Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free

• WB

Lab

Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (AB290755)

This data was developed using ab273118, the same antibody clone in a different buffer formulation.

Blocking / Diluent buffer and concentration : Intercept^® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

Lanes 1-4 : Merged signal (red and green). Green - ab273118 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.

ab273118 Anti-Ephrin type-A receptor 2 antibody [EPR24711-11] was shown to specifically react with Ephrin type-A receptor 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line (ab264795) was used. Wild-type and Ephrin type-A receptor 2 knockout samples were subjected to SDS-PAGE. ab273118 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (<a href='/en-us/products/primary-antibodies/eph-receptor-a2-antibody-epr24711-11-ab273118'>ab273118</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate. at 20 µg

Lane 2:

Ephrin type-A receptor 2 knockout HeLa (<a href='/en-us/products/cell-lines/human-epha2-eph-receptor-a2-knockout-hela-cell-line-ab264795'>ab264795</a>) whole cell lysate. at 20 µg

Lane 3:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate. at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate. at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 108 kDa

Observed band size: 130 kDa

false

Exposure time: 81s

• WB

Lab

Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (AB290755)

This data was developed using ab273118, the same antibody clone in a different buffer formulation.

Blocking / Diluent buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] (<a href='/en-us/products/primary-antibodies/eph-receptor-a2-antibody-epr24711-11-ab273118'>ab273118</a>) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 108 kDa

Observed band size: 130 kDa

false

Exposure time: 81s