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AB122186

Anti-EPG5 antibody

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(1 Publication)

Rabbit Polyclonal EPG5 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EPG5 aa 800-950.

View Alternative Names

KIAA1632, EPG5, Ectopic P granules protein 5 homolog

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-EPG5 antibody (AB122186)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPG5 antibody (AB122186)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122186 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EPG5 aa 800-950. The exact immunogen used to generate this antibody is proprietary information.

Q9HCE0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPG5 also known as ectopic P-granules autophagy protein 5 homolog is an important protein involved in autophagy. The protein has a mass of approximately 2572 amino acids. Expression of EPG5 occurs in various tissues with notable levels in the central nervous system. The protein participates in the regulation of autophagosome maturation which is essential for cellular homeostasis and the degradation of damaged organelles and proteins.
Biological function summary

EPG5 plays a significant role in the formation and maturation of autophagosomes acting as a tethering factor between autophagosomes and lysosomes. EPG5 does not function alone; it is part of a larger complex contributing specifically to the late stages of autophagy. This interaction helps ensure the proper fusion of autophagosomes with lysosomes enabling the degradation and recycling of cellular components.

Pathways

EPG5 is a critical component in the autophagy pathway. It interacts directly with RAB7 a small GTPase that is important for vesicle trafficking during autophagosome maturation. This interaction facilitates the fusion of autophagosomes with lysosomes. EPG5 also has ties with the endocytosis pathway highlighting its role in maintaining cellular homeostasis through these interconnected systems.

EPG5 mutations associate with Vici syndrome a rare genetic disorder characterized by multisystem symptoms including agenesis of the corpus callosum and immune deficiency. EPG5 is linked to neurodegenerative diseases as its malfunction can lead to impaired neuronal autophagy and accumulation of protein aggregates. In Vici syndrome the dysfunction of EPG5 disrupts normal autophagic processes and lysosomal activity possibly due to its interactions with other proteins such as RAB7 compounding the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in autophagy. May play a role in a late step of autophagy, such as clearance of autophagosomal cargo. Plays a key role in innate and adaptive immune response triggered by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides from pathogens, and mediated by the nucleotide-sensing receptor TLR9. It is necessary for the translocation of CpG dinucleotides from early endosomes to late endosomes and lysosomes, where TLR9 is located (PubMed : 29130391).
See full target information EPG5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 3:5 PubMed31925335

2020

Nanoarchitecture and dynamics of the mouse enteric glycocalyx examined by freeze-etching electron tomography and intravital microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Willy W Sun,Evan S Krystofiak,Alejandra Leo-Macias,Runjia Cui,Antonio Sesso,Roberto Weigert,Seham Ebrahim,Bechara Kachar
View all publications
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