Rabbit Polyclonal EPPIN antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPPIN.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.91% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human EPPIN. Database link O95925
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility.
Alternative names
SPINLW1, WAP7, WFDC7, EPPIN, Eppin, Cancer/testis antigen 71, Epididymal protease inhibitor, Protease inhibitor WAP7, Serine protease inhibitor-like with Kunitz and WAP domains 1, WAP four-disulfide core domain protein 7, CT71
Recommended products
Rabbit Polyclonal EPPIN antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EPPIN.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.91% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human EPPIN. Database link O95925
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EPPIN short for Epididymal protease inhibitor is a protein that plays an important structural and mechanical role in sperm functionality. With a molecular mass of approximately 19 kDa EPPIN is mainly expressed in the male reproductive system particularly in the epididymis and on the surface of spermatozoa. This protein is recognized by several names including SPINLW1 which stands for Sperm protein with a Kunitz-type inhibitor domain. EPPIN interacts with the surface of sperm to regulate sperm motility and maturation.
- Biological function summary
EPPIN influences sperm function by acting as part of a multi-protein complex essential for sperm motility and stability. It is known to bind with Semenogelin (SEMG) a major protein in semen impacting its hydrolytic and inhibitory action. Through this interaction EPPIN contributes to the liquefaction of semen and the regulation of sperm forward motility. It modulates calcium signaling in spermatozoa which is critical for the regulation of sperm activity post-ejaculation.
- Pathways
EPPIN has been implicated in pathways associated with reproductive processes. It plays a role in the regulation of the calcium signaling pathway which involves other proteins like CatSper which are essential for sperm capacitation and hyperactivation. EPPIN through its interaction with SEMG indirectly influences the proteolytic pathways that govern seminal fluid conversion from gel to liquid state necessary for the sperm to become fully motile.
- Associated diseases and disorders
EPPIN has connections with male infertility and potential as a target for male contraception. Its interaction with SEMG has been studied in relation to infertility cases where sperm motility is compromised. Furthermore altered levels or functions of EPPIN could link to abnormal semen viscosity contributing to difficulties in natural conception. Researchers are exploring EPPIN as a target for non-hormonal male contraceptives leveraging its pivotal role in sperm functionality without affecting hormone levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPIN antibody (ab185699)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling EPPIN with ab185699 at 1/200. Magnification: 400x.
Immunocytochemistry/ Immunofluorescence - Anti-EPPIN antibody (ab185699)
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells using ab185699. Blue DAPI for nuclear staining.
Western blot - Anti-EPPIN antibody (ab185699)
All lanes: Western blot - Anti-EPPIN antibody (ab185699) at 1/500 dilution
Lane 1: THP1 cell lysate
Lane 2: Mouse liver lysate
Lane 3: Mouse testis lysate
Predicted band size: 15 kDa
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