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AB129103

Anti-Eph receptor B1/NET antibody [EPR6457]

4

(3 Reviews)

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(7 Publications)

Rabbit Recombinant Monoclonal Eph receptor B2 antibody. Suitable for WB and reacts with Human, Mouse, African green monkey samples. Cited in 7 publications.

View Alternative Names

DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5, EPHB2, Ephrin type-B receptor 2, Developmentally-regulated Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase 3, EPH-like kinase 5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3, EK5, hEK5

3 Images
Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)
  • WB

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Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)

All lanes:

Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution

Lane 1:

MDA-MB-435 cell lysate at 10 µg

Lane 2:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Lane 4:

COS1 cell lysate at 10 µg

Lane 5:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 110 kDa

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OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)
  • OI-RD Scanning

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OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)
  • WB

CiteAb

Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (AB129103)

Eph receptor B1/NET western blot using anti-Eph receptor B1/NET antibody [EPR6457] ab129103. Publication image and figure legend from Wang, L., Peng, Q., et al., 2020, J Cancer, PubMed 32368295.

ab129103 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab129103 please see the product overview.

Ligand-dependent EphB1 signaling inhibits cancer cell migration and invasion through inducing the phosphorylation of EphB1. (A) Western blot was conducted to test the phosphorylation of EphB1 after transfection of EphB1 or treatment of EphrinB2-Fc; (B) Transwell assay was conducted to test the effect of EphB1 wt and mutant upon treatment of ligand EphrinB2-Fc on the migration and invasion of NSCLC cells. Number of cells were counted and shown in the column graph on the right of the corresponding pictures. Data are mean ± SD of three independent experiments. ** p < 0.01, *** p < 0.001, ns : no significance.

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  • Carrier free

    Anti-Eph receptor B1/NET antibody [EPR6457] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6457

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, African green monkey

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Customer data indicates ab129103 reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor B1 also called NET or B1 receptor is a member of the Eph receptor family. It plays roles in various cellular processes through its mechanical action as a receptor tyrosine kinase. The Eph receptor B1 primarily mediates signaling by binding to ephrin-B ligands. This interaction triggers bidirectional signaling between cells influencing cell positioning shape and movement. Eph receptor B1 which has a molecular weight of approximately 120 kDa expresses highly in tissues like the brain and testes suggesting its importance in these organ systems.
Biological function summary

Eph receptor B1 affects cell adhesion migration and tissue patterning by participating in complex networks. These receptors and their ephrin ligands form a complex that enables cross-communication between adjacent cells. Eph receptor B1 modulates processes like synapse formation in the brain thereby impacting neural development and connectivity. As a result it plays a role in maintaining cellular architecture and function in a specific tissue context.

Pathways

Eph receptor B1 is an integral part of the Eph/Ephrin signaling pathway which influences developmental processes and cellular interactions. In this pathway Eph receptor B1 works alongside proteins like ephrin-B to mediate cell-cell communication and signal transduction. This pathway has implications for the formation and remodeling of blood vessels suggesting further links to the angiogenesis pathway. Such pathways are important for translating external signals into cellular responses shaping the biology of tissues and organs.

Alterations in Eph receptor B1 function can relate to conditions such as cancer and neurological disorders. In cancers aberrant Eph receptor B1 signaling can promote tumor growth and metastasis with the receptor often associating with proteins like ephrin-B to drive tumorigenic processes. In the context of neurological diseases like Alzheimer's disease disruptions in Eph receptor B1 signaling can affect neural connectivity and function illustrating its role in maintaining brain health. Understanding these connections helps clarify the receptor's impact on disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation (PubMed : 30213874).
See full target information EPHB2

Additional targets

EPHB3,EPHB4,EPHB2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:1256-1281 PubMed38429579

2024

PHF6-mediated transcriptional control of NSC via Ephrin receptors is impaired in the intellectual disability syndrome BFLS.

Applications

Unspecified application

Species

Unspecified reactive species

Dilan Rasool,Audrey Burban,Ahmad Sharanek,Ariel Madrigal,Jinghua Hu,Keqin Yan,Dianbo Qu,Anne K Voss,Ruth S Slack,Tim Thomas,Azad Bonni,David J Picketts,Vahab D Soleimani,Hamed S Najafabadi,Arezu Jahani-Asl

BMC cancer 22:1364 PubMed36581816

2022

mA RNA methylation regulator-based signature for prognostic prediction and its potential immunological role in uterine corpus endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Fang,Peipei Wang,Haoyu Huang,Mingquan Ye,Xiaocen Liu,Qingqing Li

Cell death & disease 13:980 PubMed36402751

2022

Cell-cell contact-driven EphB1 cis- and trans- signalings regulate cancer stem cells enrichment after chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lujuan Wang,Qiu Peng,Yaohuan Xie,Na Yin,Jiaqi Xu,Anqi Chen,Junqi Yi,Wenhua Shi,Jingqun Tang,Juanjuan Xiang

Journal of Cancer 11:4123-4131 PubMed32368295

2020

Ligand-independent EphB1 signaling mediates TGF-β-activated CDH2 and promotes lung cancer cell invasion and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Lujuan Wang,Qiu Peng,Buqing Sai,Leliang Zheng,Jiaqi Xu,Na Yin,Xiang Feng,Juanjuan Xiang

Molecular medicine reports 18:2171-2181 PubMed29901151

2018

Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide‑induced intestinal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xiong,Kai-Xue Li,Hong Wei,Lu Jiao,Shao-Yong Yu,Li Zeng

ACS chemical biology 12:1499-1503 PubMed28459525

2017

A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein Kinases.

Applications

Unspecified application

Species

Unspecified reactive species

Alvin Kung,Marianne Schimpl,Arunika Ekanayake,Ying-Chu Chen,Ross Overman,Chao Zhang

eLife 5: PubMed27350048

2016

EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Krusche,Cristina Ottone,Melanie P Clements,Ewan R Johnstone,Katrin Goetsch,Huang Lieven,Silvia G Mota,Poonam Singh,Sanjay Khadayate,Azhaar Ashraf,Timothy Davies,Steven M Pollard,Vincenzo De Paola,Federico Roncaroli,Jorge Martinez-Torrecuadrada,Paul Bertone,Simona Parrinello
View all publications
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