AB133742

Anti-Eph receptor B3 antibody [EPR8280]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal Eph receptor B3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

ETK2, HEK2, TYRO6, EPHB3, Ephrin type-B receptor 3, EPH-like tyrosine kinase 2, Embryonic kinase 2, Tyrosine-protein kinase TYRO6, EPH-like kinase 2, EK2, hEK2

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

Immunocytochemical analysis of HeLa cells labelling Eph receptor B3 with unpurified ab133742 at 1/100.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Eph receptor B3 with Purified ab133742 at 1/50 dilution (5.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

All lanes:

Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

MOLT4 cell lysate at 10 µg

Lane 5:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 110 kDa

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Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

Lane 6:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 7:

Rat spinal cord lysates at 20 µg

Lane 8:

Rat-1 (Rat embryonic fibroblast) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

OI-RD Scanning

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OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8280

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "For unpurified use at 1/100 - 1/250." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eph receptor B3 also known as Ephrin type-B receptor 3 or EphB3 is a member of the Eph receptor family. These receptors are important elements in receptor tyrosine kinases notable for their involvement in numerous cellular processes. EphB3 has a molecular mass of approximately 110 kDa. EphB3 is predominantly expressed in the brain but also found in lungs intestines and spinal cord. Its expression pattern hints at its diverse roles in various organ systems.

Biological function summary

Eph receptor B3 plays important roles in cellular communication influencing cell adhesion migration and differentiation. It often acts by forming receptor complexes with its ligands the ephrins. These interactions initiate bidirectional signaling which regulates the positioning of cells in developing tissues. EphB3 is important in guiding axon pathfinding in neurons and vascular patterning during development.

Pathways

Eph receptor B3 signaling influences the Rho family GTPase pathway. This pathway is involved in cytoskeletal dynamics and cellular morphology changes. EphB3 also partakes in the MAPK/ERK pathway affecting cell proliferation and survival. Through the MAPK pathway EphB3 is linked with related proteins like EphA2 and EphB2 which share overlapping functions in cellular development and differentiation.

Eph receptor B3 has connections to cancer progression and neurological disorders. Changes in EphB3 expression levels have associations with colorectal cancer affecting cell invasion and metastasis. Additionally altered EphB3 signaling is reported in Alzheimer's disease where it seems to interact with the Amyloid precursor protein (APP) contributing to neurodegenerative processes. These associations highlight EphB3's importance in understanding and potentially targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

See full target information EPHB3

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 14:1732-1754 PubMed38767413

2024

CHD2 Regulates Neuron-Glioma Interactions in Pediatric Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Zhang,Shoufu Duan,Panagiota E Apostolou,Xiaoping Wu,Jun Watanabe,Matthew Gallitto,Tara Barron,Kathryn R Taylor,Pamelyn J Woo,Xu Hua,Hui Zhou,Hong-Jian Wei,Nicholas McQuillan,Kyung-Don Kang,Gregory K Friedman,Peter D Canoll,Kenneth Chang,Cheng-Chia Wu,Rintaro Hashizume,Christopher R Vakoc,Michelle Monje,Guy M McKhann,Joseph A Gogos,Zhiguo Zhang

Nature communications 14:6446 PubMed37833281

2023

Rewiring of the promoter-enhancer interactome and regulatory landscape in glioblastoma orchestrates gene expression underlying neurogliomal synaptic communication.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitali Chakraborty,Itzel Nissen,Craig A Vincent,Anna-Carin Hägglund,Andreas Hörnblad,Silvia Remeseiro

Clinical & translational immunology 11:e1393 PubMed35582627

2022

YTHDF2 promotes temozolomide resistance in glioblastoma by activation of the Akt and NF-κB signalling pathways via inhibiting EPHB3 and TNFAIP3.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Yan-Lan Wang,Kai Qiu,Yi-Qiang Cao,Feng-Jiang Zhang,Hai-Biao Zhao,Xian-Zhi Liu

ACS chemical biology 12:1499-1503 PubMed28459525

2017

A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein Kinases.

Applications

Unspecified application

Species

Unspecified reactive species

Alvin Kung,Marianne Schimpl,Arunika Ekanayake,Ying-Chu Chen,Ross Overman,Chao Zhang

eLife 5: PubMed27350048

2016

EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Krusche,Cristina Ottone,Melanie P Clements,Ewan R Johnstone,Katrin Goetsch,Huang Lieven,Silvia G Mota,Poonam Singh,Sanjay Khadayate,Azhaar Ashraf,Timothy Davies,Steven M Pollard,Vincenzo De Paola,Federico Roncaroli,Jorge Martinez-Torrecuadrada,Paul Bertone,Simona Parrinello

Scientific reports 6:28299 PubMed27333864

2016

TCF7L1 Modulates Colorectal Cancer Growth by Inhibiting Expression of the Tumor-Suppressor Gene EPHB3.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Murphy,Sujash S Chatterjee,Sidharth Jain,Manpreet Katari,Ramanuj DasGupta

Nature communications 6:6619 PubMed25851023

2015

Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Lin,Taofei Yin,Yi I Wu,Takanari Inoue,Andre Levchenko

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Eph receptor B3 antibody [EPR8280]

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

OI-RD Scanning - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise