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Rat Monoclonal Eph receptor A2 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Epha2.


Images

Key facts

Isotype

IgG1

Host species

Rat

Storage buffer

Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Mouse Epha2. The exact immunogen used to generate this antibody is proprietary information. Database link Q03145
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-P
Human
Tested
Expected
Mouse
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1 µg for 106 Cells

Notes

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/200

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

Alternative names

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Rat Monoclonal Eph receptor A2 antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Epha2.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Mouse Epha2. The exact immunogen used to generate this antibody is proprietary information. Database link Q03145
Carrier free

Yes

Clone number

RM-0051-8F21

Purification technique

Affinity purification Immunogen

Specificity

ab73254 is specific for Eph receptor A2. It does not cross react with recombinant mouse Eph receptors A3, 4, 6, 7, 8, and Eph receptors B1, 2, 3, 4, or 6.

Concentration
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Purification notes

ab73254 was produced from a hybridoma (mouse myeloma fused withspleen cells from a rat immunized with recombinant mouse EphA2 extracellular domain.The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 µm filtered solution.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Eph receptor A2 participates in important cellular processes such as angiogenesis cell proliferation and migration. It often functions as part of larger signaling networks or complexes that include other Eph receptors and ephrin ligands. These interactions help regulate cell adhesion and detachment which are important for embryonic development and tissue organization. EphA2 studies often use fluorescent labeling strategies such as Alexa 588 to track its expression and activity in cells.

Activity summary

Eph receptor A2 also known as EphA2 or A2 receptor is a type of protein that functions as a receptor tyrosine kinase. It has an approximate mass of 130 kDa. Researchers find this receptor widely expressed in various tissues including epithelial cells and some neuronal populations. EphA2 is involved in cell positioning movement and organization by interacting with ephrin ligands notably ephrin A1. The receptor-ligand interaction triggers bidirectional signaling that affects cell structure and function.

Pathways

Eph receptor A2 integrates into the PI3K/AKT and MAPK signaling pathways which are important for cell survival growth and differentiation. It interacts with proteins like Ras and ERK within these pathways to modulate their functions. EphA2 signaling influences cytoskeletal dynamics and morphogenesis making it an important component in maintaining tissue architecture. Research also indicates a connection between EphA2 and the APC protein highlighting its role in the Wnt signaling pathway.

Associated diseases and disorders

The Eph receptor A2 shows significant involvement in cancer and inflammatory conditions. Overexpression or dysregulation of EphA2 occurs frequently in various cancers including breast and prostate cancer correlating with increased tumor aggressiveness and metastasis. The receptor also interacts with other proteins such as APC which links to tumorigenesis and cancer progression. In immunology EphA2 plays a part in chronic inflammatory diseases by modulating immune cell migration and vascular inflammation. Understanding these connections provides insights for potential therapeutic targets using molecular interventions like A2 antibodies and APC-based strategies.

Product promise

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Full details and terms and conditions can be found here:
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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)

    ab73254 at 1/200 dilution, staining Eph receptor A2 in mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • Flow Cytometry - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254), expandable thumbnail

    Flow Cytometry - Anti-Eph receptor A2 antibody [RM-0051-8F21] (ab73254)

    Overlay histogram showing A431 cells stained with ab73254 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73254, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was goat anti-rat DyLigt 488 (IgG H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG1 [RTK2071] (ab18412, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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