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Rabbit Recombinant Monoclonal Eph receptor A2 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 3 publications.

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Images

Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] (AB185156), expandable thumbnail
  • Western blot - Anti-Eph receptor A2 antibody [EPR17660-120] (AB185156), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor A2 antibody [EPR17660-120] (AB185156), expandable thumbnail
  • Immunoprecipitation - Anti-Eph receptor A2 antibody [EPR17660-120] (AB185156), expandable thumbnail
  • Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] (AB185156), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Mouse
Tested
Tested
Tested
Tested
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells (PubMed:29749928). Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Alternative names

Eck, Myk2, Sek2, Epha2, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2

Recommended products

Rabbit Recombinant Monoclonal Eph receptor A2 antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17660-120
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Eph receptor A2 also known as EphA2 or A2 receptor is a type of protein that functions as a receptor tyrosine kinase. It has an approximate mass of 130 kDa. Researchers find this receptor widely expressed in various tissues including epithelial cells and some neuronal populations. EphA2 is involved in cell positioning movement and organization by interacting with ephrin ligands notably ephrin A1. The receptor-ligand interaction triggers bidirectional signaling that affects cell structure and function.

Biological function summary

Eph receptor A2 participates in important cellular processes such as angiogenesis cell proliferation and migration. It often functions as part of larger signaling networks or complexes that include other Eph receptors and ephrin ligands. These interactions help regulate cell adhesion and detachment which are important for embryonic development and tissue organization. EphA2 studies often use fluorescent labeling strategies such as Alexa 588 to track its expression and activity in cells.

Pathways

Eph receptor A2 integrates into the PI3K/AKT and MAPK signaling pathways which are important for cell survival growth and differentiation. It interacts with proteins like Ras and ERK within these pathways to modulate their functions. EphA2 signaling influences cytoskeletal dynamics and morphogenesis making it an important component in maintaining tissue architecture. Research also indicates a connection between EphA2 and the APC protein highlighting its role in the Wnt signaling pathway.

Associated diseases and disorders

The Eph receptor A2 shows significant involvement in cancer and inflammatory conditions. Overexpression or dysregulation of EphA2 occurs frequently in various cancers including breast and prostate cancer correlating with increased tumor aggressiveness and metastasis. The receptor also interacts with other proteins such as APC which links to tumorigenesis and cancer progression. In immunology EphA2 plays a part in chronic inflammatory diseases by modulating immune cell migration and vascular inflammation. Understanding these connections provides insights for potential therapeutic targets using molecular interventions like A2 antibodies and APC-based strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156), expandable thumbnail

    Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156)

    Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast cell line) cell line labeling Eph receptor A2 with ab185156 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Total viable cells were gated for the FC image.

  • Western blot - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156), expandable thumbnail

    Western blot - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156)

    Eph receptor A2 Western blot staining using rabbit Anti-Eph receptor A2 antibody

    Exposure times Lane 1-3: 3 seconds; Lane 4: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

    Lane 2: L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 20 µg

    Lane 3: F9 (mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg

    Lane 4: C6 (rat glial tumor cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 108 kDa

    Observed band size: 130 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156)

    Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Eph receptor A2 with ab185156 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on NIH/3T3 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156), expandable thumbnail

    Immunoprecipitation - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156)

    Eph receptor A2 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryonic fibroblast cell line) lysate with ab185156 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab185156 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (Input).

    Lane 2: ab185156 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab185156 in NIH/3T3 whole cell lysate.

    Exposure time: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156)

    Predicted band size: 108 kDa

  • Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156), expandable thumbnail

    Flow Cytometry - Anti-Eph receptor A2 antibody [EPR17660-120] (ab185156)

    Flow cytometry overlay histogram showing left NIH3T3 positive cells and right negative B16-F10 stained with ab185156 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interactionfollowed by the antibody (ab185156) (1x 106 in 100μl at 5.0 μg/ml (1/424)) for 30min on ice.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

    Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

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Product protocols

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