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AB244229

Anti-EPF antibody [EPR4476] - BSA and Azide free

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Rabbit Recombinant Monoclonal EPF antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

Hsp10, 10 kDa chaperonin, Chaperonin 10, Early-pregnancy factor, Heat shock protein family E member 1, CPN10, EPF, HSPE1

9 Images
Western blot - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • WB

Unknown

Western blot - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EPF antibody [EPR4476] (<a href='/en-us/products/primary-antibodies/epf-antibody-epr4476-ab108600'>ab108600</a>) at 1/10000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

Neuro-2a cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 10 kDa

Observed band size: 11 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

ab108600 showing positive staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

ab108600 showing positive staining in Normal stomach tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of EPF in paraffin embedded Human colon tissue, using ab108600 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

ab108600 showing positive staining in Normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

ab108600 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of EPF in paraffin embedded Human kidney tissue, using ab108600 at a dilution of 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of EPF in HeLa cells, using ab108600 at a dilution of 1/100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPF antibody [EPR4476] - BSA and Azide free (AB244229)

This data was developed using ab108600, the same antibody clone in a different buffer formulation.

ab108600 showing positive staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4476

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPF also known as Embryo Paracrine Factor is a protein with a mass of approximately 18-20 kDa. This protein primarily expresses in embryonic tissues and certain adult tissues with regeneration and repair abilities. EPF functions as a signaling molecule and plays a role in cellular communication influencing different cellular behaviors like proliferation differentiation and migration.
Biological function summary

EPF impacts multiple cellular processes during embryogenesis and tissue regeneration. It does not function as a part of a larger protein complex but interacts directly with surface receptors to initiate signal transduction pathways. EPF activities help in regulating cell cycle transitions balancing cell growth with apoptosis for proper tissue formation and healing. By mediating these activities EPF is critical for normal development and efficient repair processes.

Pathways

Several molecular networks engage with EPF to exert its influence. One of the primary pathways involving EPF is the Wnt signaling pathway which contributes to the regulation of gene expression cellular growth and differentiation. Through this pathway EPF interacts with proteins like β-catenin. Additionally EPF also associates with the PI3K/Akt pathway which further highlights its involvement in controlling cellular survival and growth dynamics.

Abnormal EPF function is linked with cancer progression and regenerative disorders. Dysregulation of EPF can lead to uncontrolled cell proliferation and has been observed in certain cancers such as colorectal cancer. Furthermore impaired EPF signaling might affect wound healing processes leading to chronic wounds. During these disease processes EPF interacts with other important signaling molecules such as p53 in cancer to influence disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed : 11422376, PubMed : 1346131, PubMed : 7912672). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
See full target information HSPE1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB223401

Alexa Fluor® 647 Anti-EPF antibody [EPR4476]

primary-antibodies

alexa-fluor-647-epf-antibody-epr4476-ab223401

0

(0 reviews)

Alternative Version
Primary Antibodies

AB223400

Alexa Fluor® 488 Anti-EPF antibody [EPR4476]

primary-antibodies

alexa-fluor-488-epf-antibody-epr4476-ab223400

0

(0 reviews)

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