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AB326694

Anti-Ephrin B2 antibody [31D11]

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Rabbit Recombinant Monoclonal Ephrin B2 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

EPLG5, HTKL, LERK5, EFNB2, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, HTK ligand, LERK-5, HTK-L

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody [31D11] (AB326694)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ephrin B2 antibody [31D11] (AB326694)

ICC/IF staining of Ephrin B2 in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.

Samples were incubated with antibody at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.

The cells were incubated 30 minutes at room temperature with phalloidin tritc .

Nuclei were counterstained with DAPI.

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)
  • WB

Supplier Data

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)

All lanes : Primary antibody at 1/1000 dilution

Lane1 : K562. Proteins at 20ug per lane. Secondary : Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size : 50kDa. Observed band size : 50kDa.

All lanes:

Western blot - Anti-Ephrin B2 antibody [31D11] (ab326694) at 1/1000 dilution

All lanes:

K562 at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)
  • WB

Supplier Data

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)

All lanes : Primary antibody at 1/1000 dilution

Lane1 : HEK293 cell lysate. Lane2 : Mouse spleen lysate. (Proteins at 20ug per lane). Secondary : Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution. Predicted band size : 50kDa. Observed band size : 50kDa.

All lanes:

Western blot - Anti-Ephrin B2 antibody [31D11] (ab326694) at 1/1000 dilution

Lane 1:

HEK293 cell lysate at 20 µg

Lane 2:

Mouse spleen lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)
  • WB

Supplier Data

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)

All lanes : Primary antibody at 1/1000 dilution

Lane1 : HeLa. Lane2 : 293. Lane3 : MCF-7. Lane4 : K562. Lane5 : U-87 MG. (Proteins at 20ug per lane). Secondary : Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution. Predicted band size : 50kDa. Observed band size : 50kDa.

All lanes:

Western blot - Anti-Ephrin B2 antibody [31D11] (ab326694) at 1/1000 dilution

Lane 1:

HeLa at 20 µg

Lane 2:

293 at 20 µg

Lane 3:

MCF-7 at 20 µg

Lane 4:

K562 at 20 µg

Lane 5:

U-87 MG at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)
  • WB

Supplier Data

Western blot - Anti-Ephrin B2 antibody [31D11] (AB326694)

All lanes : Primary antibody at 1/1000 dilution

Lane1 : Mouse kidney. Lane2 : Mouse brain. Lane3 : Rat kidney. Lane4 : Rat brain. (Proteins at 20ug per lane). Secondary : Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution. Predicted band size : 50kDa. Observed band size : 50kDa.

All lanes:

Western blot - Anti-Ephrin B2 antibody [31D11] (ab326694) at 1/1000 dilution

Lane 1:

Mouse kidney at 20 µg

Lane 2:

Mouse brain at 20 µg

Lane 3:

Rat kidney at 20 µg

Lane 4:

Rat brain at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG(HRP) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

31D11

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
See full target information EFNB2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com