Skip to main content

Rabbit Polyclonal Ephrin B2 phospho Y316 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human EFNB2 phospho Y316 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Ephrin B2 (phospho Y316) antibody (AB119323), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human EFNB2 phospho Y316 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P52799

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Rat
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.

Alternative names

Recommended products

Rabbit Polyclonal Ephrin B2 phospho Y316 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human EFNB2 phospho Y316 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human EFNB2 phospho Y316 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P52799
Purification technique

Affinity purification Immunogen

Specificity

ab119323 recognizes endogenous Ephrin B2 only when phosphorylated at Tyrosine 316. May also react with Ephrin B1 and B3 as the immunogen sequence is also present in these proteins if phophorylated.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ephrin B2 also called EFNB2 or ephrin-B2 is a transmembrane protein that plays important roles in cell signaling. The protein weighs approximately 37 kDa and it is expressed in various tissues like endothelial cells and neuronal tissues. Ephrin B2 binds to Eph receptors which are a part of the largest family of receptor tyrosine kinases. This interaction facilitates bidirectional signaling necessary for mediating cell communication.

Biological function summary

Ephrin-B2 serves as critical mediator for vascular development and nervous system maturation. It is an important player in angiogenesis where it functions through interaction with EphB receptors to regulate blood vessel formation and remodeling. Ephrin-B2 part of the larger ephrin family often acts in concert with other ephrins and Eph receptors to form signaling complexes that coordinate cell positioning and tissue architecture.

Pathways

The action of ephrin-B2 integrates into the Eph/ephrin signaling pathway and the VEGF signaling pathway. These pathways are important for cellular guidance and migration particularly in developing tissues. Ephrin B2's interaction with proteins like VEGF and its receptors emphasizes its role in angiogenic processes. Furthermore Ephrin-B2 interacts with other Eph receptor proteins to modulate cellular responses essential for proper vascular and neural functions.

Associated diseases and disorders

Ephrin-B2's dysregulation associates with cancer and cardiovascular conditions. High expression of ephrin-B2 has been observed in tumor angiogenesis linking it to cancer progression through interactions with VEGF. Abnormal ephrin-B2 signaling may also relate to atherosclerosis involving disrupted endothelial cell function. These associations reveal ephrin-B2's potential as a therapeutic target for conditions involving aberrant vascular and cellular dynamics.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Ephrin B2 (phospho Y316) antibody (ab119323), expandable thumbnail

    Western blot - Anti-Ephrin B2 (phospho Y316) antibody (ab119323)

    All lanes: Western blot - Anti-Ephrin B2 (phospho Y316) antibody (ab119323) at 1/500 dilution

    Lane 1: HT29 cell lysate

    Lane 2: HT29 cells, treated with UV and lysed

    Predicted band size: 36 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com