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AB124911

Anti-Ephrin A1 antibody [EPR1785(2)]

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(7 Publications)

Rabbit Recombinant Monoclonal Ephrin A1 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

EPLG1, LERK1, TNFAIP4, EFNA1, Ephrin-A1, EPH-related receptor tyrosine kinase ligand 1, Immediate early response protein B61, Tumor necrosis factor alpha-induced protein 4, LERK-1, TNF alpha-induced protein 4

2 Images
Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (AB124911)
  • WB

Unknown

Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (AB124911)

All lanes:

Western blot - Anti-Ephrin A1 antibody [EPR1785(2)] (ab124911) at 1/1000 dilution

Lane 1:

HUVEC cell lysate, treated with TNF alpha at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (AB124911)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Ephrin A1 antibody [EPR1785(2)] (AB124911)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1785(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin A1 also known as EFNA1 is a membrane-bound ligand that belongs to the ephrin family of proteins. It has a mass of approximately 22 kDa. Ephrin A1 engages in the binding and activation of Eph receptors particularly EphA2 which mediates bidirectional signaling between cells. This protein is widely expressed in various tissues including the adult brain lungs and liver. It plays a significant role in cell-cell communication especially during developmental processes.
Biological function summary

Ephrin A1 contributes to the regulation of cell movement and adhesion. It often forms part of a receptor-ligand complex with Eph receptors on adjacent cells. This interaction controls the guidance of axonal growth angiogenesis and tissue boundary formation. In the nervous system ephrin A1 influences the segregation of synaptic connections playing a role in developing the proper neural circuitry. Its expression patterns suggest its involvement in vascular processes and other critical physiological functions.

Pathways

Ephrin A1 is significantly involved in various signaling pathways that mediate cellular attachment and repulsion features. Notably it participates in the Eph/ephrin signaling and the Rho family GTPase signaling pathways. Through its interaction with the EphA2 receptor ephrin A1 regulates cytoskeletal dynamics and influences both integrin-mediated adhesion and cell migration. These pathways are important for maintaining cellular architecture and orchestrating cellular responses to environmental cues.

Ephrin A1 has been implicated in several pathological conditions notably breast cancer and glioblastoma. It interacts with the EphA2 receptor which is overexpressed in these cancers contributing to tumor development and progression. It is also associated with cancer-related proteins involved in metastatic signaling. Studies show that disrupted ephrin A1/EphA2 interactions can affect tumor angiogenesis and cellular proliferation highlighting its potential as a target for therapeutic intervention in cancer biology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
See full target information EFNA1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112737 PubMed37393620

2023

Dissecting the spermatogonial stem cell niche using spatial transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Shreya Rajachandran,Xin Zhang,Qiqi Cao,Andre L Caldeira-Brant,Xiangfan Zhang,Youngmin Song,Melanie Evans,Orhan Bukulmez,Edward J Grow,Makoto Nagano,Kyle E Orwig,Haiqi Chen

Discover. Oncology 14:64 PubMed37160815

2023

Targeting EFNA1 suppresses tumor progression via the cMYC-modulated cell cycle and autophagy in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Houxiang Jiang,Shaoxiang Wang,Ying Liu,Chaopan Zheng,Lipeng Chen,Kai Zheng,Zhenyu Xu,Yong Dai,Hongtao Jin,Zhiqiang Cheng,Chang Zou,Li Fu,Kaisheng Liu,Xiaoshi Ma

Journal of cell science 134: PubMed34338780

2021

A three-dimensional spheroid-specific role for Wnt-β-catenin and Eph-ephrin signaling in nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Canhui Yi,Sook Ling Lai,Chi Man Tsang,Margarita Artemenko,Maggie Kei Shuen Tang,Stella W Pang,Kwok Wai Lo,Sai Wah Tsao,Alice Sze Tsai Wong

World journal of surgical oncology 19:242 PubMed34399788

2021

Effects of EFNA1 on cell phenotype and prognosis of esophageal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Zhang,Jinning Zhang,Guanlong Pan,Tianhao Guan,Changhao Zhang,An Hao,Yan Li,Hai Ren

Gynecologic oncology 163:181-190 PubMed34391578

2021

MEK inhibition overcomes resistance to EphA2-targeted therapy in uterine cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yutuan Wu,Jie Huang,Cristina Ivan,Yunjie Sun,Shaolin Ma,Lingegowda S Mangala,Bryan M Fellman,Diana L Urbauer,Nicholas B Jennings,Prahlad Ram,Robert L Coleman,Wei Hu,Anil K Sood

Cell death & disease 11:597 PubMed32732965

2020

The miR-1224-5p/TNS4/EGFR axis inhibits tumour progression in oesophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Zhou Shi,Wen-Jun Wang,Yun-Xia Chen,Ze-Wen Fan,Xiu-Feng Xie,Li-Yan Yang,Chen Chang,Yan Cai,Jia-Jie Hao,Ming-Rong Wang,Jie Bai

Cell death and differentiation 24:421-432 PubMed28009350

2016

HIC1 and miR-23~27~24 clusters form a double-negative feedback loop in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbo Wang,Hongwei Liang,Geyu Zhou,Xiuting Hu,Zhengya Liu,Fangfang Jin,Mengchao Yu,Jianfeng Sang,Yong Zhou,Zheng Fu,Chen-Yu Zhang,Weijie Zhang,Ke Zen,Xi Chen
View all publications
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