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AB180791

Anti-EPO-R antibody [VP2E8]

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(1 Review)

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(1 Publication)

Mouse Monoclonal EPO-R antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Erythropoietin receptor, EPO-R, EPOR

2 Images
Flow Cytometry - Anti-EPO-R antibody [VP2E8] (AB180791)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-EPO-R antibody [VP2E8] (AB180791)

Flow cytometric staining of BOSC23 cells transiently transfected with an expression vector encoding either EPO-R (red curve) or an irrelevant protein black curve (control transfectant) labeling EPO-R with ab180791 at 1/200 dilution. A PE conjugated secondary antibody was used. A positive signal was obtained only with EPO-R transfected cells.

Capillary Gel Electrophoresis - Anti-EPO-R antibody [VP2E8] (AB180791)
  • CGE

Supplier Data

Capillary Gel Electrophoresis - Anti-EPO-R antibody [VP2E8] (AB180791)

Capillary Gel Electrophoresis (CGE) analysis of purified ab180791 monoclonal antibody. Lane 1 : molecular weight marker, Lane 2 : 2 μg of purified ab180791. Internal control bands (240 kDa / 7 kDa / 4,5 kDa).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

VP2E8

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab180791 was purified by protein G affinity chromatography from cell culture supernatants and verified by CGE
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Erythropoietin receptor (EPO-R) also known as EPOR plays an important role in erythropoiesis by mediating the effects of erythropoietin (EPO). EPO-R is a member of the cytokine receptor family with a mass of approximately 55 kDa. This receptor can primarily be found on the surface of erythroid progenitor cells in the bone marrow. Its presence is important for the final differentiation of these cells into mature erythrocytes.
Biological function summary

EPO-R functions by dimerizing upon EPO binding subsequently activating intracellular signaling pathways. It is a component of a larger signaling complex that recruits and activates various molecules initiating a cascade of events critical for cell survival and proliferation. This receptor signaling prevents apoptosis in erythroid progenitor cells promoting their growth and differentiation into red blood cells.

Pathways

EPO-R's activity falls within the JAK-STAT and PI3K-AKT signaling pathways. These pathways are pivotal in regulating hematopoiesis and cell survival. JAK2 acts as a direct partner to EPO-R and transduces the signal downstream while molecules like STAT5 get phosphorylated and mediate transcription of target genes. The PI3K-AKT pathway also contributes to the anti-apoptotic effect essential for erythrocyte development.

Altered EPO-R signaling associates with anemia of chronic disease and polycythemia vera. Anemic conditions often show reduced sensitivity or expression of EPO-R while mutations leading to EPO-R hypersensitivity link to polycythemia vera. Dysregulation of related proteins such as JAK2 also contributes to these conditions indicating the interconnected roles of these proteins in blood-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for erythropoietin, which mediates erythropoietin-induced erythroblast proliferation and differentiation (PubMed : 10388848, PubMed : 2163695, PubMed : 2163696, PubMed : 8662939, PubMed : 9774108). Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade (By similarity). In some cell types, can also activate STAT1 and STAT3 (PubMed : 11756159). May also activate the LYN tyrosine kinase (By similarity).. Isoform EPOR-T. Acts as a dominant-negative receptor of EPOR-mediated signaling.
See full target information EPOR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15259 PubMed40307488

2025

Development of a radiomic model to predict CEACAM1 expression and prognosis in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxue Zhang,Liping Han,Fangfang Nie,Huimin Zhang,Liming Li,Ruopeng Liang
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