AB53063

Anti-Ephrin B3 antibody

Name: Anti-Ephrin B3 antibody (ab53063) | Abcam
Brand: Abcam Limited
SKU: AB53063
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal Ephrin B3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EFNB3.

View Alternative Names

EPLG8, LERK8, EFNB3, Ephrin-B3, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8, LERK-8

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B3 antibody (AB53063)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B3 antibody (AB53063)

Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53063 at a 1 : 50 dilution.

Left image un-treated.

Right image treated with peptide.

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Western blot - Anti-Ephrin B3 antibody (AB53063)

All lanes:

Western blot - Anti-Ephrin B3 antibody (ab53063) at 1/500 dilution

Lane 1:

Extracts from SKOV3 cells

Lane 2:

Extracts from SKOV3 cells treated with peptide

Predicted band size: 35 kDa

Observed band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human EFNB3. The exact immunogen used to generate this antibody is proprietary information.

Q15768

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ephrin B3 also known as EFNB3 is a critical membrane-bound ligand for the Eph receptor tyrosine kinases. EFNB3 belongs to the ephrin family with a molecular mass of approximately 37 kDa. It predominantly expresses in the brain although researchers have detected it in other tissues as well. The protein plays a significant role in various cellular processes such as cell adhesion shape and migration.

Biological function summary

EFNB3 influences axon guidance and neural development. As part of a receptor-ligand complex it interacts with Eph receptors to regulate synaptic plasticity which is essential for learning and memory. This interaction also plays a role in mediating repulsive and attractive signals that guide neurons during developmental processes. EFNB3 exhibits distinct binding characteristics which determine specific cellular responses.

Pathways

EFNB3 influences both the ephrin signaling pathway and the nervous system development pathway. It engages with Eph receptors particularly EphB3 and EphA4 to trigger downstream signaling cascades that influence cellular structure and fate. The Eph-ephrin pathways are important in neurodevelopmental processes assisting in the formation of spatially regulated cell organization.

EFNB3 has connections to neurodegenerative diseases and cancer. Abnormal EFNB3 signaling can contribute to Alzheimer's Disease due to its role in neuronal connectivity and communication. The protein also associates with glioblastoma as it can influence tumor cell invasiveness and growth. Changes in ephrin signaling can alter normal cellular behavior which may progress to various pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).. (Microbial infection) Acts as a receptor for nipah virus and hendra virus.

See full target information EFNB3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 12: PubMed39767802

2025

Promoter Polymorphism (Allelic Variation) Affects Tacrolimus Treatment Efficacy by Modulating E2F6 Binding Affinity.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Zheng,Shengying Qin,Mingkang Zhong,Qinxia Xu,Cong Huai,Xiaoyan Qiu

Cancers 14: PubMed35565468

2022

Ephrin B Activate Src Family Kinases in Fibroblasts Inducing Stromal Remodeling in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mamatha Kakarla,Sathyavathi ChallaSivaKanaka,Mary F Dufficy,Victoria Gil,Yana Filipovich,Renee Vickman,Susan E Crawford,Simon W Hayward,Omar E Franco

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ephrin B3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ephrin B3 antibody (AB53063)

Western blot - Anti-Ephrin B3 antibody (AB53063)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise