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AB154530

Anti-EPLIN antibody

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(2 Publications)

Rabbit Polyclonal EPLIN antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIMA1 aa 450-750.

View Alternative Names

EPLIN, SREBP3, PP624, LIMA1, LIM domain and actin-binding protein 1, Epithelial protein lost in neoplasm

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-EPLIN antibody (AB154530)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPLIN antibody (AB154530)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling EPLIN with ab154530 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Western blot - Anti-EPLIN antibody (AB154530)
  • WB

Unknown

Western blot - Anti-EPLIN antibody (AB154530)

7.5% SDS PAGE

All lanes:

Western blot - Anti-EPLIN antibody (ab154530) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LIMA1 aa 450-750. The exact immunogen used to generate this antibody is proprietary information.

Q9UHB6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPLIN also known as Epithelial Protein Lost In Neoplasm is an actin-associated protein with significant roles in cell structure and function. It has a molecular mass of approximately 100 kDa. EPLIN exists in two isoforms: EPLIN-α and EPLIN-β with different expression patterns and functions. Various tissues express EPLIN with a high concentration seen in epithelial cells. EPLIN associates closely with the cytoskeletal framework stabilizing actin filaments and contributing to cellular architecture and motility.
Biological function summary

EPLIN plays a critical role in the regulation of cell dynamics. It operates as a structural component by binding to filamentous actin enhancing its stability and promoting bundled formations. It also suppresses actin filament depolymerization supporting cell adhesion and movement. As part of actin-associated complexes EPLIN interacts with proteins involved in cell signaling pathways such as RhoA influencing actin-binding properties and cellular responses. Its presence in these complexes implies a broader role in maintaining cellular integrity and mechanical support.

Pathways

Several elements illustrate how EPLIN integrates into signaling cascades. The actin cytoskeleton remodeling pathway prominently features EPLIN. Its interaction with RhoA and other GTPase family members controls actin filament dynamics influencing changes in cell shape and migration. The Wnt signaling pathway also involves EPLIN where it modulates actin cytoskeleton stability affecting cellular proliferation and differentiation. These pathways illustrate how EPLIN coordinates with proteins such as RhoA to facilitate critical cellular functions.

EPLIN exhibits associations with conditions such as cancer and fibrosis. In cancer reduced EPLIN expression correlates with increased cell migration and invasion contributing to tumor progression and metastasis. This relationship positions EPLIN as a potential biomarker for cancerous transformations. In fibrosis aberrant EPLIN expression affects cellular contractility and extracellular matrix composition implicating it in disease pathology. Connections with cancer-related proteins like E-cadherin and β-catenin further emphasize EPLIN's role in disease mechanisms influencing epithelial-mesenchymal transitions and cellular adhesion dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (PubMed : 12566430). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (By similarity).
See full target information LIMA1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinical Medicine Insights. Oncology 16:11795549221109493 PubMed35837368

2022

Overexpression of LIMA1 Indicates Poor Prognosis and Promotes Epithelial-Mesenchymal Transition in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ma,Yiqun Liao,Ziwen Gao,Wenyan Zhu,Jianbing Liu,Wandong She

Oncology reports 40:3040-3048 PubMed30226549

2018

Circadian clock gene Per2 downregulation in non‑small cell lung cancer is associated with tumour progression and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Run Xiang,Yue Cui,Yanping Wang,Tianpeng Xie,Xiaojun Yang,Zhu Wang,Juan Li,Qiang Li
View all publications
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