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AB322260

Anti-epithelial Sodium Channel alpha antibody [EPR28997-46]

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Rabbit Recombinant Monoclonal epithelial Sodium Channel alpha antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

SCNN1, SCNN1A, Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Alpha-ENaC, ENaCA

4 Images
Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)
  • WB

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (ab322260) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 73 kDa,75 kDa,36 kDa

true

Exposure time: 103s

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)
  • WB

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : MCF7(PMID : 18221536), HEK-293

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (ab322260) at 1/1000 dilution

Lane 1:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole lysate at 30 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole lysate at 30 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 103s

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)
  • WB

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35510563).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1 : 92 seconds, lane2 : 180 seconds

All lanes:

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (ab322260) at 1/1000 dilution

Lane 1:

22Rv1 (human prostate carcinoma epithelial cell) whole lysate at 30 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 73 kDa,75 kDa,100 kDa,150 kDa,36 kDa

false

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)
  • WB

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (AB322260)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human SCNN1D.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (ab322260) at 1/1000 dilution

Lane 1:

His-tagged human SCNN1A fragment

Lane 2:

His-tagged human SCNN1D fragment

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,45 kDa

false

Exposure time: 8s

  • Carrier free

    Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28997-46

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The epithelial sodium channel alpha also known as the alpha subunit of the epithelial sodium channel (ENaC) is an important component of the ENaC channels. This protein forms part of the larger ENaC complex and plays an important role in sodium ion transport across epithelial cells. Typically the ENaC channel has a molecular mass of approximately 70 to 110 kDa. The channel is expressed in epithelial tissues such as the kidney lung and colon where it acts to regulate sodium excretion and reabsorption.
Biological function summary

The epithelial sodium channel alpha facilitates sodium ion entry into cells which impacts fluid balance blood pressure and extracellular fluid volume. The alpha subunit functions as part of a heterotrimeric complex consisting of alpha beta and gamma subunits forming the complete active channel. The coordinated action of these subunits ensures regulated ion transport which is essential for maintaining homeostasis in epithelial tissues.

Pathways

ENaC channels participate in the renin-angiotensin-aldosterone system and are involved in sodium reabsorption processes. This channel works closely with proteins like aldosterone to modulate its activity in response to changes in body fluid status. ENaC's ability to regulate sodium flux links it to key physiological functions in fluid and electrolyte balance pathways.

Alterations in ENaC function are linked to conditions such as Liddle's syndrome and cystic fibrosis. Liddle's syndrome involves mutations that lead to excessive sodium reabsorption resulting in hypertension. Furthermore ENaC function is disrupted in cystic fibrosis due to altered protein interactions contributing to imbalanced mucus composition. Proteins like cystic fibrosis transmembrane conductance regulator (CFTR) interact with ENaC in cystic fibrosis illustrating their interconnected role in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
See full target information SCNN1A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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