AB214192

Anti-epithelial Sodium Channel alpha antibody

Name: Anti-epithelial Sodium Channel alpha antibody (ab214192) | Abcam
Brand: Abcam Limited
SKU: AB214192

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(4 Publications)

Rabbit Polyclonal epithelial Sodium Channel alpha antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SCNN1A aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

SCNN1, SCNN1A, Epithelial sodium channel subunit alpha, Alpha-ENaC, ENaC subunit alpha, ENaCA, Epithelial Na(+) channel subunit alpha, Alpha-NaCH, Amiloride-sensitive sodium channel subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Sodium channel epithelial 1 subunit alpha

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

Synthetic Peptide within Human SCNN1A aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P37088

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The epithelial sodium channel alpha also known as the alpha subunit of the epithelial sodium channel (ENaC) is an important component of the ENaC channels. This protein forms part of the larger ENaC complex and plays an important role in sodium ion transport across epithelial cells. Typically the ENaC channel has a molecular mass of approximately 70 to 110 kDa. The channel is expressed in epithelial tissues such as the kidney lung and colon where it acts to regulate sodium excretion and reabsorption.

Biological function summary

The epithelial sodium channel alpha facilitates sodium ion entry into cells which impacts fluid balance blood pressure and extracellular fluid volume. The alpha subunit functions as part of a heterotrimeric complex consisting of alpha beta and gamma subunits forming the complete active channel. The coordinated action of these subunits ensures regulated ion transport which is essential for maintaining homeostasis in epithelial tissues.

Pathways

ENaC channels participate in the renin-angiotensin-aldosterone system and are involved in sodium reabsorption processes. This channel works closely with proteins like aldosterone to modulate its activity in response to changes in body fluid status. ENaC's ability to regulate sodium flux links it to key physiological functions in fluid and electrolyte balance pathways.

Alterations in ENaC function are linked to conditions such as Liddle's syndrome and cystic fibrosis. Liddle's syndrome involves mutations that lead to excessive sodium reabsorption resulting in hypertension. Furthermore ENaC function is disrupted in cystic fibrosis due to altered protein interactions contributing to imbalanced mucus composition. Proteins like cystic fibrosis transmembrane conductance regulator (CFTR) interact with ENaC in cystic fibrosis illustrating their interconnected role in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis (PubMed : 30251954, PubMed : 32729833, PubMed : 8023962, PubMed : 8278374, PubMed : 9792722). ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically (PubMed : 24124190, PubMed : 28710092, PubMed : 8278374). It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys (PubMed : 12107247). Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance (PubMed : 24124190, PubMed : 28710092).. Isoform 4. Not functional.

See full target information SCNN1A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 48:2218-2233 PubMed40490489

2025

Dark-phase melatonin administration does not reduce blood pressure but induces changes in parameters related to the control of the cardiovascular system in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Mauer Sutovska,Lubos Molcan,Peter Stefanik,Michal Zeman

Annals of translational medicine 11:4 PubMed36760249

2023

Involvement of miR-495 overexpression in the pathogenesis of bronchopulmonary dysplasia in preterm infants via the targeting of pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Fan Sun,Li Ma,Jian-Hui Li,Yuan Yang,Xiao-Hui Gong,Cheng Cai

Bioengineered 12:9390-9400 PubMed34823420

2021

Knocking down Sterol regulatory element binding protein 2 (SREBF2) inhibits the Serine Protease 8 (PRSS8) /sodium channel epithelial 1alpha subunit (SCNN1A) axis to reduce the cell proliferation, migration and epithelial-mesenchymal transformation of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Cai,Yumei Zhang,Xing Peng

Oncotarget 11:3590-3600 PubMed33062195

2020

NRXN1 as a novel potential target of antibody-drug conjugates for small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Takuma Yotsumoto,Keita Maemura,Kousuke Watanabe,Yosuke Amano,Yoko Matsumoto,Koichi Zokumasu,Takahiro Ando,Masanori Kawakami,Hidenori Kage,Jun Nakajima,Yutaka Yatomi,Takahide Nagase,Daiya Takai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-epithelial Sodium Channel alpha antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise