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Rabbit Recombinant Monoclonal Eph receptor B3 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (AB248634), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (AB248634), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Tested
Expected
Rat
Not recommended
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance also plays an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Alternative names

ETK2, HEK2, TYRO6, EPHB3, Ephrin type-B receptor 3, EPH-like tyrosine kinase 2, Embryonic kinase 2, Tyrosine-protein kinase TYRO6, EPH-like kinase 2, EK2, hEK2

Recommended products

Rabbit Recombinant Monoclonal Eph receptor B3 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8280
Purification technique
Affinity purification Protein A
Dissociation constant
5.7 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248634 is the carrier-free version of Anti-Eph receptor B3 antibody [EPR8280] ab133742.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Eph receptor B3 also known as Ephrin type-B receptor 3 or EphB3 is a member of the Eph receptor family. These receptors are important elements in receptor tyrosine kinases notable for their involvement in numerous cellular processes. EphB3 has a molecular mass of approximately 110 kDa. EphB3 is predominantly expressed in the brain but also found in lungs intestines and spinal cord. Its expression pattern hints at its diverse roles in various organ systems.

Biological function summary

Eph receptor B3 plays important roles in cellular communication influencing cell adhesion migration and differentiation. It often acts by forming receptor complexes with its ligands the ephrins. These interactions initiate bidirectional signaling which regulates the positioning of cells in developing tissues. EphB3 is important in guiding axon pathfinding in neurons and vascular patterning during development.

Pathways

Eph receptor B3 signaling influences the Rho family GTPase pathway. This pathway is involved in cytoskeletal dynamics and cellular morphology changes. EphB3 also partakes in the MAPK/ERK pathway affecting cell proliferation and survival. Through the MAPK pathway EphB3 is linked with related proteins like EphA2 and EphB2 which share overlapping functions in cellular development and differentiation.

Associated diseases and disorders

Eph receptor B3 has connections to cancer progression and neurological disorders. Changes in EphB3 expression levels have associations with colorectal cancer affecting cell invasion and metastasis. Additionally altered EphB3 signaling is reported in Alzheimer's disease where it seems to interact with the Amyloid precursor protein (APP) contributing to neurodegenerative processes. These associations highlight EphB3's importance in understanding and potentially targeting these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634), expandable thumbnail

    Western blot - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eph receptor B3 antibody [EPR8280] ab133742).

    All lanes: Western blot - Anti-Eph receptor B3 antibody [EPR8280] (Anti-Eph receptor B3 antibody [EPR8280] ab133742) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

    Lane 2: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates at 20 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 4: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 5: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

    Lane 6: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

    Lane 7: Rat spinal cord lysates at 20 µg

    Lane 8: Rat-1 (Rat embryonic fibroblast) whole cell lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa

    Observed band size: 110 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B3 antibody [EPR8280] - BSA and Azide free (ab248634)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Eph receptor B3 with Purified Anti-Eph receptor B3 antibody [EPR8280] ab133742 at 1/50 dilution (5.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Eph receptor B3 antibody [EPR8280] ab133742).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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