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AB188171

Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Eph receptor A5/BSK phospho Y779 + Y833 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

BSK, EHK1, HEK7, TYRO4, EPHA5, Ephrin type-A receptor 5, Brain-specific kinase, EPH homology kinase 1, EPH-like kinase 7, EHK-1, EK7, hEK7

3 Images
Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (AB188171)
  • WB

Unknown

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (AB188171)

This data was developed using ab124881, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (<a href='/en-us/products/primary-antibodies/eph-a3a4a5-phospho-y779-y779-y833-antibody-epr5684-ab124881'>ab124881</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information. at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

false

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (AB188171)
  • WB

Lab

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (AB188171)

This data was developed using ab124881, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (<a href='/en-us/products/primary-antibodies/eph-a3a4a5-phospho-y779-y779-y833-antibody-epr5684-ab124881'>ab124881</a>) at 1/10000 dilution

Lane 1:

Untreated HEK293 cell lysate at 10 µg

Lane 2:

HEK293 cell lysate treated with pervanadate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 110 kDa

Observed band size: 133 kDa

false

OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (AB188171)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (AB188171)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5684

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4 phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab188171 is the carrier-free version of ab124881.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPH receptors A3 A4 and A5 which are also known as EPHA3 EPHA4 and EPHA5 are part of the receptor tyrosine kinase family. These proteins have a mass around 100-130 kDa. They predominantly express in neural tissues but one can also find them in certain epithelial and mesenchymal tissues. Functionally they play a role in cell-cell communication by binding with ephrin ligands which leads to kinase activation and downstream signaling cascades.
Biological function summary

EPH receptors A3 A4 and A5 are essential for mediating a range of cellular processes including cell migration axon guidance and tissue boundary formation. They do not act in isolation but often form complexes with ephrin ligands such as ephrin-A1 and ephrin-A5. These interactions are critical in developmental processes and maintaining cellular architecture. By influencing cell positioning and movement they significantly contribute to nervous system development and vascular patterning.

Pathways

EPH A3 A4 and A5 integrate into the ephrin signaling pathway and the integrin-mediated signaling pathway. In the ephrin signaling pathway they work in tandem with related proteins such as EPHA2 and EPHB receptors to modulate cellular dynamics. These pathways dictate cytoskeletal organization influencing cellular adhesion and motility impacting a variety of physiological processes including angiogenesis and nerve development.

Altered functions of EPH A3 A4 and A5 associate with cancer and neurodegenerative diseases. In cancer especially glioblastoma and melanoma their overexpression or aberrant activity can lead to enhanced cell proliferation and metastasis partially through interactions with proteins like EPHA2. In neurodegenerative conditions such as Alzheimer's disease dysregulated EPH receptor signaling may contribute to neuronal death and cognitive impairment connected with proteins involved in tau pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).
See full target information EPHA5 phospho Y779 + Y833
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 17:3509-3517 PubMed30988731

2019

Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-β-induced PI3K/ATK-activated protein C system.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefeng Shan,Zhiqiang Liu,Muhuyati Wulasihan,Songfeng Ma
View all publications
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