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AB221652

Anti-Ephrin B1 antibody

0

(1 Review)

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(1 Publication)

Rabbit Polyclonal Ephrin B1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EFNB1 aa 150-250.

View Alternative Names

EFL3, EPLG2, LERK2, EFNB1, Ephrin-B1, EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, ELK-L, LERK-2

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B1 antibody (AB221652)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B1 antibody (AB221652)

PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for Ephrin B1 (green) using ab221652 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EFNB1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

P98172

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Complementary Products

Primary Antibodies

AB133742

Anti-Eph receptor B3 antibody [EPR8280]

RabMAb|Recombinant

Western blot - Anti-Eph receptor B3 antibody [EPR8280] (AB133742)

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Primary Antibodies

AB53063

Anti-Ephrin B3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B3 antibody (AB53063)

  • 5
  • 1
Primary Antibodies

AB30682

Anti-SREBP2 antibody

Western blot - Anti-SREBP2 antibody (AB30682)

  • 4
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin-B1 also known as B1 protein or Ephrin-B1 protein is a member of the ephrin family with a mass around 25 kDa. Located on the plasma membrane Ephrin-B1 mainly expresses in the nervous system skeletal system and some epithelial tissues. The protein functions as a ligand for the Eph receptor family which plays a role in cell signaling. Ephrin-B1 interacts with Eph receptors through bi-directional signaling influencing cellular communication and movement.
Biological function summary

Ephrin-B1 is critical for cell positioning and boundary formation. It is part of a transmembrane protein complex influencing cell-to-cell interactions. Through its role in adhesion and repulsion Ephrin-B1 helps coordinate developmental processes such as tissue assembly and organ development. The protein serves essential roles in maintaining cellular architecture ensuring that cells adhere and detach properly during development and wound repair.

Pathways

The ephrin-B1 signaling is important to the Eph receptor pathways particularly the EphB forward and reverse signaling pathways. These pathways are well known for their functions in axon guidance and synaptic plasticity linking Ephrin-B1 with the nervous system development. Related proteins in these pathways include EphB2 and EphB4 which work alongside Ephrin-B1 in regulating the dynamics of these pathways facilitating processes like neurite outgrowth and spine morphogenesis.

Ephrin-B1 is associated with cleft palate and craniofrontonasal syndrome. Mutations or dysregulation in Ephrin-B1 can disrupt normal tissue boundary formation and cell migration leading to such developmental disorders. Abnormal interaction between Ephrin-B1 and related proteins such as EphB2 can affect developmental signaling cascades highlighting the importance of this protein in proper craniofacial and tissue development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development (PubMed : 7973638, PubMed : 8070404). Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells (PubMed : 7973638, PubMed : 8070404). Shows high affinity for the receptor tyrosine kinase EPHB1/ELK (PubMed : 7973638, PubMed : 8070404). Can also bind EPHB2 and EPHB3 (PubMed : 8070404). Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
See full target information EFNB1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BioMed research international 2024:2973407 PubMed38449509

2024

Identifying G6PC3 as a Potential Key Molecule in Hypoxic Glucose Metabolism of Glioblastoma Derived from the Depiction of F-Fluoromisonidazole and F-Fluorodeoxyglucose Positron Emission Tomography.

Applications

Unspecified application

Species

Unspecified reactive species

Michinari Okamoto,Shigeru Yamaguchi,Ryosuke Sawaya,Sumire Echizenya,Yukitomo Ishi,Sadahiro Kaneko,Hiroaki Motegi,Takuya Toyonaga,Kenji Hirata,Miki Fujimura
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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