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AB119323

Anti-Ephrin B2 (phospho Y316) antibody

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(5 Publications)

Rabbit Polyclonal Ephrin B2 phospho Y316 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human EFNB2 pY316 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

EPLG5, HTKL, LERK5, EFNB2, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, HTK ligand, LERK-5, HTK-L

1 Images
Western blot - Anti-Ephrin B2 (phospho Y316) antibody (AB119323)
  • WB

Unknown

Western blot - Anti-Ephrin B2 (phospho Y316) antibody (AB119323)

All lanes:

Western blot - Anti-Ephrin B2 (phospho Y316) antibody (ab119323) at 1/500 dilution

Lane 1:

HT29 cell lysate

Lane 2:

HT29 cells, treated with UV and lysed

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human EFNB2 pY316 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P52799

Specificity

ab119323 recognizes endogenous Ephrin B2 only when phosphorylated at Tyrosine 316. May also react with Ephrin B1 and B3 as the immunogen sequence is also present in these proteins if phophorylated.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin B2 also called EFNB2 or ephrin-B2 is a transmembrane protein that plays important roles in cell signaling. The protein weighs approximately 37 kDa and it is expressed in various tissues like endothelial cells and neuronal tissues. Ephrin B2 binds to Eph receptors which are a part of the largest family of receptor tyrosine kinases. This interaction facilitates bidirectional signaling necessary for mediating cell communication.
Biological function summary

Ephrin-B2 serves as critical mediator for vascular development and nervous system maturation. It is an important player in angiogenesis where it functions through interaction with EphB receptors to regulate blood vessel formation and remodeling. Ephrin-B2 part of the larger ephrin family often acts in concert with other ephrins and Eph receptors to form signaling complexes that coordinate cell positioning and tissue architecture.

Pathways

The action of ephrin-B2 integrates into the Eph/ephrin signaling pathway and the VEGF signaling pathway. These pathways are important for cellular guidance and migration particularly in developing tissues. Ephrin B2's interaction with proteins like VEGF and its receptors emphasizes its role in angiogenic processes. Furthermore Ephrin-B2 interacts with other Eph receptor proteins to modulate cellular responses essential for proper vascular and neural functions.

Ephrin-B2's dysregulation associates with cancer and cardiovascular conditions. High expression of ephrin-B2 has been observed in tumor angiogenesis linking it to cancer progression through interactions with VEGF. Abnormal ephrin-B2 signaling may also relate to atherosclerosis involving disrupted endothelial cell function. These associations reveal ephrin-B2's potential as a therapeutic target for conditions involving aberrant vascular and cellular dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.. (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
See full target information EFNB2 pY316
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2921:155-179 PubMed40515989

2025

Application of Activity-Based Protein Profiling (ABPP) Strategy in the Identification of New Targets for Vascular Normalization and Binding Site Resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Yanchen Li

The Journal of surgical research 262:224-239 PubMed33039109

2020

Activation of EphrinB2 Signaling Promotes Adaptive Venous Remodeling in Murine Arteriovenous Fistulae.

Applications

Unspecified application

Species

Unspecified reactive species

Tun Wang,Jia Liu,Haiyang Liu,Shin-Rong Lee,Luis Gonzalez,Jolanta Gorecka,Chang Shu,Alan Dardik

International journal of molecular medicine 45:897-909 PubMed31985015

2020

Bidirectional ephrinB2‑EphB4 signaling regulates the osteogenic differentiation of canine periodontal ligament stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyue Zhu,Zongxiang Liu,Changyong Yuan,Yifan Lin,Yanqi Yang,Haiming Wang,Chengfei Zhang,Penglai Wang,Min Gu

Journal of cellular biochemistry 120:2876-2885 PubMed29236320

2017

Analgesic effects of microRNA-129-5p against bone cancer pain through the EphB1/EphrinB2 signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Nan Yu,Gui-Feng Liu,Long-Yun Li,Guo-Qing Zhao,Lin Liu,Xue-Feng Li

Nature communications 6:6625 PubMed25865237

2015

EphB4 forward signalling regulates lymphatic valve development.

Applications

WB

Species

Unspecified reactive species

Gu Zhang,John Brady,Wei-Ching Liang,Yan Wu,Mark Henkemeyer,Minhong Yan
View all publications
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Product promise

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