Rabbit Recombinant Monoclonal Eph receptor B3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Images
![Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--western-blot-img6722.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)")
![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--immunocytochemistry-immunofluorescence-img38087.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)")
![Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--western-blot-img113660.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)")
![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--immunocytochemistry-immunofluorescence-img89357.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)")
![OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (AB133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--oi-rd-scanning-img150146.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)")
Publications
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- Nature communications 14:64462023Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaitali Chakraborty,Itzel Nissen,Craig A Vincent,Anna-Carin Hägglund,Andreas Hörnblad,Silvia RemeseiroPubMed 37833281
- ACS chemical biology 12:1499-15032017A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein Kinases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlvin Kung et. al.PubMed 28459525
- eLife 5:2016EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Krusche et. al.PubMed 27350048
- Scientific reports 6:282992016TCF7L1 Modulates Colorectal Cancer Growth by Inhibiting Expression of the Tumor-Suppressor Gene EPHB3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Murphy et. al.PubMed 27333864
- Nature communications 6:66192015Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Lin et. al.PubMed 25851023
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Tested | Expected |
| Rat | Not recommended | Not recommended | Tested | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes For unpurified use at 1/1000 - 1/10000. |
Species Rat | Dilution info 1/1000 | Notes For unpurified use at 1/1000 - 1/10000. |
Species Human | Dilution info 1/1000 | Notes For unpurified use at 1/1000 - 1/10000. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes For unpurified use at 1/100 - 1/250. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
Alternative names
ETK2, HEK2, TYRO6, EPHB3, Ephrin type-B receptor 3, EPH-like tyrosine kinase 2, Embryonic kinase 2, Tyrosine-protein kinase TYRO6, EPH-like kinase 2, EK2, hEK2
Recommended products
Rabbit Recombinant Monoclonal Eph receptor B3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8280
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 5.7 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Eph receptor B3 also known as Ephrin type-B receptor 3 or EphB3 is a member of the Eph receptor family. These receptors are important elements in receptor tyrosine kinases notable for their involvement in numerous cellular processes. EphB3 has a molecular mass of approximately 110 kDa. EphB3 is predominantly expressed in the brain but also found in lungs intestines and spinal cord. Its expression pattern hints at its diverse roles in various organ systems.
- Biological function summary
Eph receptor B3 plays important roles in cellular communication influencing cell adhesion migration and differentiation. It often acts by forming receptor complexes with its ligands the ephrins. These interactions initiate bidirectional signaling which regulates the positioning of cells in developing tissues. EphB3 is important in guiding axon pathfinding in neurons and vascular patterning during development.
- Pathways
Eph receptor B3 signaling influences the Rho family GTPase pathway. This pathway is involved in cytoskeletal dynamics and cellular morphology changes. EphB3 also partakes in the MAPK/ERK pathway affecting cell proliferation and survival. Through the MAPK pathway EphB3 is linked with related proteins like EphA2 and EphB2 which share overlapping functions in cellular development and differentiation.
- Associated diseases and disorders
Eph receptor B3 has connections to cancer progression and neurological disorders. Changes in EphB3 expression levels have associations with colorectal cancer affecting cell invasion and metastasis. Additionally altered EphB3 signaling is reported in Alzheimer's disease where it seems to interact with the Amyloid precursor protein (APP) contributing to neurodegenerative processes. These associations highlight EphB3's importance in understanding and potentially targeting these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--western-blot-img6722.jpg "Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)")
Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
Blocking/Diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
Lane 2: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates at 20 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 4: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Lane 6: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
Lane 7: Rat spinal cord lysates at 20 µg
Lane 8: Rat-1 (Rat embryonic fibroblast) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--immunocytochemistry-immunofluorescence-img38087.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)")
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Eph receptor B3 with Purified ab133742 at 1/50 dilution (5.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--western-blot-img113660.jpg "Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)")
Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
All lanes: Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: MOLT4 cell lysate at 10 µg
Lane 5: NIH3T3 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--immunocytochemistry-immunofluorescence-img89357.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)")
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
Immunocytochemical analysis of HeLa cells labelling Eph receptor B3 with unpurified ab133742 at 1/100.
![OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (ab133742), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b3-antibody-epr8280-ab133742--oi-rd-scanning-img150146.jpg "OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)")
OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (ab133742)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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