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AB196899

Anti-Eph receptor A2+A3+A4 antibody

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(1 Publication)

Rabbit Polyclonal Eph receptor A2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EPHA3.

View Alternative Names

ECK, EPHA2, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ETK, ETK1, HEK, TYRO4, EPHA3, Ephrin type-A receptor 3, EPH-like kinase 4, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, EK4, hEK4, Human embryo kinase, Eph-like tyrosine kinase 1, HEK8, SEK, TYRO1, EPHA4, Ephrin type-A receptor 4, EPH-like kinase 8, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK, EK8, hEK8

2 Images
Western blot - Anti-Eph receptor A2+A3+A4 antibody (AB196899)
  • WB

Supplier Data

Western blot - Anti-Eph receptor A2+A3+A4 antibody (AB196899)

All lanes:

Western blot - Anti-Eph receptor A2+A3+A4 antibody (ab196899) at 1/500 dilution

Lane 1:

A549 cell extract with synthesized peptide

Lane 2:

A549 cell extract

Lane 3:

HepG2 cell extract

Predicted band size: 108-110 kDa

false

Western blot - Anti-Eph receptor A2+A3+A4 antibody (AB196899)
  • WB

Supplier Data

Western blot - Anti-Eph receptor A2+A3+A4 antibody (AB196899)

All lanes:

Western blot - Anti-Eph receptor A2+A3+A4 antibody (ab196899) at 1/500 dilution

Lane 1:

MCF7 cell extract

Lane 2:

MCF7 cell extract with synthesized peptide

Predicted band size: 108-110 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human EPHA3. The exact immunogen used to generate this antibody is proprietary information.

P29320

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Alternative Products

Primary Antibodies

AB62256

Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

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KO Validated

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Primary Antibodies

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Anti-S100 alpha antibody [EPR5251]

RabMAb|Recombinant

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Primary Antibodies

AB129103

Anti-Eph receptor B1/NET antibody [EPR6457]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)

  • 4
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor A2 A3 and A4 collectively known as the Ephrin type-A receptor group are part of the larger Eph receptor family which is critical in cellular communication mechanisms. These receptors are transmembrane proteins with a molecular weight of approximately 110 to 130 kDa and they are expressed widely throughout tissues such as the brain heart and lungs. These receptors are involved in bidirectional signaling; they interact with ephrin-A ligands to mediate cell-cell contact through forward and reverse signaling pathways.
Biological function summary

These Eph receptors play an essential role in the regulation of cell movement and organization particularly in the nervous system. This receptor group works by forming a receptor-ligand complex with ephrin-A proteins which is important for guiding cell positioning and tissue patterning during development. These interactions regulate axon guidance angiogenesis and the formation of neural networks influencing how cells sort and migrate.

Pathways

Eph receptor A2 A3 and A4 function as key players in the Rho GTPase signaling and MAPK/ERK pathways. These pathways regulate several processes such as cell proliferation differentiation and migration. The Eph receptor family interacts with other proteins like RhoA and Erk1/2 which are critical for modulating cytoskeletal dynamics and activating downstream effects within these pathways impacting cellular structure and signaling.

These Eph receptors are associated with various conditions including cancer and neurodevelopmental disorders. Abnormal expression or mutations in EphA2 for instance have connections to breast cancer where it communicates with proteins like STAT3 to influence tumor growth and progression. In neurodevelopmental disorders altered signaling involving Eph receptors can impact interactions with key proteins like NRG1 leading to abnormal brain structure formation and behavioral outcomes. Understanding these pathways and interactions provides insight into potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.. Acts as a receptor for human cytomegalovirus (HCMV) to mediate viral entry and fusion in glioblastoma cells.
See full target information EPHA2

Additional targets

EPHA4,EPHA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:6519 PubMed28747680

2017

Glycoengineering of EphA4 Fc leads to a unique, long-acting and broad spectrum, Eph receptor therapeutic antagonist.

Applications

WB

Species

Human

Cassandra L Pegg,Leanne T Cooper,Jing Zhao,Michael Gerometta,Fiona M Smith,Michael Yeh,Perry F Bartlett,Jeffrey J Gorman,Andrew W Boyd
View all publications
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Product promise

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