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Rabbit Monoclonal EPO antibody. Carrier free. Suitable for sELISA, WB, I-ELISA, IHC-P, Biological Activity and reacts with Transfected cell line - Human, Human, Cat, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Pig EPO.

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Images

Biological Activity - Anti-EPO antibody [HL1794] (AB308197), expandable thumbnail
  • Sandwich ELISA - Anti-EPO antibody [HL1794] (AB308197), expandable thumbnail
  • Sandwich ELISA - Anti-EPO antibody [HL1794] (AB308197), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody [HL1794] (AB308197), expandable thumbnail
  • Western blot - Anti-EPO antibody [HL1794] (AB308197), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Pig EPO. The exact immunogen used to generate this antibody is proprietary information. Database link P49157

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISAWBI-ELISAIHC-PBiological Activity
Human
Predicted
Tested
Expected
Expected
Tested
Cat
Predicted
Expected
Expected
Tested
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Transfected cell line - Human
Expected
Tested
Not recommended
Not recommended
Not recommended

Expected
Expected

Species
Transfected cell line - Human
Dilution info
5 µg/mL
Notes

-

Predicted
Predicted

Species
Human, Cat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/2000 - 1/50000
Notes

-

Species
Human
Dilution info
1/2000 - 1/50000
Notes

-

Expected
Expected

Species
Cat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1 µg/mL
Notes

-

Expected
Expected

Species
Human, Cat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Cat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Cat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Target data

Function

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Alternative names

Erythropoietin, EPO

Recommended products

Rabbit Monoclonal EPO antibody. Carrier free. Suitable for sELISA, WB, I-ELISA, IHC-P, Biological Activity and reacts with Transfected cell line - Human, Human, Cat, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Pig EPO.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Pig EPO. The exact immunogen used to generate this antibody is proprietary information. Database link P49157
Carrier free
Yes
Clone number
HL1794
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Erythropoietin often called EPO or erythropoietin protein is a glycoprotein hormone with a mass of approximately 30.4 kDa. It plays a mechanical role in the production of red blood cells (erythropoiesis) by acting as a signaling molecule that binds the erythropoietin receptor on erythroid progenitor cells in the bone marrow. Erythropoietin is mainly expressed in the kidneys with some expression in the liver. It controls the proliferation and differentiation of precursor cells into mature red blood cells which are responsible for oxygen transport.

Biological function summary

Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.

Pathways

Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.

Associated diseases and disorders

Erythropoietin is important in the treatment of anemia especially anemia associated with chronic kidney disease where natural EPO production decreases. Abnormal levels of erythropoietin are associated with conditions such as polycythemia vera a disorder characterized by excessive red blood cell production. EPO levels can be affected by production defects in the kidneys linking it to proteins like the von Hippel-Lindau tumor suppressor which regulates erythropoietin transcription under low oxygen conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Biological Activity - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Biological Activity - Anti-EPO antibody [HL1794] (ab308197)

    The cell proliferation of the TF-1 human erythroleukemic cell line induced by Human Erythropoietin/EPO and neutralization by ab308197. Proliferation elicited by Recombinant Human Erythropoietin/EPO (0.5 ng/mL) is neutralized by increasing concentrations of ab308197.

  • Sandwich ELISA - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Sandwich ELISA - Anti-EPO antibody [HL1794] (ab308197)

    Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

    Capture: ab308197 (5 ?g/mL)

    Detection: HRP-conjugated EPO antibody [HL1793] (1 ?g/mL)

  • Sandwich ELISA - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Sandwich ELISA - Anti-EPO antibody [HL1794] (ab308197)

    Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below.

    Capture: ab308197 (5 ?g/mL)

    Detection: HRP-conjugated EPO antibody [HL1793] (1 ?g/mL)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO antibody [HL1794] (ab308197)

    Immunohistochemical analysis of paraffin-embedded cat kidney tissue labelling EPO with ab308197 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Western blot - Anti-EPO antibody [HL1794] (ab308197)

    HepG2 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (ab308197) diluted at 1/2000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-EPO antibody [HL1794] (ab308197) at 1/2000 dilution

    Lane 1: HepG2 cell lysate at 30 µg

    Lane 2: HepG2 lysate on conditioned medium at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 21.3 kDa

  • Western blot - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Western blot - Anti-EPO antibody [HL1794] (ab308197)

    Separated by 12% SDS-PAGE.

    All lanes: Western blot - Anti-EPO antibody [HL1794] (ab308197) at 1/20000 dilution

    All lanes: Recombinant human EPO protein (CHO cells expressed) at 20 ng

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 21.3 kDa

  • Indirect ELISA - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Indirect ELISA - Anti-EPO antibody [HL1794] (ab308197)

    Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (ab308197) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) at 1/10000 dilution was used to detect the bound primary antibody.

  • Western blot - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Western blot - Anti-EPO antibody [HL1794] (ab308197)

    Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (ab308197) diluted at 1/50000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

    All lanes: Western blot - Anti-EPO antibody [HL1794] (ab308197) at 1/50000 dilution

    Lane 1: Escherichia coli expressed recombinant human EPO protein at 10 ng

    Lane 2: Escherichia coli expressed recombinant human EPO protein at 50 ng

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 21.3 kDa

  • Indirect ELISA - Anti-EPO antibody [HL1794] (ab308197), expandable thumbnail

    Indirect ELISA - Anti-EPO antibody [HL1794] (ab308197)

    Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (ab308197) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) at 1/10000 dilution was used to detect the bound primary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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