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AB217799

Anti-Eph receptor A3 antibody

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(1 Publication)

Rabbit Polyclonal Eph receptor A3 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EPHA3 aa 400-500 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ETK, ETK1, HEK, TYRO4, EPHA3, Ephrin type-A receptor 3, EPH-like kinase 4, Tyrosine-protein kinase TYRO4, Tyrosine-protein kinase receptor ETK1, EK4, hEK4, Human embryo kinase, Eph-like tyrosine kinase 1

1 Images
Flow Cytometry - Anti-Eph receptor A3 antibody (AB217799)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Eph receptor A3 antibody (AB217799)

Flow cytometric analysis of Human 293T cells labeling Eph receptor A3 with ab217799 at 1/20 dilution for 30 minutes followed by incubation with a FITC conjugated secondary (green) compared to unstained cells (blue), secondary only (light blue) and isotype control (orange).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human EPHA3 aa 400-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P29320

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor A3 also known as EphA3 is a member of the ephrin receptor subfamily of protein-tyrosine kinases. This receptor with a mass of approximately 120 kDa plays a role in cell-cell communication mainly by interacting with ephrin-A ligands. EphA3 is expressed in various tissues including the nervous system developing tissues and some adult tissues like the lungs and prostate. Its localization varies with the developmental stage and tissue type reflecting its role in numerous processes.
Biological function summary

EphA3 influences cellular organization movement and positioning through its involvement in contact-dependent signaling. It acts as part of a larger receptor-ligand complex which mediates bidirectional signaling between cells. This interaction generally leads to changes in the cytoskeleton impacting cell shape and locomotion. EphA3’s signaling can regulate developmental processes such as axon guidance angiogenesis and tissue boundary formation.

Pathways

EphA3 modulates various important signaling pathways such as the PI3K/AKT pathway and the Rho family GTPase pathway. It interacts with proteins like Ephrin-A5 and other Eph receptors coordinating complex cellular signaling networks. These interactions contribute to the regulation of cell proliferation survival and migration influencing tissue patterning and organ development.

EphA3 has associations with cancer and neurological disorders. It has ties to various cancers like leukemia where it is implicated in proliferation and migration of malignant cells. In the context of neurological conditions EphA3 plays a role in neurodevelopmental disorders. In these diseases connections with other ephrin receptors and proteins involved in cell signaling become critical as they influence pathogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
See full target information EPHA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadp8266 PubMed39356756

2024

Activation of NOD1 on tumor-associated macrophages augments CD8 T cell-mediated antitumor immunity in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhang,Qiuyu Jiang,Jialiang Cai,Fansheng Meng,Wenqing Tang,Zhiyong Liu,Xiahui Lin,Wenfeng Liu,Yi Zhou,Xizhong Shen,Ruyi Xue,Ling Dong,Si Zhang
View all publications
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