AB124881

Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684]

Name: Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) | Abcam
Brand: Abcam Limited
SKU: AB124881
Price: 380 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(1 Publication)

Rabbit Recombinant Monoclonal Eph receptor A3 phospho Y779 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

ETK, ETK1, HEK, TYRO4, EPHA3, Ephrin type-A receptor 3, EPH-like kinase 4, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, EK4, hEK4, Human embryo kinase, Eph-like tyrosine kinase 1

3 Images

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Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (AB124881)

All lanes:

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/1000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information. at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

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Lab

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (AB124881)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/10000 dilution

Lane 1:

Untreated HEK293 cell lysate at 10 µg

Lane 2:

HEK293 cell lysate treated with pervanadate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 110 kDa

Observed band size: 133 kDa

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OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (AB124881)

OI-RD Scanning

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OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (AB124881)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5684

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4 phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified, use 1/1000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified, use 1/1000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified, use 1/1000 - 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPH receptors A3 A4 and A5 which are also known as EPHA3 EPHA4 and EPHA5 are part of the receptor tyrosine kinase family. These proteins have a mass around 100-130 kDa. They predominantly express in neural tissues but one can also find them in certain epithelial and mesenchymal tissues. Functionally they play a role in cell-cell communication by binding with ephrin ligands which leads to kinase activation and downstream signaling cascades.

Biological function summary

EPH receptors A3 A4 and A5 are essential for mediating a range of cellular processes including cell migration axon guidance and tissue boundary formation. They do not act in isolation but often form complexes with ephrin ligands such as ephrin-A1 and ephrin-A5. These interactions are critical in developmental processes and maintaining cellular architecture. By influencing cell positioning and movement they significantly contribute to nervous system development and vascular patterning.

Pathways

EPH A3 A4 and A5 integrate into the ephrin signaling pathway and the integrin-mediated signaling pathway. In the ephrin signaling pathway they work in tandem with related proteins such as EPHA2 and EPHB receptors to modulate cellular dynamics. These pathways dictate cytoskeletal organization influencing cellular adhesion and motility impacting a variety of physiological processes including angiogenesis and nerve development.

Altered functions of EPH A3 A4 and A5 associate with cancer and neurodegenerative diseases. In cancer especially glioblastoma and melanoma their overexpression or aberrant activity can lead to enhanced cell proliferation and metastasis partially through interactions with proteins like EPHA2. In neurodegenerative conditions such as Alzheimer's disease dysregulated EPH receptor signaling may contribute to neuronal death and cognitive impairment connected with proteins involved in tau pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

See full target information EPHA3 phospho Y779

Additional targets

EPHA5 phospho Y833,EPHA4 phospho Y779

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 38:8345-8363 PubMed30082414

2018

Protein Tyrosine Phosphatase Receptor Type J (PTPRJ) Regulates Retinal Axonal Projections by Inhibiting Eph and Abl Kinases in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yu,Takafumi Shintani,Yasushi Takeuchi,Takuji Shirasawa,Masaharu Noda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684]

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (AB124881)

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (AB124881)

OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (AB124881)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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Secondary Antibodies

Primary Antibodies

Product promise