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AB247172

Anti-EPPK1 antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal EPPK1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EPPK1.

View Alternative Names

EPIPL, EPPK1, Epiplakin, 450 kDa epidermal antigen

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)

Formalin-fixed, paraffin-embedded human urinary bladder tissue stained for EPPK1 with ab247172 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for EPPK1 with ab247172 at a 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-EPPK1 antibody (AB247172)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EPPK1 antibody (AB247172)

PFA fixed, Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling EPPK1 using ab247172 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)

Formalin-fixed, paraffin-embedded human skin tissue stained for EPPK1 with ab247172 at a 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPK1 antibody (AB247172)

Formalin-fixed, paraffin-embedded human liver tissue shows absence of immunoreactivity for EPPK1 when incubated with ab247172 at a 1/1000 dilution (negative control).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EPPK1. The exact immunogen used to generate this antibody is proprietary information.

P58107

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EPPK1 also known as epiplakin is a large protein with a molecular mass of approximately 725 kDa. It is part of the plakin family and functions as a cytolinker protein. EPPK1 is fundamentally present in epithelial tissues and its expression is noted in various organs including the skin stomach and esophagus. This protein aids in the stabilization of cytoskeletal structures by crosslinking intermediate filaments within the cells.
Biological function summary

EPPK1 contributes to the structural integrity of epithelial cells by promoting filamentous network organization. It acts as a component in complexes that assemble the cytoskeleton's intermediate filament network significantly impacting cellular resilience and shape. EPPK1's interactions are important for maintaining cellular architecture and play an important role in cell adhesion processes.

Pathways

EPPK1 plays a significant role in both cytoskeletal organization and the cellular response to stress. It is part of pathways that involve keratin proteins which are necessary for maintaining epithelial scaffold resilience. Its interaction with proteins like desmoplakin further integrates EPPK1 into desmosome complex pathways that are essential for cohesion among epithelial cells.

Mutations or altered expression of EPPK1 have been linked to skin disorders such as epidermolysis bullosa and other keratin-associated conditions. This protein's anomalies might influence the desmosome machinery with possible interactions affecting other proteins like plakoglobin which is also associated with cellular adhesion integrity. Understanding EPPK1’s involvement in these conditions could pave the way for better therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoskeletal linker protein that connects to intermediate filaments and controls their reorganization in response to stress (PubMed : 15671067, PubMed : 23398049, PubMed : 27206504). In response to mechanical stress like wound healing, is associated with the machinery for cellular motility by slowing down keratinocyte migration and proliferation and accelerating keratin bundling in proliferating keratinocytes thus contributing to tissue architecture (PubMed : 23398049, PubMed : 27206504). However in wound healing in corneal epithelium also positively regulates cell differentiation and proliferation and negatively regulates migration thereby controlling corneal epithelium morphogenesis and integrity. In response to cellular stress, plays a role in keratin filament reorganization, probably by protecting keratin filaments against disruption. During liver and pancreas injuries, plays a protective role by chaperoning disease-induced intermediate filament reorganization (By similarity).
See full target information EPPK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed36231039

2022

A Ca-Mediated Switch of Epiplakin from a Diffuse to Keratin-Bound State Affects Keratin Dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Ratajczyk,Corinne Drexler,Reinhard Windoffer,Rudolf E Leube,Peter Fuchs
View all publications
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