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AB96695

Anti-Epoxide hydrolase antibody

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(4 Publications)

Rabbit Polyclonal Epoxide hydrolase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EPHX1 aa 1-200.

View Alternative Names

EPHX, EPOX, EPHX1, Epoxide hydrolase 1, Epoxide hydratase, Microsomal epoxide hydrolase, mEH

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (AB96695)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (AB96695)

ab96695, at a 1/500 dilution, staining Epoxide hydrolase in paraffin embedded SG xenograft by Immunohistochemical analysis.

Western blot - Anti-Epoxide hydrolase antibody (AB96695)
  • WB

Supplier Data

Western blot - Anti-Epoxide hydrolase antibody (AB96695)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Epoxide hydrolase antibody (ab96695) at 1/1000 dilution

Lane 1:

A431 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Lane 3:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EPHX1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P07099

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Alternative Products

Primary Antibodies

AB96774

Anti-Epoxide hydrolase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (AB96774)

  • 3
  • 1
Primary Antibodies

AB110307

Anti-Epoxide hydrolase antibody [15B2AD9]

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-Epoxide hydrolase antibody [15B2AD9] (AB110307)

  • 0
  • 0

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Secondary Antibodies

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Primary Antibodies

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Assay Kits

AB102530

Glutathione Peroxidase Assay Kit (Colorimetric)

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Primary Antibodies

AB47003

Anti-Cardiac Troponin I antibody

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Western blot - Anti-Cardiac Troponin I antibody (AB47003)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epoxide hydrolase also known as EH or EPHX is an enzyme that catalyzes the conversion of epoxides to dihydrodiols. This reaction is important for detoxifying harmful compounds making epoxide hydrolase an important player in maintaining cellular health. The enzyme typically has a molecular mass around 53 kDa. Epoxide hydrolase is expressed in various tissues with significant activity observed in the liver where it contributes to the metabolism of xenobiotics. Alternate forms such as epoxide hydrolase 2 also exist each with specific functions and expression patterns.
Biological function summary

The enzyme aids in the metabolic processing of xenobiotic and endogenous epoxides reducing their potential toxicity. Epoxide hydrolase operates by hydrolyzing epoxide substrates which can be harmful if accumulated. The enzyme is not part of a known permanent complex but interacts transiently with various substrates. Its biological role extends to maintaining the equilibrium between pro-inflammatory and anti-inflammatory mediators due to its influence on lipid epoxide metabolism.

Pathways

Epoxide hydrolase plays a role in the arachidonic acid metabolic pathway where it converts epoxide intermediates into more polar diols. This action regulates the balance of eicosanoids lipid signaling molecules vital for inflammation and immune responses. The enzyme also functions alongside other detoxification enzymes such as cytochrome P450s to facilitate the biotransformation of a wide array of compounds including pharmaceutical agents.

Epoxide hydrolase is linked to several conditions. Polymorphisms in the gene encoding epoxide hydrolase have associations with susceptibility to various cancers due to altered detoxification capacities. Abnormalities in enzyme activity are also associated with cardiovascular diseases as epoxide hydrolase regulates lipid mediators that modulate blood pressure and vascular tone. In these contexts the enzyme’s regulatory role intersects with proteins involved in inflammation and vascular biology like cytochrome P450s and vascular endothelial growth factor (VEGF) influencing disease progression and treatment outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water (By similarity). Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids (PubMed : 22798687). Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol (PubMed : 24958911). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).
See full target information EPHX1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Biology of reproduction 105:278-289 PubMed33855340

2021

Obesity alters the ovarian proteomic response to zearalenone exposure†.

Applications

Unspecified application

Species

Unspecified reactive species

M Estefanía González-Alvarez,Bailey C McGuire,Aileen F Keating

The Journal of cell biology 218:3697-3713 PubMed31604801

2019

Insulin activates intracellular transport of lipid droplets to release triglycerides from the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Mukesh Kumar,Srikant Ojha,Priyanka Rai,Alaumy Joshi,Siddhesh S Kamat,Roop Mallik

Molecular carcinogenesis 58:808-819 PubMed30644597

2019

Emerging role of EPHX1 in chemoresistance of acute myeloid leukemia by regurlating drug-metabolizing enzymes and apoptotic signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Cheng,Chongmei Huang,Gusheng Tang,Huiying Qiu,Lei Gao,Weiping Zhang,Jianmin Wang,Jianmin Yang,Li Chen

The Journal of clinical endocrinology and metaboli 96:2851-60 PubMed21715529

2011

Maternal smoking and fetal sex significantly affect metabolic enzyme expression in the human fetal liver.

Applications

WB

Species

Human

Peter J O'Shaughnessy,Ana Monteiro,Siladitya Bhattacharya,Paul A Fowler
View all publications
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Product promise

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