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AB193634

Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody

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(1 Publication)

Rabbit Polyclonal Ephrin B1 phospho Y324 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EFNB2 pY311.

View Alternative Names

EFL3, EPLG2, LERK2, EFNB1, Ephrin-B1, EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, ELK-L, LERK-2, EPLG5, HTKL, LERK5, EFNB2, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, HTK ligand, LERK-5, HTK-L, EPLG8, LERK8, EFNB3, Ephrin-B3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)

Immunohistochemical analysis of paraffin-embedded Human brain tissue sections labeling Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) with ab193634 at a 1/50 dilution. Right image is treated with blocking peptide.

Western blot - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)
  • WB

Supplier Data

Western blot - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (AB193634)

All lanes:

Western blot - Anti-Ephrin B2 (phospho Y311) + Ephrin B3 (phospho Y318) + Ephrin B1 (phospho Y324) antibody (ab193634) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells treated with serum

Lane 2:

Extracts from HepG2 cells treated with serum with antigen-specific peptide

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human EFNB1 pY324 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. Database link P98172

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human EFNB3 pY318. Database link Q15768

Specificity

ab193634 detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324/311/318 respectivly.

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Primary Antibodies

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Primary Antibodies

AB289367

Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [HL173] - BSA and Azide free (AB289367)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab193634 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin B1 Ephrin B2 and Ephrin B3 also known as EFNB1 EFNB2 and EFNB3 are transmembrane proteins involved in cell communication. These proteins belong to the ephrin family and typically exhibit molecular masses ranging from 25 to 30 kDa. They are predominantly expressed in the nervous system vasculature and various tissues such as bones and liver. By binding to Eph receptors which are a subclass of receptor tyrosine kinases ephrins play a major role in bidirectional signaling between adjacent cells.
Biological function summary

These ephrins operate together with Eph receptors in a larger signaling complex. They influence cellular processes like boundary formation neural development and angiogenesis. Their interaction dictates the positioning and migration of cells during development. Ephrin B2 and Ephrin B3 specifically guide the formation of neural pathways and support vascularization while Ephrin B1 is important for craniofacial development. The dynamic interactions within these complexes highlight their regulatory role across various stages of organismal development.

Pathways

These ephrins and Eph receptors are integral to the Eph/ephrin signaling pathway and the MAPK signaling pathway. The Eph/ephrin pathway governs cellular repulsion and attraction mechanisms critical for tissue patterning. Ephrin B2 and Ephrin B3 through their interaction with EphB receptors influence these signaling cascades. In the MAPK pathway their activity affects cell proliferation and differentiation by interacting with proteins like RAS and RAF which contribute to signaling cascades that impact cell fate decisions.

These ephrins are associated with developmental defects and cancer. Mutations or dysregulation in Ephrin B1 can lead to craniofrontonasal syndrome characterized by abnormal facial and skeletal development. Ephrin B2 and Ephrin B3 have connections to tumor growth and metastasis often associated with aberrant angiogenesis. Interplay with proteins like VEGF in tumor environments highlights their role in promoting aberrant vascular networks contributing to cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development (PubMed : 7973638, PubMed : 8070404). Binding to Eph receptors residing on adjacent cells leads to contact-dependent bidirectional signaling into neighboring cells (PubMed : 7973638, PubMed : 8070404). Shows high affinity for the receptor tyrosine kinase EPHB1/ELK (PubMed : 7973638, PubMed : 8070404). Can also bind EPHB2 and EPHB3 (PubMed : 8070404). Binds to, and induces collapse of, commissural axons/growth cones in vitro (By similarity). May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
See full target information EFNB1 pY324

Additional targets

EFNB3 phospho Y318,EFNB2 phospho Y311
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 79:487 PubMed35984507

2022

WWOX inhibition by Zfra1-31 restores mitochondrial homeostasis and viability of neuronal cells exposed to high glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Carvalho,Sónia C Correia,Raquel Seiça,Paula I Moreira
View all publications
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Product promise

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