AB157593

Anti-Ephexin-1 antibody - C-terminal

Name: Anti-Ephexin-1 antibody - C-terminal (ab157593) | Abcam
Brand: Abcam Limited
SKU: AB157593
Price: 535 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(2 Publications)

Rabbit Polyclonal Ephexin-1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Ephexin-1.

View Alternative Names

Ephexin-1, Eph-interacting exchange protein, Neuronal guanine nucleotide exchange factor, NGEF

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ephexin-1 antibody - C-terminal (AB157593)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ephexin-1 antibody - C-terminal (AB157593)

Immunofluorescent analysis of Mouse C2C12 cells, labeling Ephexin-1 with ab157593 at 1/50 dilution. Right image shows cells treated with pervanadate.

Unknown

Western blot - Anti-Ephexin-1 antibody - C-terminal (AB157593)

All lanes:

Western blot - Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution

Lane 1:

A431 cell lysate

Lane 2:

A431 cell lysate, treated with pervanadate (1mM; 30 mins)

Predicted band size: 82 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Mouse Ephexin-1. The exact immunogen used to generate this antibody is proprietary information.

Q8CHT1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephexin-1 also referred to as NGEF (neuronal guanine nucleotide exchange factor) acts as a guanine nucleotide exchange factor (GEF) that specifically activates Rho GTPases by promoting the exchange of GDP for GTP. Ephexin-1 has an approximate mass of 70 kDa. Its expression is widely observed in various tissues with significant levels in the nervous system where it plays important roles in neural development and function.

Biological function summary

Ephexin-1 plays a role in cytoskeletal reorganization by modulating the activity of Rho GTPases which are critical regulators of actin dynamics. It is part of molecular complexes that help control axon guidance and synapse formation in neurons. Through these functions Ephexin-1 influences cell shape motility and growth.

Pathways

Ephexin-1 interacts with neural pathways such as the ephrin signaling pathway. It acts in conjunction with proteins like Eph receptors which are important for cell-cell communication and guidance during developmental processes. Another pathway includes the Rho/ROCK signaling cascade where Ephexin-1 activity leads to changes in actin cytoskeleton dynamics and influences cellular morphology.

Alterations in Ephexin-1 function have been linked to neurodevelopmental disorders and cancers. Mutations or abnormal expression of Ephexin-1 can affect the balance of signaling pathways and cause disruptions in normal neural function. It also relates to conditions like schizophrenia where Eph receptors are also implicated highlighting the interconnected network between Ephexin-1 and Eph receptors in disease etiologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

See full target information NGEF

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomarker research 10:77 PubMed36329557

2022

Loss of miR-637 promotes cancer cell stemness via WASH/IL-8 pathway and serves as a novel prognostic marker in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxing Guo,Jingyao Lian,Yaqing Liu,Bo Dong,Qianyi He,Qitai Zhao,Hongyan Zhang,Yu Qi,Yi Zhang,Lan Huang

Glia 70:1971-1991 PubMed35762396

2022

EphA4 regulates white matter remyelination after ischemic stroke through Ephexin-1/RhoA/ROCK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cui Liu,Song Han,Jiayin Zheng,Hongyu Wang,Shujuan Li,Junfa Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ephexin-1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Ephexin-1 antibody - C-terminal (AB157593)

Western blot - Anti-Ephexin-1 antibody - C-terminal (AB157593)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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