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Rabbit Recombinant Monoclonal Eph receptor A2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-Eph receptor A2 antibody [EPR18735-112] - BSA and Azide free (Capture) (AB242613), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.Acts as a receptor for human cytomegalovirus (HCMV) to mediate viral entry and fusion in glioblastoma cells.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Eph receptor A2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR18735-112

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab242613 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human EphA2. The recommended pair for sandwich ELISA is:
Capture: ab242613, Human EphA2 Capture Antibody (unconjugated)
Detector: Anti-Eph receptor A2 antibody [EPR18735-118] - BSA and Azide free (Detector) ab242859, Human EphA2 Detector Antibody (unconjugated)
The reference range value is 7.81 - 500 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Eph receptor A2 also known as EphA2 or A2 receptor is a type of protein that functions as a receptor tyrosine kinase. It has an approximate mass of 130 kDa. Researchers find this receptor widely expressed in various tissues including epithelial cells and some neuronal populations. EphA2 is involved in cell positioning movement and organization by interacting with ephrin ligands notably ephrin A1. The receptor-ligand interaction triggers bidirectional signaling that affects cell structure and function.

Biological function summary

Eph receptor A2 participates in important cellular processes such as angiogenesis cell proliferation and migration. It often functions as part of larger signaling networks or complexes that include other Eph receptors and ephrin ligands. These interactions help regulate cell adhesion and detachment which are important for embryonic development and tissue organization. EphA2 studies often use fluorescent labeling strategies such as Alexa 588 to track its expression and activity in cells.

Pathways

Eph receptor A2 integrates into the PI3K/AKT and MAPK signaling pathways which are important for cell survival growth and differentiation. It interacts with proteins like Ras and ERK within these pathways to modulate their functions. EphA2 signaling influences cytoskeletal dynamics and morphogenesis making it an important component in maintaining tissue architecture. Research also indicates a connection between EphA2 and the APC protein highlighting its role in the Wnt signaling pathway.

Associated diseases and disorders

The Eph receptor A2 shows significant involvement in cancer and inflammatory conditions. Overexpression or dysregulation of EphA2 occurs frequently in various cancers including breast and prostate cancer correlating with increased tumor aggressiveness and metastasis. The receptor also interacts with other proteins such as APC which links to tumorigenesis and cancer progression. In immunology EphA2 plays a part in chronic inflammatory diseases by modulating immune cell migration and vascular inflammation. Understanding these connections provides insights for potential therapeutic targets using molecular interventions like A2 antibodies and APC-based strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Sandwich ELISA - Anti-Eph receptor A2 antibody [EPR18735-112] - BSA and Azide free (Capture) (ab242613), expandable thumbnail

    Sandwich ELISA - Anti-Eph receptor A2 antibody [EPR18735-112] - BSA and Azide free (Capture) (ab242613)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Human EphA2 ELISA Kit ab219051).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com