Rabbit Recombinant Monoclonal Eph receptor B4/HTK antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--flow-cytometry-img403449.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071)")
![Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--flow-cytometry-img403450.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071)")
![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--immunocytochemistry-immunofluorescence-img403451.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071)")
![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--immunocytochemistry-immunofluorescence-img403452.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (AB305071)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
Alternative names
HTK, MYK1, TYRO11, EPHB4, Ephrin type-B receptor 4, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11
Recommended products
Rabbit Recombinant Monoclonal Eph receptor B4/HTK antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23222-2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Eph receptor B4 also known as EphB4 or HTK is a protein belonging to the Ephrin receptor subfamily of receptor tyrosine kinases. The receptor has a molecular weight of about 120 kDa. EphB4 typically interacts with its binding partner ephrin-B2 facilitating bidirectional signaling. These complexes reside in the cell membrane and are mainly found in vascular endothelial cells and various tissues including the heart lungs and skin. Notably this receptor plays a role in cellular processes like cell migration and adhesion.
- Biological function summary
The Eph receptor B4 is a significant regulator of angiogenesis and vascular development. It forms a critical component of a signaling complex that also includes ephrin-B2. During embryonic development EphB4's interaction with ephrin-B2 guides the proper formation and organization of blood vessels. In adult organisms it remains active in maintaining vascular homeostasis. Additionally this receptor impacts neuronal development and repair through modulating axon guidance and synaptic plasticity.
- Pathways
EphB4 engages in various cellular signaling cascades. It features prominently within pathways governing angiogenesis and cell adhesion. EphB4 acts as a central player in the VEGF signaling pathway modulating endothelial cell behavior. The protein’s pathways frequently intersect with other proteins such as FAK and Src influencing cell motility and morphology. Furthermore in the ephrin receptor pathway it cross-talks with other Eph receptors like EphA4 assisting in diverse biological functions.
- Associated diseases and disorders
Abnormal Eph receptor B4 activity associates with several pathological conditions. Dysregulation often links to cancer due to its role in neovascularization and tumor progression. Overexpression of EphB4 in tumors like breast and colorectal cancer suggests a contribution to malignancy by supporting angiogenesis and tumor growth. In vascular disorders impaired EphB4-ephrin-B2 signaling may lead to defects in blood vessel formation. Additionally alterations in this receptor are observed in neurological diseases such as Alzheimer's where EphB4 interacts with tau protein-modifying vascular and neuronal deficiencies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--flow-cytometry-img403449.jpg "Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)")
Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)
This data was developed using Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 786-O (human kidney epithelial cell, Left) / MCF7 (human breast adenocarcinoma epithelial cell, Right) cells labelling Eph receptor B4/HTK with Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control: 786-O (PMID: 34399807).![Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--flow-cytometry-img403450.jpg "Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)")
Flow Cytometry - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)
This data was developed using Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Wild-type 293T (human embryonic kidney epithelial cell, Right) / Eph receptor B4 knockout 293T (Left) cells labelling Eph receptor B4/HTK with Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Positive staining on 293T cells (ab255449), while no staining on Eph receptor B4 knockout 293T cells (Human EPHB4 (Eph receptor B4/HTK) knockout HEK-293T cell line ab266733).![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--immunocytochemistry-immunofluorescence-img403451.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)")
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)
This data was developed using Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EPHB4 KO HEK293T?Human EPHB4 (Eph receptor B4/HTK) knockout HEK-293T cell line ab266733? cells labelling Eph receptor B4/HTK with Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102 at 1/100 (5.15 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in parental HEK293T cell line, but no staining in EPHB4 KO HEK293T cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b4-htk-antibody-epr23222-2-bsa-and-azide-free-ab305071--immunocytochemistry-immunofluorescence-img403452.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)")
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B4/HTK antibody [EPR23222-2] - BSA and Azide free (ab305071)
This data was developed using Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell? cells labelling Eph receptor B4/HTK with Anti-Eph receptor B4/HTK antibody [EPR23222-2] ab305102 at 1/100 (5.15 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing membranous staining in MCF-7 cell line.Negative control: 786-O (PMID: 34399807).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
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