AB273070

Anti-EPO antibody

Name: Anti-EPO antibody (ab273070) | Abcam
Brand: Abcam Limited
SKU: AB273070

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(2 Publications )

Rabbit Polyclonal EPO antibody. Suitable for WB, IHC-P, ELISA and reacts with Recombinant full length protein - Human, Recombinant full length protein - Mouse, Rat, Recombinant full length protein samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPO.

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (AB273070)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (AB273070)

Paraffin-embedded rat brain tissue stained for EPO using ab273070 at 1/400 in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (AB273070)

Paraffin-embedded rat liver tissue stained for EPO using ab273070 at 1/400 in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

ELISA

Supplier Data

ELISA - Anti-EPO antibody (AB273070)

An ELISA plate is coated with 50 µL of EPO recombinant protein at concentration ranged from 0.125 µg/mL to 4 µg/mL. The coated protein is detected with EPO antibody at 1 µg/mL.

Supplier Data

Western blot - Anti-EPO antibody (AB273070)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-EPO antibody (ab273070) at 1/20000 dilution

All lanes:

Recombinant mouse EPO protein at 100 ng

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

IHC-P, ELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human EPO.

P01588

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant full length protein": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/1000 - 1/10000", "ELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant full length protein - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erythropoietin often called EPO or erythropoietin protein is a glycoprotein hormone with a mass of approximately 30.4 kDa. It plays a mechanical role in the production of red blood cells (erythropoiesis) by acting as a signaling molecule that binds the erythropoietin receptor on erythroid progenitor cells in the bone marrow. Erythropoietin is mainly expressed in the kidneys with some expression in the liver. It controls the proliferation and differentiation of precursor cells into mature red blood cells which are responsible for oxygen transport.

Biological function summary

Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.

Pathways

Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.

Erythropoietin is important in the treatment of anemia especially anemia associated with chronic kidney disease where natural EPO production decreases. Abnormal levels of erythropoietin are associated with conditions such as polycythemia vera a disorder characterized by excessive red blood cell production. EPO levels can be affected by production defects in the kidneys linking it to proteins like the von Hippel-Lindau tumor suppressor which regulates erythropoietin transcription under low oxygen conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass (PubMed : 28283061). Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3 (PubMed : 9774108).

See full target information EPO

Additional targets

EPO

Alternative Names

Erythropoietin, EPO, EP, EPO alpha, Epoetin, Erythropoetin, Erythropoietin precursor, MVCD2

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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Aging 15:12264-12274 PubMed37934569

2023

Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Tang,Xiuqin Li,Nan Yin,Ming Zhao,Qingchuan Hu,Peiyuan Lv

Respiratory research 22:52 PubMed33579290

2021

Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Fu,Chunyan Yang,Bing Chen,Kexing Zeng,Siyuan Chen,Yi Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EPO antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (AB273070)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody (AB273070)

ELISA - Anti-EPO antibody (AB273070)

Western blot - Anti-EPO antibody (AB273070)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Alternative Names

Publications (2)

Product promise