai Shan,Shaopeng Zhang,Zekun Wang,Qingbing Wang

Discover. Oncology 14:107 PubMed37351671

2023

HSF1 is a novel prognostic biomarker in high-risk prostate cancer that correlates with ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

GaoZhen Jia,WenBo Wu,Lei Chen,Yang Yu,QiLin Tang,HaiTao Liu,Qi Jiang,BangMin Han

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP 30:1321-1325 PubMed33397061

2021

Tissue Nuclear Matrix Protein Expression 22 in Various Grades and Stages of Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Quratulain Mati,Samina Qamar,Sobia Ashraf,Muhammad Abbas Khokhar,Usma Arshad

Signal transduction and targeted therapy 4:40 PubMed31637018

2019

Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Song,Ji Ma,Zhixuan Bian,Shuhua Chen,Jiabei Zhu,Jing Wang,Nan Huang,Minzhi Yin,Fenyong Sun,Min Xu,Qiuhui Pan

Cell metabolism 30:1040-1054.e7 PubMed31523008

2019

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.

Applications

Unspecified application

Species

Unspecified reactive species

Saara Forsström,Christopher B Jackson,Christopher J Carroll,Mervi Kuronen,Eija Pirinen,Swagat Pradhan,Anastasiia Marmyleva,Mari Auranen,Iida-Marja Kleine,Nahid A Khan,Anne Roivainen,Päivi Marjamäki,Heidi Liljenbäck,Liya Wang,Brendan J Battersby,Uwe Richter,Vidya Velagapudi,Joni Nikkanen,Liliya Euro,Anu Suomalainen

Archivum immunologiae et therapiae experimentalis 67:401-414 PubMed31324924

2019

Mitochondrial Heat Shock Response Induced by Ectromelia Virus is Accompanied by Reduced Apoptotic Potential in Murine L929 Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zbigniew Wyżewski,Karolina P Gregorczyk-Zboroch,Matylda B Mielcarska,Magdalena Bossowska-Nowicka,Justyna Struzik,Joanna Szczepanowska,Felix N Toka,Marek G Niemiałtowski,Lidia Szulc-Dąbrowska

Cell death & disease 10:147 PubMed30770797

2019

Fhit-Fdxr interaction in the mitochondria: modulation of reactive oxygen species generation and apoptosis in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Druck,Douglas G Cheung,Dongju Park,Francesco Trapasso,Flavia Pichiorri,Marco Gaspari,Tiziana Palumbo,Rami I Aqeilan,Eugenio Gaudio,Hiroshi Okumura,Rodolfo Iuliano,Cinzia Raso,Kari Green,Kay Huebner,Carlo M Croce

Oncotarget 9:30731-30747 PubMed30112103

2018

Systematic verification of bladder cancer-associated tissue protein biomarker candidates in clinical urine specimens.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Han Tsai,Yi-Ting Chen,Ying-Hsu Chang,Chuen Hsueh,Chung-Yi Liu,Yu-Sun Chang,Chien-Lun Chen,Jau-Song Yu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 28:1666-81 PubMed24378874

2014

Neuron-specific expression of CuZnSOD prevents the loss of muscle mass and function that occurs in homozygous CuZnSOD-knockout mice.

Applications

Species

Mouse

Giorgos K Sakellariou,Carol S Davis,Yun Shi,Maxim V Ivannikov,Yiqiang Zhang,Aphrodite Vasilaki,Gregory T Macleod,Arlan Richardson,Holly Van Remmen,Malcolm J Jackson,Anne McArdle,Susan V Brooks

Human molecular genetics 22:4871-87 PubMed23851121

2013

Loss of mitochondrial peptidase Clpp leads to infertility, hearing loss plus growth retardation via accumulation of CLPX, mtDNA and inflammatory factors.

Applications

Unspecified application

Species

Unspecified reactive species

Suzana Gispert,Dajana Parganlija,Michael Klinkenberg,Stefan Dröse,Ilka Wittig,Michel Mittelbronn,Pawel Grzmil,Sebastian Koob,Andrea Hamann,Michael Walter,Finja Büchel,Thure Adler,Martin Hrabé de Angelis,Dirk H Busch,Andreas Zell,Andreas S Reichert,Ulrich Brandt,Heinz D Osiewacz,Marina Jendrach,Georg Auburger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPF antibody [EPR4476]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Immunocytochemistry/ Immunofluorescence - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Western blot - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] (AB108600)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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