AB232711

Anti-Ephrin A4 antibody

Name: Anti-Ephrin A4 antibody (ab232711) | Abcam
Brand: Abcam Limited
SKU: AB232711

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(1 Publication)

Rabbit Polyclonal Ephrin A4 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Efna4.

View Alternative Names

Epl4, Eplg4, Lerk4, Efna4, Ephrin-A4, EPH-related receptor tyrosine kinase ligand 4, LERK-4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A4 antibody (AB232711)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A4 antibody (AB232711)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Ephrin A4 with ab232711 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Ephrin A4 antibody (AB232711)

All lanes:

Western blot - Anti-Ephrin A4 antibody (ab232711) at 3 µg/mL

Lane 1:

Mouse spleen tissue lysate

Lane 2:

Mouse ovary tissue lysate

Lane 3:

Mouse thymus tissue lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 22 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A4 antibody (AB232711)

Formalin-fixed, paraffin-embedded mouse liver tissue stained for Ephrin A4 with ab232711 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Ephrin A4 antibody (AB232711)

All lanes:

Western blot - Anti-Ephrin A4 antibody (ab232711) at 3 µg/mL

All lanes:

Recombinant mouse Ephrin A4 protein

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Efna4.

O08542

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin A4 also known as EFNA4 is part of the ephrin family of proteins. It interacts with Eph receptors and primarily functions as a ligand—a molecule that binds to another to form a complex—within this signaling system. Ephrin A4 is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage and its molecular mass is approximately 21 kDa. The expression of Ephrin A4 can be found in various tissues including the brain lungs and kidneys indicating its diverse roles in bodily functions.

Biological function summary

Ephrin A4 plays significant roles in the development and function of the nervous system. It works within the ephrin/Eph receptor signaling system impacting processes such as cell positioning and tissue boundary formation. Ephrin A4 is not an isolated component; it acts as part of a complex with Eph receptors influencing cell adhesion and shape. These interactions guide the cellular processes required for proper embryonic development.

Pathways

Ephrin A4 is involved in the ephrin signaling pathway and the MAPK/ERK pathway. These pathways regulate cell proliferation differentiation and migration which are essential for developmental processes. Ephrin A4 interacts with EphA receptors influencing these signaling cascades. This connection shows its significant influence on various cellular processes including those relevant in neural patterning.

Ephrin A4 has associations with certain cancers and neurodegenerative illnesses. In cancer particularly breast cancer Ephrin A4 dysregulation links to uncontrolled cell proliferation and migration often in connection with EphA2 receptor overexpression. Furthermore in neurodegenerative conditions like Alzheimer's disease altered ephrin signaling involves the degradation of neural structures implicating interactions with EphB2. These associations underline the importance of Ephrin A4 in maintaining cellular and tissue health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils (By similarity).

See full target information Efna4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proteomics 22:e2100028 PubMed35234362

2022

Effects of ABCG1 knockout on proteomic composition of HDL in mice on a chow diet and a high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxiang Wu,Lianfeng Chen,Ziyan Xie,Chenyu Wang,Jiahao Zhang,Xiaowei Yan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ephrin A4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A4 antibody (AB232711)

Western blot - Anti-Ephrin A4 antibody (AB232711)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin A4 antibody (AB232711)

Western blot - Anti-Ephrin A4 antibody (AB232711)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise