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Rabbit Recombinant Monoclonal Eph receptor B2 antibody. Suitable for WB and reacts with Human, Mouse, African green monkey samples. Cited in 5 publications.


Images

Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103), expandable thumbnail
  • OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103), expandable thumbnail
  • Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IFIHC-P
Human
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Predicted
Not recommended
Not recommended
African green monkey
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, African green monkey, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
African green monkey
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, African green monkey, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, African green monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation (PubMed:30213874).

Additional Targets

EPHB3, EPHB4, EPHB2

Alternative names

DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5, EPHB2, Ephrin type-B receptor 2, Developmentally-regulated Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase 3, EPH-like kinase 5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3, EK5, hEK5

Recommended products

Rabbit Recombinant Monoclonal Eph receptor B2 antibody. Suitable for WB and reacts with Human, Mouse, African green monkey samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6457
Purification technique
Affinity purification Protein A
Specificity

Customer data indicates ab129103 reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence.

Dissociation constant
2.13 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Eph receptor B1 also called NET or B1 receptor is a member of the Eph receptor family. It plays roles in various cellular processes through its mechanical action as a receptor tyrosine kinase. The Eph receptor B1 primarily mediates signaling by binding to ephrin-B ligands. This interaction triggers bidirectional signaling between cells influencing cell positioning shape and movement. Eph receptor B1 which has a molecular weight of approximately 120 kDa expresses highly in tissues like the brain and testes suggesting its importance in these organ systems.

Biological function summary

Eph receptor B1 affects cell adhesion migration and tissue patterning by participating in complex networks. These receptors and their ephrin ligands form a complex that enables cross-communication between adjacent cells. Eph receptor B1 modulates processes like synapse formation in the brain thereby impacting neural development and connectivity. As a result it plays a role in maintaining cellular architecture and function in a specific tissue context.

Pathways

Eph receptor B1 is an integral part of the Eph/Ephrin signaling pathway which influences developmental processes and cellular interactions. In this pathway Eph receptor B1 works alongside proteins like ephrin-B to mediate cell-cell communication and signal transduction. This pathway has implications for the formation and remodeling of blood vessels suggesting further links to the angiogenesis pathway. Such pathways are important for translating external signals into cellular responses shaping the biology of tissues and organs.

Associated diseases and disorders

Alterations in Eph receptor B1 function can relate to conditions such as cancer and neurological disorders. In cancers aberrant Eph receptor B1 signaling can promote tumor growth and metastasis with the receptor often associating with proteins like ephrin-B to drive tumorigenic processes. In the context of neurological diseases like Alzheimer's disease disruptions in Eph receptor B1 signaling can affect neural connectivity and function illustrating its role in maintaining brain health. Understanding these connections helps clarify the receptor's impact on disease development and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103), expandable thumbnail

    Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)

    Eph receptor B1/NET Western blot staining using rabbit Anti-Eph receptor B1/NET antibody

    All lanes: Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution

    Lane 1: MDA-MB-435 cell lysate at 10 µg

    Lane 2: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Lane 4: COS1 cell lysate at 10 µg

    Lane 5: Human fetal brain tissue lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 110 kDa

  • OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103), expandable thumbnail

    OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)

    Eph receptor B1/NET western blot using anti-Eph receptor B1/NET antibody [EPR6457] ab129103. Publication image and figure legend from Wang, L., Peng, Q., et al., 2020, J Cancer, PubMed 32368295.


    ab129103 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab129103 please see the product overview.

    Ligand-dependent EphB1 signaling inhibits cancer cell migration and invasion through inducing the phosphorylation of EphB1. (A) Western blot was conducted to test the phosphorylation of EphB1 after transfection of EphB1 or treatment of EphrinB2-Fc; (B) Transwell assay was conducted to test the effect of EphB1 wt and mutant upon treatment of ligand EphrinB2-Fc on the migration and invasion of NSCLC cells. Number of cells were counted and shown in the column graph on the right of the corresponding pictures. Data are mean ± SD of three independent experiments. ** P < 0.01, *** P < 0.001, ns: no significance.

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