JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB126261

Anti-Eph receptor A3 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal Eph receptor A3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EPHA3 aa 1-350.

View Alternative Names

ETK, ETK1, HEK, TYRO4, EPHA3, Ephrin type-A receptor 3, EPH-like kinase 4, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, EK4, hEK4, Human embryo kinase, Eph-like tyrosine kinase 1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A3 antibody (AB126261)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A3 antibody (AB126261)

ab126261 at 1/500 dilution staining Eph receptor A3 in paraffin embedded Human gastric carcinoma tissue by Immunohistochemistry.

Western blot - Anti-Eph receptor A3 antibody (AB126261)
  • WB

Supplier Data

Western blot - Anti-Eph receptor A3 antibody (AB126261)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Eph receptor A3 antibody (ab126261) at 1/1500 dilution

Lane 1:

CaCo-2 whole cell extracts at 30 µg

Lane 2:

MCF-7 whole cell extracts at 30 µg

Lane 3:

22RV1 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Eph receptor A3 antibody (AB126261)
  • WB

Supplier Data

Western blot - Anti-Eph receptor A3 antibody (AB126261)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Eph receptor A3 antibody (ab126261) at 1/1500 dilution

All lanes:

Molt-4 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Eph receptor A3 antibody (AB126261)
  • WB

Supplier Data

Western blot - Anti-Eph receptor A3 antibody (AB126261)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Eph receptor A3 antibody (ab126261) at 1/1500 dilution

All lanes:

NCI-H929 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Eph receptor A3 antibody (AB126261)
  • WB

Supplier Data

Western blot - Anti-Eph receptor A3 antibody (AB126261)

Samples were separated by 7.5% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Eph receptor A3 antibody (ab126261) at 1/1500 dilution

All lanes:

NIH-3T3 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-Eph receptor A3 antibody (AB126261)
  • WB

Supplier Data

Western blot - Anti-Eph receptor A3 antibody (AB126261)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Eph receptor A3 antibody (ab126261) at 1/1500 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Mouse muscle tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EPHA3 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

P29320

style
left-column

Alternative Products

Primary Antibodies

AB217799

Anti-Eph receptor A3 antibody

Flow Cytometry - Anti-Eph receptor A3 antibody (AB217799)

  • 0
  • 0
Primary Antibodies

AB191565

Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)]

RabMAb|Recombinant

Western blot - Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] (AB191565)

  • 0
  • 0

Complementary Products

Assay Kits

AB303749

Human Eph receptor A3 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Eph receptor A3 ELISA Kit (AB303749)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB109384

Anti-S100 alpha antibody [EPR5251]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (AB109384)

  • 0
  • 0
Primary Antibodies

AB129103

Anti-Eph receptor B1/NET antibody [EPR6457]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor A3 also known as EphA3 is a member of the ephrin receptor subfamily of protein-tyrosine kinases. This receptor with a mass of approximately 120 kDa plays a role in cell-cell communication mainly by interacting with ephrin-A ligands. EphA3 is expressed in various tissues including the nervous system developing tissues and some adult tissues like the lungs and prostate. Its localization varies with the developmental stage and tissue type reflecting its role in numerous processes.
Biological function summary

EphA3 influences cellular organization movement and positioning through its involvement in contact-dependent signaling. It acts as part of a larger receptor-ligand complex which mediates bidirectional signaling between cells. This interaction generally leads to changes in the cytoskeleton impacting cell shape and locomotion. EphA3’s signaling can regulate developmental processes such as axon guidance angiogenesis and tissue boundary formation.

Pathways

EphA3 modulates various important signaling pathways such as the PI3K/AKT pathway and the Rho family GTPase pathway. It interacts with proteins like Ephrin-A5 and other Eph receptors coordinating complex cellular signaling networks. These interactions contribute to the regulation of cell proliferation survival and migration influencing tissue patterning and organ development.

EphA3 has associations with cancer and neurological disorders. It has ties to various cancers like leukemia where it is implicated in proliferation and migration of malignant cells. In the context of neurological conditions EphA3 plays a role in neurodevelopmental disorders. In these diseases connections with other ephrin receptors and proteins involved in cell signaling become critical as they influence pathogenic pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (PubMed : 11870224, PubMed : 12794130). Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5 (By similarity). Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration (PubMed : 11870224). Also activated by EFNA1, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development (PubMed : 12794130). Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development (By similarity).
See full target information EPHA3
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 14:1378087 PubMed38952552

2024

A pan-cancer analysis of EphA family gene expression and its association with prognosis, tumor microenvironment, and therapeutic targets.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Cui,Chengwang Liu,Xuechao Wang,Yiping Xiang

Cancers 15: PubMed36765579

2023

EPHA3 Could Be a Novel Prognosis Biomarker and Correlates with Immune Infiltrates in Bladder Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junpeng Liu,Zewen Zhou,Yifan Jiang,Yuzhao Lin,Yunzhi Yang,Chongjiang Tian,Jinwen Liu,Hao Lin,Bin Huang

Clinical cancer research : an official journal of the American Association for Cancer Research 29:261-270 PubMed36260525

2022

Gene Expression Profiling Identifies Two Chordoma Subtypes Associated with Distinct Molecular Mechanisms and Clinical Outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Bai,Jianxin Shi,Yazhuo Zhang,Chuzhong Li,Yujia Xiong,Hela Koka,Difei Wang,Tongwu Zhang,Lei Song,Wen Luo,Bin Zhu,Belynda Hicks,Amy Hutchinson,Erin Kirk,Melissa A Troester,Mingxuan Li,Yutao Shen,Tianshun Ma,Junmei Wang,Xing Liu,Shuai Wang,Songbai Gui,Mary L McMaster,Stephen J Chanock,Dilys M Parry,Alisa M Goldstein,Xiaohong R Yang

Antioxidants (Basel, Switzerland) 11: PubMed35624662

2022

Indolium 1 Exerts Activity against Vemurafenib-Resistant Melanoma In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Rakan Radi,Christina Huang,Justin Elsey,Yoon H Jung,Victor G Corces,Jack L Arbiser
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com