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AB308197

Anti-EPO antibody [HL1794]

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Rabbit Recombinant Monoclonal EPO antibody. Carrier free. Suitable for sELISA, WB, I-ELISA, IHC-P, Biological Activity and reacts with Transfected cell line - Human, Human, Cat, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Pig EPO.

View Alternative Names

Erythropoietin, EPO

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Biological Activity - Anti-EPO antibody [HL1794] (AB308197)
  • Biological Activity

Supplier Data

Biological Activity - Anti-EPO antibody [HL1794] (AB308197)

The cell proliferation of the TF-1 human erythroleukemic cell line induced by Human Erythropoietin/EPO and neutralization by ab308197. Proliferation elicited by Recombinant Human Erythropoietin/EPO (0.5 ng/mL) is neutralized by increasing concentrations of ab308197.

Indirect ELISA - Anti-EPO antibody [HL1794] (AB308197)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-EPO antibody [HL1794] (AB308197)

Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (ab308197) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) at 1/10000 dilution was used to detect the bound primary antibody.

Indirect ELISA - Anti-EPO antibody [HL1794] (AB308197)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-EPO antibody [HL1794] (AB308197)

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (ab308197) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) at 1/10000 dilution was used to detect the bound primary antibody.

Sandwich ELISA - Anti-EPO antibody [HL1794] (AB308197)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-EPO antibody [HL1794] (AB308197)

Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

Capture : ab308197 (5 µg/mL)

Detection : HRP-conjugated EPO antibody [HL1793] (1 µg/mL)

Sandwich ELISA - Anti-EPO antibody [HL1794] (AB308197)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-EPO antibody [HL1794] (AB308197)

Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

Capture : ab308197 (5 µg/mL)

Detection : HRP-conjugated EPO antibody [HL1793] (1 µg/mL)

Western blot - Anti-EPO antibody [HL1794] (AB308197)
  • WB

Supplier Data

Western blot - Anti-EPO antibody [HL1794] (AB308197)

HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (ab308197) diluted at 1/2000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-EPO antibody [HL1794] (ab308197) at 1/2000 dilution

Lane 1:

HepG2 cell lysate at 30 µg

Lane 2:

HepG2 lysate on conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21.3 kDa

false

Western blot - Anti-EPO antibody [HL1794] (AB308197)
  • WB

Supplier Data

Western blot - Anti-EPO antibody [HL1794] (AB308197)

Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (ab308197) diluted at 1/50000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-EPO antibody [HL1794] (ab308197) at 1/50000 dilution

Lane 1:

Escherichia coli expressed recombinant human EPO protein at 10 ng

Lane 2:

Escherichia coli expressed recombinant human EPO protein at 50 ng

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21.3 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody [HL1794] (AB308197)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody [HL1794] (AB308197)

Immunohistochemical analysis of paraffin-embedded cat kidney tissue labelling EPO with ab308197 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-EPO antibody [HL1794] (AB308197)
  • WB

Supplier Data

Western blot - Anti-EPO antibody [HL1794] (AB308197)

Separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-EPO antibody [HL1794] (ab308197) at 1/20000 dilution

All lanes:

Recombinant human EPO protein (CHO cells expressed) at 20 ng

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21.3 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1794

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Cat

Applications

Biological Activity, sELISA, WB, I-ELISA, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Pig EPO. The exact immunogen used to generate this antibody is proprietary information.

P49157

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "BiologicalActivity" : {"fullname" : "Biological Activity", "shortname":"Biological Activity"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/50000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "BiologicalActivity-species-checked": "testedAndGuaranteed", "BiologicalActivity-species-dilution-info": "", "BiologicalActivity-species-notes": "<p></p>" }, "Cat": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "BiologicalActivity-species-checked": "guaranteed", "BiologicalActivity-species-dilution-info": "", "BiologicalActivity-species-notes": "" }, "Recombinant full length protein - Human": { "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "BiologicalActivity-species-checked": "notRecommended", "BiologicalActivity-species-dilution-info": "", "BiologicalActivity-species-notes": "" }, "Transfected cell line - Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "5 µg/mL", "sELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/50000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "BiologicalActivity-species-checked": "notRecommended", "BiologicalActivity-species-dilution-info": "", "BiologicalActivity-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erythropoietin often called EPO or erythropoietin protein is a glycoprotein hormone with a mass of approximately 30.4 kDa. It plays a mechanical role in the production of red blood cells (erythropoiesis) by acting as a signaling molecule that binds the erythropoietin receptor on erythroid progenitor cells in the bone marrow. Erythropoietin is mainly expressed in the kidneys with some expression in the liver. It controls the proliferation and differentiation of precursor cells into mature red blood cells which are responsible for oxygen transport.
Biological function summary

Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.

Pathways

Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.

Erythropoietin is important in the treatment of anemia especially anemia associated with chronic kidney disease where natural EPO production decreases. Abnormal levels of erythropoietin are associated with conditions such as polycythemia vera a disorder characterized by excessive red blood cell production. EPO levels can be affected by production defects in the kidneys linking it to proteins like the von Hippel-Lindau tumor suppressor which regulates erythropoietin transcription under low oxygen conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
See full target information EPO
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Product promise

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For full details, please see our Terms & Conditions

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