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Rabbit Recombinant Monoclonal Ephrin A1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.

Alternative names

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Rabbit Recombinant Monoclonal Ephrin A1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR1785(2)

Purification technique

Affinity purification Protein A

Dissociation constant

1.59 x 10-10 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

Ephrin A1 contributes to the regulation of cell movement and adhesion. It often forms part of a receptor-ligand complex with Eph receptors on adjacent cells. This interaction controls the guidance of axonal growth angiogenesis and tissue boundary formation. In the nervous system ephrin A1 influences the segregation of synaptic connections playing a role in developing the proper neural circuitry. Its expression patterns suggest its involvement in vascular processes and other critical physiological functions.

Activity summary

Ephrin A1 also known as EFNA1 is a membrane-bound ligand that belongs to the ephrin family of proteins. It has a mass of approximately 22 kDa. Ephrin A1 engages in the binding and activation of Eph receptors particularly EphA2 which mediates bidirectional signaling between cells. This protein is widely expressed in various tissues including the adult brain lungs and liver. It plays a significant role in cell-cell communication especially during developmental processes.

Pathways

Ephrin A1 is significantly involved in various signaling pathways that mediate cellular attachment and repulsion features. Notably it participates in the Eph/ephrin signaling and the Rho family GTPase signaling pathways. Through its interaction with the EphA2 receptor ephrin A1 regulates cytoskeletal dynamics and influences both integrin-mediated adhesion and cell migration. These pathways are important for maintaining cellular architecture and orchestrating cellular responses to environmental cues.

Associated diseases and disorders

Ephrin A1 has been implicated in several pathological conditions notably breast cancer and glioblastoma. It interacts with the EphA2 receptor which is overexpressed in these cancers contributing to tumor development and progression. It is also associated with cancer-related proteins involved in metastatic signaling. Studies show that disrupted ephrin A1/EphA2 interactions can affect tumor angiogenesis and cellular proliferation highlighting its potential as a target for therapeutic intervention in cancer biology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911), expandable thumbnail

    Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)

    All lanes: Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (AB124911) at 1/1000 dilution

    Lane 1: HUVEC cell lysate, treated with TNF alpha at 10 µg

    Lane 2: HUVEC cell lysate at 10 µg

    Lane 3: MCF7 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 24 kDa

    Observed band size: 24 kDa

  • OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911), expandable thumbnail

    OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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