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AB96774

Anti-Epoxide hydrolase antibody

3

(1 Review)

|

(2 Publications)

Rabbit Polyclonal Epoxide hydrolase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EPHX1 aa 50-300.

View Alternative Names

EPHX, EPOX, EPHX1, Epoxide hydrolase 1, Epoxide hydratase, Microsomal epoxide hydrolase, mEH

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (AB96774)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (AB96774)

Immunohistochemical analysis of paraffin-embedded SG xenograft, using ab96774 at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Epoxide hydrolase antibody (AB96774)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Epoxide hydrolase antibody (AB96774)

Immunofluorescence analysis of methanol fixed HeLa, using ab96774 at 1/200 dilution (top image) and costained with Hoescht 33342 (bottom image).

Western blot - Anti-Epoxide hydrolase antibody (AB96774)
  • WB

Unknown

Western blot - Anti-Epoxide hydrolase antibody (AB96774)

Gel conc 10% (SDS PAGE)

All lanes:

Western blot - Anti-Epoxide hydrolase antibody (ab96774) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EPHX1 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

P07099

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Alternative Products

Primary Antibodies

AB96695

Anti-Epoxide hydrolase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (AB96695)

  • 0
  • 0
Primary Antibodies

AB110307

Anti-Epoxide hydrolase antibody [15B2AD9]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody [15B2AD9] (AB110307)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB28146

Anti-Cytochrome P450 2E1 antibody

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

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Assay Kits

AB102530

Glutathione Peroxidase Assay Kit (Colorimetric)

Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530)

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Primary Antibodies

AB47003

Anti-Cardiac Troponin I antibody

BOND RX™ Validated

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epoxide hydrolase also known as EH or EPHX is an enzyme that catalyzes the conversion of epoxides to dihydrodiols. This reaction is important for detoxifying harmful compounds making epoxide hydrolase an important player in maintaining cellular health. The enzyme typically has a molecular mass around 53 kDa. Epoxide hydrolase is expressed in various tissues with significant activity observed in the liver where it contributes to the metabolism of xenobiotics. Alternate forms such as epoxide hydrolase 2 also exist each with specific functions and expression patterns.
Biological function summary

The enzyme aids in the metabolic processing of xenobiotic and endogenous epoxides reducing their potential toxicity. Epoxide hydrolase operates by hydrolyzing epoxide substrates which can be harmful if accumulated. The enzyme is not part of a known permanent complex but interacts transiently with various substrates. Its biological role extends to maintaining the equilibrium between pro-inflammatory and anti-inflammatory mediators due to its influence on lipid epoxide metabolism.

Pathways

Epoxide hydrolase plays a role in the arachidonic acid metabolic pathway where it converts epoxide intermediates into more polar diols. This action regulates the balance of eicosanoids lipid signaling molecules vital for inflammation and immune responses. The enzyme also functions alongside other detoxification enzymes such as cytochrome P450s to facilitate the biotransformation of a wide array of compounds including pharmaceutical agents.

Epoxide hydrolase is linked to several conditions. Polymorphisms in the gene encoding epoxide hydrolase have associations with susceptibility to various cancers due to altered detoxification capacities. Abnormalities in enzyme activity are also associated with cardiovascular diseases as epoxide hydrolase regulates lipid mediators that modulate blood pressure and vascular tone. In these contexts the enzyme’s regulatory role intersects with proteins involved in inflammation and vascular biology like cytochrome P450s and vascular endothelial growth factor (VEGF) influencing disease progression and treatment outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water (By similarity). Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids (PubMed : 22798687). Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol (PubMed : 24958911). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity).
See full target information EPHX1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of food biochemistry 46:e14500 PubMed36515171

2022

Laminarin ameliorates alcohol-induced liver damage and its molecular mechanism in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Guo,Lingfeng Zhu,Yaping Zhou,Shuai Han,Yunyun Cao,Zuomin Hu,Yi Luo,Liyuan Bao,Xiuxiu Wu,Dandan Qin,Qinlu Lin,Feijun Luo

Evidence-based complementary and alternative medic 2016:3696232 PubMed27293457

2016

Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A.

Applications

WB

Species

Unspecified reactive species

Carmen Ortiz,Luisa Morales,Miguel Sastre,William E Haskins,Jaime Matta
View all publications
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Product promise

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