AB61765

Anti-Eph receptor B1 + Eph receptor B2 antibody

Name: Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765) | Abcam
Brand: Abcam Limited
SKU: AB61765
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Eph receptor B1/NET antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EPHB1.

View Alternative Names

ELK, EPHT2, HEK6, NET, EPHB1, Ephrin type-B receptor 1, EPH tyrosine kinase 2, EPH-like kinase 6, Neuronally-expressed EPH-related tyrosine kinase, Tyrosine-protein kinase receptor EPH-2, EK6, hEK6

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

HuvEc cells stained wth ab61765 at 1/500 - 1/1000, with and without the immunising peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EPHB1/2 Antibody. The picture on the right is blocked with the synthesized peptide.

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Western blot - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

All lanes:

Western blot - Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells.

Lane 2:

Extracts from HepG2 cells and the immunising peptide.

Predicted band size: 110 kDa

Observed band size: 110 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human EPHB1. The exact immunogen used to generate this antibody is proprietary information.

P54762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Eph receptor B1 and Eph receptor B2 are part of the Eph family of receptor tyrosine kinases known as EphB1 and EphB2. These receptors play significant roles in cell positioning and movement by mediating cell-to-cell interactions. EphB1 has a molecular weight of approximately 108 kDa and EphB2 weighs around 110 kDa. They are expressed in various tissues including the brain where they are involved in nervous system development and synaptic plasticity.

Biological function summary

EphB1 and EphB2 are involved in processes such as neuronal development and angiogenesis. They participate in forming signaling complexes that guide cell sorting and boundary formation. These receptors interact with ephrin-B ligands initiating bidirectional signaling that influences cellular repulsion and adhesion. Their activities are essential for maintaining proper tissue architecture and directing axon pathfinding.

Pathways

EphB1 and EphB2 integrate into the Eph-ephrin signaling pathway and distinct Rho GTPase signaling pathways. These receptors activate intracellular signaling cascades that regulate cytoskeletal dynamics. They also interact with proteins like Src family kinases which modulate signal transduction processes necessary for cell migration and axonal guidance. This signaling contributes to key biological functions like tissue development and maintenance.

EphB1 and EphB2 have associations with cancer progression and neurological disorders. Dysregulation in their signaling can lead to aberrant cell growth and contribute to the invasion of cancerous cells. Additionally alterations in EphB1 and EphB2 function have links to conditions such as Alzheimer's disease. These receptors interact with proteins like amyloid precursor protein influencing pathways that control neural health and survival. Understanding these interactions helps in developing potential therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (By similarity).

See full target information EPHB1

Additional targets

EPHB2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 10:10092-10101 PubMed31966900

2017

Prognostic values of EphB1/B2 and p-EphB1/B2 expression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Li,Ying He,Dan Liu,Li Li,Fei Chen,Jing Ran,Lan Yang,Li Zhang

Nature communications 7:13525 PubMed27892457

2016

Amplification of USP13 drives ovarian cancer metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Cecil Han,Lifeng Yang,Hyun Ho Choi,Joelle Baddour,Abhinav Achreja,Yunhua Liu,Yujing Li,Jiada Li,Guohui Wan,Cheng Huang,Guang Ji,Xinna Zhang,Deepak Nagrath,Xiongbin Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Eph receptor B1 + Eph receptor B2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

Western blot - Anti-Eph receptor B1 + Eph receptor B2 antibody (AB61765)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

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