AB9286

Anti-Epithelial Keratin Type I antibody [AE1]

Name: Anti-Epithelial Keratin Type I antibody [AE1] (ab9286) | Abcam
Brand: Abcam Limited
SKU: AB9286
Price: 510 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(14 Publications)

Mouse Monoclonal Epithelial Keratin Type I antibody. Suitable for IHC-P, IHC-Fr, WB and reacts with Mouse, Rat, Rabbit, Chicken, Cow, Monkey, Turtle samples. Cited in 14 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human KRT1.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AE1

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Mouse, Rat, Rabbit, Chicken, Cow, Monkey, Turtle

Applications

IHC-Fr, WB, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human KRT1.

P04264

Specificity

This antibody recognises most of the acidic (Type

keratins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turtle": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epithelial Keratin Type I also known as cytokeratin or CK is a family of cytoskeletal proteins found in the epithelial cells. These proteins are usually around 40-67 kDa in mass. Epithelial Keratin Type I is essential for structural integrity and mechanical strength of epithelial cells. It mainly expresses in epithelial tissues including skin hair and nails and serves a critical role in forming the intermediate filament network.

Biological function summary

These keratins provide stability to cell architecture and protect cells from mechanical and non-mechanical stresses. Epithelial Keratin Type I proteins form heterodimers by pairing with Type II keratins creating filaments that are part of a larger complex. These structures are vital for maintaining the correct function and shape of cells in tissues and they also play a role in cell signaling processes.

Pathways

Epithelial Keratin Type I participates in key pathways like the cytoskeletal signaling and epidermal growth factor (EGF) signaling. These pathways involve interactions with proteins such as keratin 8 and keratin 18. These keratins facilitate communication within cells and help mediate responses to external signals therefore influencing cell proliferation differentiation and apoptosis.

Mutations or dysfunctions in Epithelial Keratin Type I have links to skin diseases such as epidermolysis bullosa simplex and psoriasis. These conditions can result from altered interactions with other keratins such as keratin 5 and keratin 14 disrupting the normal function and structure of epithelial tissues. Such disorders highlight the importance of Epithelial Keratin Type I in maintaining healthy skin and its barrier functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Epithelial Keratin Type I

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 19:20220819 PubMed38585640

2024

Apigenin 7-glucoside impedes hypoxia-induced malignant phenotypes of cervical cancer cells in a p16-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Xiaoli Man,Qing Zhang,Xiaowu Wang,Yongli Yang

Science signaling 11: PubMed30301786

2018

The protein kinase p38α destabilizes p63 to limit epidermal stem cell frequency and tumorigenic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Kyung Choo,Stefan Kraft,Caterina Missero,Jin Mo Park

The Journal of pathology 245:114-125 PubMed29468680

2018

Proinflammatory role of blister fluid-derived exosomes in bullous pemphigoid.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Fang,Shuai Shao,Man Jiang,Erle Dang,Shengxian Shen,Jieyu Zhang,Pei Qiao,Caixia Li,Gang Wang

Scientific reports 4:5831 PubMed25060222

2014

TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoran Yin,Ting Zhang,Liangjun Qiao,Jia Du,Shuang Li,Hengguang Zhao,Fangfang Wang,Qiaorong Huang,Wentong Meng,Hongyan Zhu,Hong Bu,Hui Li,Hong Xu,Xianming Mo

The Journal of biological chemistry 288:23788-97 PubMed23836897

2013

Tuning of protein kinase circuitry by p38α is vital for epithelial tissue homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Celia Caballero-Franco,Min-Kyung Choo,Yasuyo Sano,Patcharee Ritprajak,Hiroaki Sakurai,Kinya Otsu,Atsushi Mizoguchi,Jin Mo Park

American journal of clinical pathology 84:697-704 PubMed2416215

1985

Monoclonal anti-keratin (AE1) reactivity in routinely processed tissue from 166 human neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

D V Spagnolo,S A Michie,G S Crabtree,R A Warnke,R V Rouse

Annals of the New York Academy of Sciences 455:307-29 PubMed2417518

1985

Monoclonal antibody studies of mammalian epithelial keratins: a review.

Applications

Unspecified application

Species

Unspecified reactive species

T T Sun,S C Tseng,A J Huang,D Cooper,A Schermer,M H Lynch,R Weiss,R Eichner

The American journal of pathology 117:53-63 PubMed6207735

1984

Immunohistochemical study of nasopharyngeal carcinoma using monoclonal keratin antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

S R Shi,M L Goodman,A K Bhan,B Z Pilch,L B Chen,T T Sun

Cancer 54:843-8 PubMed6204739

1984

Antikeratin antibodies in tumor diagnosis. Distinction between seminoma and embryonal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

H Battifora,K Sheibani,R R Tubbs,M I Kopinski,T T Sun

The Journal of cell biology 98:1388-96 PubMed6201491

1984

Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression.

Applications

Unspecified application

Species

Unspecified reactive species

R Eichner,P Bonitz,T T Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Epithelial Keratin Type I antibody [AE1]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Product promise