AB324794

Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal epithelial Sodium Channel alpha antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

SCNN1, SCNN1A, Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Alpha-ENaC, ENaCA

4 Images

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

This data was developed using ab322260, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver

This blot was developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] (<a href='/en-us/products/primary-antibodies/epithelial-sodium-channel-alpha-antibody-epr28997-46-ab322260'>ab322260</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 73 kDa,75 kDa,36 kDa

true

Exposure time: 103s

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

This data was developed using ab322260, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : MCF7(PMID : 18221536) HEK-293

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Lane 1:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole lysate at 30 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell) whole lysate at 30 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 103s

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

This data was developed using ab322260, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35510563).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1 : 92 seconds lane2 : 180 seconds

All lanes:

Lane 1:

22Rv1 (human prostate carcinoma epithelial cell) whole lysate at 30 µg

Lane 2:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 73 kDa,75 kDa,100 kDa,150 kDa,36 kDa

false

Supplier Data

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

This data was developed using ab322260, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human SCNN1D.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Lane 1:

His-tagged human SCNN1A fragment

Lane 2:

His-tagged human SCNN1D fragment

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 23 kDa,45 kDa

false

Exposure time: 8s

Unconjugated

Anti-epithelial Sodium Channel alpha antibody [EPR28997-46]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28997-46

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab324794 is the carrier-free version of ab322260.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.

See full target information SCNN1A

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

Western blot - Anti-epithelial Sodium Channel alpha antibody [EPR28997-46] - BSA and Azide free (AB324794)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Product promise