AB204113

Anti-Eph receptor A7/EPHA7 antibody

Name: Anti-Eph receptor A7/EPHA7 antibody (ab204113) | Abcam
Brand: Abcam Limited
SKU: AB204113

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(1 Publication)

Rabbit Polyclonal Eph receptor A7/EPHA7 antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EPHA7 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

EHK3, HEK11, EPHA7, Ephrin type-A receptor 7, EPH homology kinase 3, EPH-like kinase 11, EHK-3, EK11, hEK11

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A7/EPHA7 antibody (AB204113)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A7/EPHA7 antibody (AB204113)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse embryo tissue labeling Eph receptor A7/EPHA7 with ab204113 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A7/EPHA7 antibody (AB204113)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling Eph receptor A7/EPHA7 with ab204113 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A7/EPHA7 antibody (AB204113)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat testis tissue labeling Eph receptor A7/EPHA7 with ab204113

at 1/200 dilution overnight at 4°C followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human EPHA7 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q15375

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Eph receptor A7 (EPHA7) also known by its synonym EHK3 is part of the Eph family of receptor tyrosine kinases. It has a molecular mass of approximately 130 kDa. EPHA7 is mainly expressed in the brain but also shows presence in other tissues such as lymphoid tissues. This receptor plays a central role in the repulsion signals guiding cell migration and positioning through its interaction with ephrin ligands.

Biological function summary

EPHA7 functions in the organization of the nervous system and immune response regulation. It operates in the context of a receptor-ligand complex that involves ephrin-A ligands which facilitates cellular communication and positioning. By participating in these processes EPHA7 helps shape neural network formation and modulates immune cell interactions. These biological roles highlight its importance in maintaining tissue architecture.

Pathways

EPHA7 participates in key signaling pathways such as the ephrin receptor signaling pathway and the MAPK signaling cascade. This receptor interacts with proteins from these pathways including ephrin-A5 and MAPK influencing processes like cell adhesion and migration. These pathways establish EPHA7 as an important player in developmental processes and contribute to its role in tissue maintenance and repair.

EPHA7 shows connections with cancer and neurodevelopmental disorders. In cancer alterations in EPHA7 expression and function have been linked to tumor progression and metastasis often in conjunction with proteins such as ephrin-A5. Neurodevelopmental disorders also relate to EPHA7 as disruptions in its signaling can impact brain development potentially involving interactions with other Eph receptors. These connections highlight the receptor's importance in disease mechanisms making it a valuable target for therapeutic exploration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 and MAPK3 which are phosphorylated upon activation of EPHA7.

See full target information EPHA7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 48:626-637 PubMed38263609

2024

LRP8 promotes tumorigenesis in ovarian cancer through inhibiting p53 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Yang Zhou,Xiling Yi,Xiaocui Nie

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Eph receptor A7/EPHA7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A7/EPHA7 antibody (AB204113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A7/EPHA7 antibody (AB204113)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A7/EPHA7 antibody (AB204113)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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