Rabbit Recombinant Monoclonal Eph receptor B6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Eph receptor B6 antibody [EPR29998-586] - BSA and Azide free (Detector) (AB322190), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b6-antibody-epr29998-586-bsa-and-azide-free-detector-ab322190--sandwich-elisa-img437431.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Eph receptor B6 antibody [EPR29998-586] - BSA and Azide free (Detector) (AB322190)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.
Alternative names
Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6
Recommended products
Rabbit Recombinant Monoclonal Eph receptor B6 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR29998-586
- Purification technique
Affinity purification Protein A
- Specificity
This antibody has 25% cross reaction with human Eph receptor B2 and 27% cross reaction with human Eph receptor B3, but does not cross-react with human Eph receptor B1 and Eph receptor B4.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab322190 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Eph receptor B6. The recommended pair for sandwich ELISA is:
Capture: Anti-Eph receptor B6 antibody [EPR29998-504] - BSA and Azide free (Capture/Detector) ab322189, Human Eph receptor B6 Capture Antibody (unconjugated)
Detector: ab322190, Human Eph receptor B6 Detector Antibody (unconjugated)
The reference range value is 15.63-1000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- Activity summary
Eph receptor B6 also known as EphB6 is a member of the Eph receptor family which includes a group of receptor tyrosine kinases. EphB6 has an approximate molecular mass of 108 kDa. Unlike other members of the Eph receptor family it lacks kinase activity due to mutations in its kinase domain. EphB6 is expressed in various tissues with higher expression noted in the thymus brain and certain immune cells. It primarily functions by binding to ephrin ligands particularly ephrin-B family members allowing it to interact within the cellular environment despite its kinase-inactive state.
- Biological function summary
EphB6 plays an important role in cell adhesion migration and regulation of immune responses. Although it forms part of the larger Eph-ephrin signaling complex EphB6 contributes to cellular communication without exhibiting intrinsic catalytic action. Its interactions often influence cytoskeletal organization and can impact cell shape and movement. More specifically EphB6 signaling is modulated through its ability to form complexes with other kinases such as EphB1 or EphB4 which can provide the kinase activity needed for downstream effects.
- Pathways
EphB6 participates in important signaling networks such as the PI3K/AKT and MAPK pathways. These pathways are important for regulating cell survival and proliferation. In the context of these pathways EphB6 interacts with proteins such as PI3K influencing pathway activation and cellular outcomes. Eph-ephrin complexes can also integrate into the MAPK pathway through other Eph receptors facilitating various cellular responses in development and immune function.
- Associated diseases and disorders
Alterations in EphB6 expression or function have been linked to cancer and autoimmune conditions. In cancers like melanoma modifications in EphB6 expression levels or its pathway interactions can affect tumor progression and metastasis. In autoimmune disorders EphB6 functions might alter immune cell behavior contributing to disease pathology. Notably EphB6's interaction with other Eph receptors such as EphB4 has been noted in some cancers showing that the balance of Eph receptor interactions can impact disease outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Eph receptor B6 antibody [EPR29998-586] - BSA and Azide free (Detector) (ab322190), expandable thumbnail](https://content.abcam.com/products/images/eph-receptor-b6-antibody-epr29998-586-bsa-and-azide-free-detector-ab322190--sandwich-elisa-img437431.jpg "Sandwich ELISA - Anti-Eph receptor B6 antibody [EPR29998-586] - BSA and Azide free (Detector) (ab322190)")
Sandwich ELISA - Anti-Eph receptor B6 antibody [EPR29998-586] - BSA and Azide free (Detector) (ab322190)
Sandwich ELISA of Human Eph receptor B6 Antibody Pair - BSA and Azide free ab322188 with the capture antibody Anti-Eph receptor B6 antibody [EPR29998-504] - BSA and Azide free (Capture/Detector) ab322189 dilution at 2 ug/mL and detector antibody ab322190 dilution at 0.5 ug/mL.
Downloads
Product protocols
- Visit the general protocols
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